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All Journal UNEJ e-Proceeding Nusantara Bioscience
Hailu Weldekiros Hailu
Department of Biology, College of Science, Bahir Dar University, Bahir Dar, Amhara, Ethiopia
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Review: Phylogenetic Similarity based on amino acid sequence and Molecular characterization of 3-Phytase from Klebsiella pneumonia ASR1 Sajidan .; Hailu Weldekiros Hailu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Phytase is an enzyme that catalyzes the release of phosphomonester group in phytate, thereby producing lower forms of myo-inositol phosphates and inorganic phosphate.  Phytase has an important role in animal nutrition which improves the bioavailability of nutrients.  Bacteria are one of the potential sources of phytase. For this reason, more screening of phytase producing bacteria strains from environment is needed. The other sources of phytase are fungi, plants, and some animal tissues. The PhyK protein is a phytase enzyme from Klebsiella pneumonia ASR1 and expressed in Escherichia coli, which was sequenced and characterized as a 42 kDa [1], and Crystallized [2]. The objectives are to perform phylogenetic similarity and molecular evolutionary analysis of phytase and to determine active site protein motif of phytase from different sources. The sequence from PhyK was analyzed with Basic Local Alignment Search Tools (BLAST) at http://www.ncbi.nlm.nih.gov  and ClustalW Multiple Sequence analysis program (EMBL-EBI) at http://www.ebi.ac.uk/Tools/msa/clustalw2/ to get similarity of amino acid sequence and phylogenetic trees for protein.  The result shows that PhyK protein from Klebsiella pneumonia ASR1 is 91% similar with PhyK from Raoultella terrigena (emb|AJ575300.1|), 94% similar with histidine acid phosphatase family protein from Klebsiella pneumoniae 342, and 69% similar with glucose-1-phosphatase precursor Agp from Pantoea ananatis AJ13355 (BAK10365.1).The deduced amino acid sequence of the phyK gene from Klebsiella pneumonia ASR1, although containing the functional residues of histidine acid phosphatases, displayed only 31% overall homology with appA from Escherichia coli O104:H4 str. C227-11 (EGT68141.1), 30% similar with glucose-1-phosphatase from Pantoea agglomerans (gb| ABD85282.1|) and 26% to phytase from Aspergillus fumigatus (AAU93517.1). This suggests that PhyK represents a novel subfamily histidine acid phytate-degrading enzyme and is clearly distinct from other previously characterized members of this family. Keywords: Bioinformatics, BLAST, 3-Phytase, PhyK protein, Phylogenetic Similarity
Transplantation of carica (Vasconcellea pubescens) at various altitudes of Mount Lawu, Central Java with treatment of shade and different types of fertilizers ALFATIKA PERMATASARI; SUGIYARTO SUGIYARTO; MARSUSI MARSUSI; HAILU WELDEKIROS HAILU
Nusantara Bioscience Vol. 7 No. 1 (2015)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n070102

Abstract

Abstract: Permatasari A, Sugiyarto, Marsusi, Hailu WH. 2015. Transplantation of carica (Vasconcellea pubescens) at various altitudes of Mount Lawu, Central Java with treatment of shade and different types of fertilizers. Nusantara Bioscience 7: 6-14. Ex-situ conservation efforts to increase crop production can be done by means of transplantation. The aim of this study was to assess the effect of altitude, shade, and type of fertilizer and their interactions to the performance of carica (Vasconcellea pubescens) vegetative plants transplanted to Mount Lawu, Central Java, Indonesia. The research was conducted in divided plot design at three altitudes (± 1400, 1600 and 1800 m asl.) with shade treatment as main plots and fertilizer (manure, Zwavelzuur ammonium/ZA fertilizer, combination of manure with Ammonium fertilizer, and control) as subplots. Parameters of vegetative variability were observed. The results of the performance of carica vegetative plant were analyzed using ANOVA followed by Duncan's test at 5% standard test. It was observed that an increase in altitude caused slower growth of carica vegetative parts, but better vegetative growth performance in the presence of shade. For the fertilizers, the best growth parameters were obtained in the treatment of manure with ZA fertilizer. Interaction between altitude, shade, and type of fertilizer significantly influenced the performance of carica vegetative parameters. The best growth parameters are at an altitude of 1400 m above sea level with shade treatment and manure combined with ZA fertilizer.
Co-Authors ALFATIKA PERMATASARI MARSUSI MARSUSI Sajidan SUGIYARTO SUGIYARTO