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All Journal TEKMAPRO Journal of Industrial Engineering and Management MEDISAINS Berkala Kedokteran Nusantara Medical Science Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma Majalah Kesehatan FKUB Health Notions As-syifaa Jurnal Farmasi IJEBD (International Journal Of Entrepreneurship And Business Development) Alami Journal (Alauddin Islamic Medical) Journal Al-sihah: The Public Health Science Journal YARSI Medical Journal Jurnal Biomedika Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Teknik Industri Terintegrasi (JUTIN) Indonesian Andrology and Biomedical Journal Journal of Health and Nutrition Research Proceeding Muhammadiyah International Public Health and Medicine Conference Journal of Health Sciences and Medical Development

Mochammad Hatta

Unknown Affiliation

Author-ID : 278632

Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

Published : 23 Documents Claim Missing Document

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Peningkatan Pelayanan Service Advisor dengan Menerapkan Metode Quality Function Deployment di Auto 2000 Hr. Muhammad Surabaya Hatta, Mochammad
JURNAL TEKMAPRO Vol 3, No 1 (2008): JURNAL TEKMAPRO
Publisher : JURNAL TEKMAPRO

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.815 KB)

Abstrak Â Industri yang bergerak dibidang jasa mengalami peningkatan, demikian juga dengan industri jasa bengkel. Kenaikan tersebut sangat berdampak pada persaingan bisnis antar badan usaha sejenis. Oleh karena itu bagi jasa bengkel Auto 2000 juga ingin tetap eksis dan berkembang, dan mengedepankan kualitas pelayanan, karena kualitas pelayanan sudah menjadi tolak ukur bagi setiap konsumen pengguna jasa tersebut. Dalam pengolahan data yang digunakan adalah Cluster Random Sampling, variabel yang digunakan dalam penelitian ini adalah fasilitas service, penerimaan service, pelaksanaan service serta penyerahan dan tindak lanjut service, dan masing-masing variabel terdiri atas beberapa sub variabel. Hasil penelitian diperoleh bahwa kecermatan petugas service dalam menanggapi keluhan pelanggan adalah kebutuhan yang paling dipentingkan oleh para pelanggan dengan nilai tertinggi 4,49. Kemudian perlu diketahui juga berapa kesenjangan/gap antara kepuasan dengan harapan pelanggan. Dari hasil analisa diperoleh bahwa kebutuhan pelanggan dalam kemudahan membuat perjanjian service menepati urutan pertama dengan nilai gap sebesar -0,20. Sedangkan untuk nilai gap kualitas pelayanan secara keseluruhan adalah sebesar â0,0553 yang artinya kualitas belum memenuhi harapan pelanggan. Kata Kunci : Keputusan, Pelayanan dan Kualitas

KloningOpen Reading Frame (orf) ESAT-6 (Early Secretory Antigenic Target-6) Mycobacterium tuberculosis ke Escherichia coliBL21 (DE3) Agus, Rosana; Maidin, Asaad; Hatta, Mochammad; S.Retnoningrum, Debbie
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Tuberculosis (TB) caused by Mycobacterium tuberculosis is an infectious diseases leading to significant death toll in most partof the world. Vaccination with BCG, a TB vaccine, is still a common practice until now. In general, the people in Indonesia receive BCG vaccine during their early childhood, but the efficacy of the vaccine would not last long to adulthood, which allow themto get potential latent TB infection. This latent TB infection might be detected by tuberculin skin test (TST), however, the weakness is that false positive reactions are commonly found due to cross reaction between antibodies produced during BCG vaccination and purified protein derivative (PPD). Alternatively, its detection could be performed by identifying immunodominant antigen to M. tuberculosis. The Early Secretory AntigenicTarget-6 (ESAT-6) for antibody based serological detection with high sensitivity and specificity could also be applied. The purpose of this study was to clone the open reading frame (orf) of ESAT-6 from Mycobacterium tuberculosis into Escherichia coli BL21 (DE3). In this method, orf ESAT-6 was ligated to the expression vector pET-32b and transformed into E. coli BL21 (DE3). Characterization of clones was carried out by cutting the recombinant plasmid using restriction enzymes BamH1 and XhoI. The result showed that three colonies with recombinant plasmid pET-32b-ESAT-6 were obtained. Sequencing of the DNA insert was then performed using the universal T7 primer. Characterization of white colonies with restriction enzymes showed two bands i.e. 288 bp and 5900 bp for orf ESAT-6 and pET32b vector respectively. BLAST analysis of sequence showed 100% homology.

