Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Jurnal Health and Sport YARSI Medical Journal
Asaad Maidin
Unknown Affiliation
Health Professions Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
KloningOpen Reading Frame (orf) ESAT-6 (Early Secretory Antigenic Target-6) Mycobacterium tuberculosis ke Escherichia coliBL21 (DE3) Agus, Rosana; Maidin, Asaad; Hatta, Mochammad; S.Retnoningrum, Debbie
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.365 KB) | DOI: 10.33476/jky.v16i3.245

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis is an infectious diseases leading to significant death toll in most partof the world. Vaccination with BCG, a TB vaccine, is still a common practice until now. In general, the people in Indonesia receive BCG vaccine during their early childhood, but the efficacy of the vaccine would not last long to adulthood, which allow themto get potential latent TB infection. This latent TB infection might be detected by tuberculin skin test (TST), however, the weakness is that false positive reactions are commonly found due to cross reaction between antibodies produced during BCG vaccination and purified protein derivative (PPD). Alternatively, its detection could be performed by identifying immunodominant antigen to M. tuberculosis. The Early Secretory AntigenicTarget-6 (ESAT-6) for antibody based serological detection with high sensitivity and specificity could also be applied. The purpose of this study was to clone the open reading frame (orf) of ESAT-6 from Mycobacterium tuberculosis into Escherichia coli BL21 (DE3). In this method, orf ESAT-6 was ligated to the expression vector pET-32b and transformed into E. coli BL21 (DE3). Characterization of clones was carried out by cutting the recombinant plasmid using restriction enzymes BamH1 and XhoI. The result showed that three colonies with recombinant plasmid pET-32b-ESAT-6 were obtained. Sequencing of the DNA insert was then performed using the universal T7 primer. Characterization of white colonies with restriction enzymes showed two bands i.e. 288 bp and 5900 bp for orf ESAT-6 and pET32b vector respectively. BLAST analysis of sequence showed 100% homology.
(PATTERN OF RAPD (Random Amplified Polymorphic DNA) Mycobacterium tuberculosis RESISTAN RIFAMPISIN (RIF) AND ISONIAZID (INH) IN MAKASSAR) ., Purwanta; Maidin, Asaad
Jurnal Health and Sport VOL 7, NO 1, 2013
Publisher : JURNAL HEALTH AND SPORT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.284 KB)

Abstract

Tujuan penelitian ini adalah untuk mempelajari pola RAPD M. tuberculosis (MTB) isolat klinik resisten isoniazid (INH) dan rifampisin (RIF) di Kota Makassar dengan metode Polymerase Chain Reaction Random Amplified Polymorphic DNA (PCR-RAPD). Penelitian dilakukan di laboratorium Bioteknologi Pusat Kegiatan Penelitian (PKP) Universitas Hasanuddin, dengan jumlah sampel masing-masing isolat klinik resisten rifampisin (RIF) 6 sampel, dan isolat klinik resisten isoniazid 7 sampel. Penelitian meliputi ekstraksi DNA dengan kit komersial Wizard, amplifikasi DNA dan elektroforesis. Primer yang digunakan 5 primer yaitu OPN-02 (5'ACCAAGGGGCA, 70% G+C), OPN-09 (5'TGCCGGCTTG 3',70% G+C), OPN-20 (5'GGTGCTCCGT 3', 70% G+C), BG-65 (5' CTCGAGCGGC 3',80% G+C), BG-66 (5'CGACGCTGCG 3'. 80% G+C). Diversitas genetik isolat dianalisa dengan metode Dendro UPGMA: A Dendogram Construction Utility dari Valive, S.G. (2009). Hasil penelitian menunjukkan bahwa 5 primer yang digunakan memberikan hasil amplifikasi yang jelas. Pita DNA yang berhasil diamplifikasi berukuran antara 200 sampai 1500 bp. Variasi genetik dilihat dari perbedaan jumlah fragmen, ukuran fragmen, jumlah pita polimorfik dan persentase (%) polimorfik. Persentase polimorfik tertinggi ditemukan pada kelompok isolat klinik MTB resisten Rif berkisar 80 - 100% atau rata-rata 93,76%, sedangkan pada kelompok isolat klinik MTB resisten INH berkisar 50 - 100% atau rata-rata 90%. Analisis dendogram dengan metode UPGMA antara isolat RIF dan INH memberikan nilai koefisien kekerabatan 0-40% atau terdapat keragaman populasi 60-100%. Tingginya polimorfisme disebabkan oleh mutasi pada gen pengkode resistensi obat anti TB.
Co-Authors Mochammad Hatta Purwanta . Rosana Agus S.Retnoningrum, Debbie