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UJI EFIKASI BEBERAPA ISOLAT BAKTERI ENTOMOPATOGEN TERHADAP KECOA (Orthoptera) Periplaneta americana (L.) DAN Blatella germanica (L.) DALAM SKALA LABORATORIUM Monaliza Sekar Rini; Rully Rahadian; mochamad Hadi; Deni Zulfiana
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Cockroaches are the insect vectors of disease that can cause adverse effects on human health. Control cockroaches excessive use of insecticides can lead to residues in the environment and resistance cockroach. Therefore it is necessary to control the use of alternatives such as by biological agents such as entomopathogenic bacteria. This research used two isolates of entomopathogenic bacteria isolated from Spodoptera litura were found dead, Bacillus thuringiensis IPBCC collection and sterile distilled water as a control. Tests conducted by the spray and bait method at a concentration of 108. The results showed that all isolates entomopathogenic bacteria used in this research can cause mortality of cockroaches. Morphological observation and Koch's postulates test showed that the mortality of cockroaches likely caused by bacterial isolates were granted. SP4 bacterial isolates using spray method provides the highest mortality was 26.67% of the P. americana and 80% against B. germanica. Isolates of B. thuringiensis using bait influence the highest mortality of 10% of the P. americana and 6.67% against B. germanica. LT-50 was the most effective method of treatment of bacterial isolates SP4 spray against B. germanica was 2 hours 30 minutes 46 seconds.Keywords: Biological control, entomopathogenic bacterial, efficacy test

KEANEKARAGAMAN DAN KELIMPAHAN JENIS BURUNG PADA TAMAN KOTA SEMARANG, JAWA TENGAH Bimo Ghifari; Mochamad Hadi; Udi Tarwotjo
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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The City Park is conceived as a space that contains the elements of nature and landscape which is caused by vegetation diversity, activity and artificial elements that are provided as a social and recreational facilities, as well as a breathing space in the city. The Semarang State Park is one of the green open area that are also used by birds. On the other hand the city park has a problem that threatens the existence of birds especially the uncontrolled development. The diversity of the species of birds can be used as indicators of environmental quality, because their life is influenced by the physical, chemical, and biological cchanges in the environment. The research objective was to determine the diversity and abundance of the bird species, determine the composition of the guild of bird species, and to determine the status of the scarcity of birds in the city garden of Semarang. The research was conducted in July-August 2016, where observation was carried out in six state parks in Semarang using the point count method. The analysis of the index used are the abundance of species, species diversity index, evenness index, similarity index, chi square test and hucthinson method.Where795 individuals of birds were observed, covering 27 species and 17 families. The diversity of bird species index categorized moderately were 2.19 to 2.33, except on Park Madukoro which had the lowest category. The birds were categorized evenly with the index 0.74-0.83, except in the Madukoro Wildlife where the categories were fairly even. The composition of the most abundant guild is a group of insectivorous which is 41%. There are 27 species of birds in all six State Parks in Semarang which however have the status of Least Concern by the IUCN. Based on Government Regulation No. 7 of 1999, there are four species of birds included into the category which are protected.Keywords: State Parks, bird, diversity, abundance, composition guild

STRUKTUR KOMUNITAS MIKROARTROPODA TANAH DI LAHAN PENAMBANGAN GALIAN C ROWOSARI, KECAMATAN TEMBALANG, SEMARANG Wiatri Larasati; Rully Rahadian; mochamad Hadi
Jurnal Akademika Biologi Vol. 5 No. 1 Januari 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Rowosari miningsite isthe ‘C’ type of excavation area in Semarang. Mining activities may affect environmental disturbance, including on soil fauna. This research aims to determine the community structure of soil microarthropods in Rowosari mining area. The research was conducted in August-September 2015. Soil samples were taken on diagonal plot of 5x5 m2 with a five-point sampling on two stations, namely Post Mining Area (PoMA) and Pre Mining Area (PeMA). The analysis used in the research are relative abundance index, Shannon-Weiner diversity index, evenness index, and Sorensen similarity index. The results shows that there are 360-660 individuals/m2 from 10 ordo and 24 taxa found in Rowosari Excavation Site. The highest relative abundance index is Carabidae (22,22) in PoMA station and Prostigmata (21,21) in PeMA station. The diversity both station belongs to medium category (2,43-2,45). The highest evenness index is 0,95. The similarity taxa of soil microarthropods in two stations are categorized as medium. Community structure of soil microathropods in Post Mining Area and Pre Mining Areawere no significant differences. Keywords: Community structure, Soil microarthropods, MiningexcavationC.