Deteksi Mutasi Gen Gyrase A Porphyromonas Gingivalis Resisten terhadap Ciprofloxacin berdasarkan teknik Polymerase Chain Reaction E.Rieuwpassa, Irene; Hatta, Mochammad
Jurnal Kedokteran YARSI Vol 17, No 1 (2009): JANUARI - APRIL 2009
Publisher : Lembaga Penelitian Universitas YARSI

One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.

IDENTIFIKASI DNA LEPTOSPIRA SP PADA SAMPEL AIR DAN TANAH DI KOTA MAKASSAR Mursalim, Muhammad Fadhlullah; Hatta, Mochammad
Jurnal Ilmiah As-Syifaa Vol 10, No 1 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Leptospirosis is a zoonotic disease that can be found all over the world, caused by a pathogenic leptospira genus. This study aims to identify the presence of Leptospira bacterial DNA in water and soil at several locations in Makassar. This research was conducted in microbiology laboratory Faculty of Medicine Hasanuddin University. A total of 16 samples were taken consisting of water and soil samples taken from four locations in Makassar City namely eggplant market, slums, TPA (final landfill) tamangappa and slaughterhouse (RPH). Samples were then tested using the nested RT-PCR method . The results of this study showed that from 16 samples tested showed one sample with positive to the presence of Leptospira sp bacteria obtained from the soil found in slums located in makassar.

The Effectiveness of Spiritual Qur’anic Emotional Freedom Technique (SQEFT) Intervence Against the Change of Brief Psychiatric Rating Scale (BPRS) on Patient with Schizophrenia Lilin Rosyanti; Indriono Hadi; Jayalangkara Tanra; Asadul Islam; Mochammad Hatta; Veny Hadju; Faizal Idrus; Kusman Ibrahim
Health Notions Vol 2, No 8 (2018): August
Publisher : Humanistic Network for Science and Technology (HNST)

Dimension of spiritual nursing demands the role of nurse in order to give holistic treatment, combine physical, psychological, social, and spiritual dimension. The psychospiritual intervention: SQEFT is intervention of psychospiritual combination that combines between spiritual Qur’anic therapy and psychological therapy of EFT, as additional spiritual intervention for patient with schizophrenia by not reducing any medical treatment program that the patient does. The objective of this research, in order to examine the influence of SQEFT Intervence Against The Change of BPRS on Patient with Schizophrenia. There were 7 mental patients skizoprenia participate of this research, who were diagnosed schizophrenia with psychiatry of DSM-IV-TR by psychiatric doctor with preliminary BPRS value scale in less than 60. The result test of Bonferroni showed that three groups had significant difference. There was a significant difference between non SQFET and SQEFT1, SQEFT2 with pvalue of 0.000. This difference showed that BPRS value moved lower significantly before being given SQEFT intervention, after being given SQEFT in stage 1 and SQEFT in stage 2. BPRS value that was lower showed psychological condition, schizophrenia patient’s cognitive condition which were better. Keywords: Psychospiritual intervention, SQEFT, BPRS, Nursing holistic, Schizophrenia

Analisis Secara Bakteriologik, Imunoserologik dan Polymerase Chain Reaction terhadap Sampel Pasien Suspek Limfadenitis tuberkulosis Nur Afiah; Mansyur Arief; Mochammad Hatta
Nusantara Medical Science Journal Volume 5 No. 1 Januari - Juni 2020
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v5i1.8442

Latar Belakang: Penelitian ini bertujuan menganalisis kemampuan tes imunoserologi, bakteriologi dan sitologi untuk mendeteksi Mycobacterium tuberculosis berdasarkan tes Polymerase Chain Reaction (PCR) pada penderita suspek limfadenitis tuberkulosa. Penelitian dilakukan secara cross sectional pada 35 sampel di RS Dr. Wahidin Sudirohusodo, RSD Labuang Baji, Laboratorium Patologi Anatomi Swasta dan Laboratorium Biologi Molekuler Fakultas Kedokteran Unhas. Ditemukan hasil positif paling banyak berturut-turut pada tes sitologi (80%), tes ICT (68,5%), tes PCR (62,8%), tes apusan basil tahan asam (BTA) (31,5%). Sensitivitas dan spesifisitas tes sitologi (72,3% dan 7,69%), tes ICT (63,64% dan 23,08%), dan tes apusan BTA (22,73% dan 58,85%) terhadap PCR. Sedangkan sensitivitas dan spesifisitas kombinasi tes Mycotec TB dengan BTA (22,73% dan 84,62%), kombinasi tes ICT dengan sitologi (50% dan 30,77%) dan kombinasi tes BTA dengan sitologi (22,73% dan 61,54%). Tes sitologi mempunyai sensitivitas yang paling tinggi terhadap PCR lalu diikuti oleh tes ICT, tes apusan BTA, sedangkan spesifisitasnya paling tinggi pada tes apusan BTA, lalu tes ICT dan tes sitologi. Nilai sensitivitas kombinasi tes ICT dengan sitologi lebih tinggi daripada kombinasi tes ICT dengan BTA dan kombinasi tes BTA dengan tes sitologi BTA yang mempunyai nilai yang sama.

The Analysis of Vitamin D Receptor Protein on Salmonella typhi infection in acute recurrent cases in endemic area in Eastern Indonesia Fatmawati Annisa Syamsuddin; Mochammad Hatta; Firdaus Hamid; Rosdiana Natzir; Ahyar Ahmad; Burhanuddin Bahar
Biomedika Vol 14 No 2 (2021): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v14i2.1298

The host susceptibility mechanisms such as Vitamin D Receptor (VDR) is involved in the modulation of macrophage function and may possibly correlate with immunity disease including the severity of typhoid fever symptoms. The study aimed to assess the VDR Protein expression in the serums of recurrent acute typhoid fever (RATF) patients and compares it with typhoid fever (TF) patients, and healthy persons (HP). The study employed 30 RATF patients and 30 TF patients selected from primary health centres and hospitals in Eastern Indonesia as the endemic area. All the samples were obtained from several health centers in South Sulawesi, Southeast Sulawesi, Central Sulawesi, East Kalimantan and Papua and then collected in the sample bank Biology Laboratory of Molecular Immunology, Faculty of Medicine, Hasanuddin University. As a comparison, 30 samples of healthy persons were also selected from the Blood Transfusion Unit in Makassar, South Sulwesi Indonesia. The profile of VDR Protein was analyzed with Enzyme-linked Immunosorbent Assay (ELISA). VDR protein content data on RATF and TF were designed according to completely randomized design T test. Subsequently, it correlated to Pearson correlation to determine the interaction between Widal titre and VDR protein levels. A comparison between Widal titre and VDR Protein level was also made to identify the correlation. It was found that the mean of VDR protein expression of RATF was 13,44 ng/mL, the mean of VDR protein expression of TF was 24,88 ng/mL, and the mean of VDR protein expression of HP was 43,49 ng/mL. The correlation results between RATF-TF Widal titre and VDR protein level indicated a negative correlation with p-value of 0,004. There were significant differences in the VDR expression in the RATF, TF, and HP. RATF VDR expression lower than TF and HP and there was also a correlation between Widal titre with VDR Protein expression.

The Use of Multiplex PCR Reactions to Identification of Lactic Acid Bacteria of Dangke Nurwilda Kaswi; Mochammad Hatta; Rizalinda Sjahril
Berkala Kedokteran Vol 19, No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v19i1.15723

Abstract: Lactic acid bacteria can be isolated from various types of milk including dairy products. Dangke is an Indonesian traditional cheese made by Enrekang community, South Sulawesi. This study aims to identify the lactic acid bacteria by multiplex PCR method which are isolated from dangke obtained from Enrekang Regency. This type of research used descriptive research with laboratory observation methods was performed on 30 cups of dangke are obtained from the markets in 5 sub-districts producing dangke in Enrekang Regency: they are the sub-districts of Cendana, Enrekang, Anggeraja, Alla, and Baraka. The results showed that Lactobacillus acidophilus was the most identified (33.33%) compared to Lactobacillus plantarum (16.67%). Based on the results of the research that has been carried out, it can be concluded that the lactic acid bacteria have been identified in dangke samples obtained from several sub-districts producing dangke in Enrekang Regency are Lactobacillus acidophilus and Lactobacillus plantarum species. Thus, we suggest that this laboratory technique is used to confirm the various species of lactic acid bacteria so it can prove more rapid and exactly that dangke is the rich content of lactic acid bacteria as probiotic candidate.

Exploring the Streptococci Variants in Children’s Oral Cavity, Its Microbiome Diversity Juniati Binti Lukman; Zaraswati Dwyana; Mochammad Hatta; Cyril B. Romero
Journal of Health Sciences and Medical Development Vol. 2 No. 02 (2023): Journal of Health Sciences and Medical Development
Publisher : The Indonesian Institute of Science and Technology Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56741/hesmed.v2i02.264

This study aimed to identify the variants of the Streptococcus genus in the oral cavity of healthy individuals. Streptococcus is one of the normal flora, which is part of the lactic acid bacteria (LAB) in the oral cavity. LAB has the ability to produce antimicrobial agents, regulate the host's immune response, and inhibit the growth of pathogenic bacteria. The presence of LAB in the oral cavity is crucial in maintaining oral health and preventing periodontal diseases. This study used observational cross-sectional design with 40 healthy elementary school children as subjects. Swabs were taken from their oral cavities and sent to the laboratory for PCR and sequencing tests. The results revealed that isolates 3S, 4S, 9S, 10S, 14S, and 15S were identified as Streptococcus dysgalactiae, while isolates 1S, 8S, and 9S were identified as Streptococcus mitis. Additionally, isolates 2S, 5S, 7S, 11S, 12S, and 13S were identified as Streptococcus salivarius, isolate 6S was identified as Streptococcus agalactiae, and isolate 17S was identified as Streptococcus oralis. These findings provide valuable information on the identification of oral microbiota, particularly Streptococcus genus variants, which can potentially serve as an alternative therapy for periodontal diseases.

ISOLASI METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) DARI NARES ANTERIOR PASIEN PRE-OPERASI ORTOPEDI Ilhamjaya, Andi Meutiah; Sjahril, Rizalinda; Johan, Muhammad Phetrus; Rasita, Yoeke Dewi; Mochammad Hatta; Andi Alfian Zainuddin; Patellongi, Ilhamjaya; Arden, Ferdinand
Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Vol 5 No 3 (2023): December
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v5i3.140

Methicillin-Resistant Staphylococcus aureus (MRSA) dapat menyebabkan infeksi yang didapat di rumah sakit (HA-MRSA) atau yang didapat di masyarakat (CA-MRSA). Dengan dampak morbiditas dan mortalitas yang lebih tinggi. Dalam kondisi sumber daya yang terbatas, ketersediaan deteksi cepat masih belum memadai di beberapa fasilitas laboratorium di Indonesia. Seringkali, membutuhkan waktu lebih dari 48 hingga 72 jam untuk mengidentifikasi MRSA di laboratorium. Oleh karena itu, deteksi cepat menggunakan metode alternatif sangatdiperlukan untuk mempersingkat waktu yang diperlukan untuk mengidentifikasinya. Semakin cepat teridentifikasi saat skrining nares anterior pada pasien pre operasi ortopedi, maka semakin cepat pula tindakan pencegahan maupun pengendalian infeksi yang dapat dilakukan di Rumah Sakit. Penelitian ini merupakan penelitian observasional dengan desain cross sectional study. Pada penelitian ini dilakukan pengambilan sampel swab nares anterior pasien rencana bedah ortopedi di RSUP Dr. WahidinSudirohusodo dan RS Universitas Hasanuddin Makassar menggunakan medium transport Amies gel agar. Setelah itu dilakukan inokulasi langsung ke media CHROMOGENIC AGAR MRSA, hasil identifikasi dapat diketahui segera setelah 18-24 jam inkubasi pada media tersebut. Hasil penelitian menunjukkan dari 184 sampel yang diperoleh, proporsi temuan karier MRSA dari swab nares anterior pasien pre-operasi ortopedi di RSUP Wahidin Sudirohusodo Makassar adalah 66 pasien (38.4%), dan 8 pasien (4.6%) MSSA, sedangkan 98 pasien (57%)terdeteksi non aureus. Adapun karakteristik pasien yang mempunyai hubungan bermakna dengan temuan MRSA pada swab nares anterior pasien pre-operasi ortopedi yaitu karakteristik administrasi, riwayat rawat inap RS 3 bulan sebelumnya, dan riwayat pengunaan antibiotik 6 bulan terakhir (p value :0.001; 0.015; 0.002). Hasil penelitian disimpulkan bahwa pemanfaatan media CHROMOGENIC AGAR MRSA sebagai salah satu metodekultur untuk skrining MRSA pada nares anterior pasien pre-operasi ortopedi dapat menjadi pilihan yang efektifdan efisien dalam aplikasi klinis di lingkungan Rumah Sakit. Hal ini mengurangi penggunaan banyak bahan dilaboratorium, memudahkan dalam identifikasi langsung koloni oleh karena kekhasan koloni MRSA padamedia CHROMOGENIC AGAR, serta mempersingkat waktu yang diperlukan untuk mengidentifikasi MRSApada pasien, sehingga dapat memudahkan tindakan pencegahan dan pengendalian penyebaran MRSA dilingkungan Rumah Sakit, membantu mengurangi lama rawat inap pasien maupun mengurangi kemungkinanpenggunaan antibiotik jangka panjang pada pasien.

Co-Authors Ahmad Hadi Modi Ahyar Ahmad Andi Alfian Zainuddin Andi Rofian Sultan Andi Saifah Arden, Ferdinand Ardiputra, Muh Akbar Asaad Maidin Asadul Islam Baedah Madjid Bagus Wicaksono Bahagia, Dwi Bambang Sardi Burhanuddin Bahar Cyril B. Romero E.Rieuwpassa, Irene Elli Hidayati Ema Alasiry, Ema Fadhilah Faizal Idrus Farid, Monika Fitria Fatimah Fatmawati Annisa Syamsuddin Firdaus Hamid Guli, Musjaya Hamid, Firdaus HAMIDAH Haruna, Nadyah Herman, Sriyana Hursepuny, Valentine Ilhamjaya Patellongi Ilhamjaya Patellongi Ilhamjaya, Andi Meutiah Indriono Hadi Jayalangkara Tanra Johan, Muhammad Phetrus Juniati Binti Lukman Kaswi, Nurwilda Kusman Ibrahim Kusnawati, Ida Lestari, Esa Lilin Rosyanti Lisa Tenriesa Madjid, Baedah Mansyur Arief Muh. Nasrum Massi Muhammad Basir Mursalim, Muhammad Fadhlullah Murtiani, Farida Ni Made Adi Tarini Nur Afiah Nurmawati Nurmawati Prawiro, Sumarno Reto Rahma Rahma Rahmawati, Nurul Dina Ramadanil Pitopang Rasita, Yoeke Dewi Retno Sari Riza Linda Rizalinda Sjahril Rizalinda Sjahril rosamarlina, rosamarlina Rosana Agus Rosdiana Natzir S.Retnoningrum, Debbie Safri Safri Sonny T. Lisal Sultan, Andi Rofian Suryani As’ad Syahrul Rauf Tjahjani, Ida Kusnawati Veny Hadju Widiantari, Aninda Dinar Yoeke Dewi Rasita Zaraswati Dwyana

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