KEANEKERAGAMAN NGENGAT DI WANA WISATA GONOHARJO, LIMBANGAN, KENDAL, JAWA TENGAH Nanang Kamaludin; Mochamad Hadi; Rully Rahadian
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Moths is insect in Order Lepidoptera of Nocturnal habit. Moths help to polinate the flower were blossom in the night. The relationship of moths and plants can be used as bio-indicators of environmental changes in the Gonoharjo forest habitats. The objective of this study are to compare the abundance and diversity of members of moth (Lepidoptera) in various habitats and to determine the status of rare and endemic species that are found in Wana Wisata Gonoharjo. This study used light traps method for moth in four different habitats, i.e., secondary forests, riparian area and forests of pine forest and coffee plantation. The research was conducted over two months during October-November 2012. The analysis used were relative abundance, diversity index, Huctheson test, evennes index and similarity index. Threety nine moth species from 10 families were found in 4 habitats of Wana Wisata Gonoharjo. The highest number of moth species found in secondary forest (30 species) and the lowest one found in the pine forests (6 species). Statistically, there were differences within the types of habitat diversity. Evennes indices in all habitats of Wana Wisata Gonoharjo were relatively high. In general, type of habitat affects species diversity of moths. Keyword : Diversity, moth and Gonoharjo forest.

Pengaruh Rendaman Eceng Gondok (Eichhornia crassipes [Mart.] Solms) Terhadap Daya Predasi Mesocyclop jakartensis Alekseev Dheanda Absarina; Rully Rahadian; mochamad Hadi
Jurnal Akademika Biologi Vol. 4 No. 1 Januari 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Aedes aegypti has been reported as vectors of diseases, including dengue fever (dengue hemorrhagic fever) and Chikungunya. Various efforts have been done to overcome A. aegypti either physically, chemically and biologically control. However, these efforts have not been successful in reducing the density of the vector. The objectives of this study was to compare predation of M. jakartensis on A. aegypti mosquito larvae in several type of water i.e., well water (control), hyacinth infusion and hay infusion. The research method used complete randomized factorial design. Its effect on predation capability test of M. jakartensis used bioassay method on 25 initial instar larvae and 5 individuals of M. jakartensis. Predation capabilities test was analyzed using one-way ANOVA test. Correlation of the chemical factors on capabilities of M. jakartensis predation on the treatments was analyzed statistically using the Spearman test. The results show that predation capabilities of M. jakartensis on A. aegypti larvae are as follows, control > Hyacinth (3: 7) > Hay (1: 9) > Hyacinth (1:9). The percentage of predation for each treatment are 93,3; 72,0; 66,7 and 64,0%, respectively. Statistically, predation capabilities of M. jakartensis on A. aegypti larvae in each treatmens is not significantly different (P > 0.05). Hyacinth infusion is unproven in improving the predation of M. jakartensis on A. aegypti larvae, but it does not inhibit capabilities of M. jakartensis predation. Keywords : Aedes aegypti, Eichhornia crassipes, Mesocyclop jakartensis

EKTOPARASIT (PROTOZOA DAN HELMINTHES) PADA LALAT DI PASAR JOHAR DAN PASAR PETERONGAN KOTA SEMARANG ECTOPARASIT (PROTOZOAN DAN HELMINTHES) ON THE FLIES AT JOHAR AND PETERONGAN MARKETS SEMARANG CITY Meilinda Hilda Ryani; Retno Hestiningsih; Mochamad Hadi
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 4 (2017): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Flies can play a role as mechanic vector in the transmission of disease. This is due to the behavior of flies that like dirty and moist places. Traditional markets have potential in the presence of flies because there are many food source for flies such as garbage, vegetable residue, and fresh or rotten meat. The purpose of this study was to determine the diversity of ectoparasites (protozoa and helminthes) on the fly at Johar and Peterongan Markets in Semarang. This was an explorative study which was conducted by survey method and laboratory examination with cross sectional approach. The population of the study were all flies contained in Johar and Peterongan. Sampling was done by accidental sampling at three spots (garbage dump, meat selling area and fish selling area). Flies caught in both markets were 338 heads consisting of 74% Chrysomya megacephala and 26% Musca domestica. The results showed that 6.25% of the flies caught in the Johar market positively carried the worm Trichuris sp. egg and hookworm egg, whereas 31,25% of flies in Peterongan market possitively carried the cyst of Blastocystis sp., Chilomestix sp., and Isospora sp. as well as the egg of worm Capillaria sp.

PENGARUH PENAMBAHAN CYROMAZINE PADA PAKAN TERHADAP PERTUMBUHAN POPULASI LARVA Musca domestica PADA KOTORAN UNGGAS Septi Wulandari; Retno Hestiningsih; Mochamad Hadi
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 1 (2017): JANUARI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Flies are vector of disease living near human settlements and farm. In poultry, cyromazine larvicides used to control the growth of the flies by mixing into poultry feed. This application method leaving residues risk in eggs and poultry meat. Therefore, the effectiveness of flies growth inhibition due cyromazine need to be investigated. The purpose of this study was to analyze the effect of adding cyromazine in feed on the population growth of Musca domestica larvae in poultry manure. This research is a quasi experimental with posttest group only. The population is all rearing result of the first generation Musca domestica larvae, with a sample of 720 larvae. Cyromazine dose used is 1%, Poultry manure used is chicken manure and quail.The analytical method used is the analysis Growth Index (GI), Relative Growth Index (RGI), Kruskal Wallis test, and Mann Whitney test. Results showed that the GI value on chicken manure, quail manure, husk, mixture of husk and concentrate is 0,48; 0,61; 0; 1 and value RGI 48%, 61%, 0%, 100%. Statistically, they are differences of mortality in chicken manure and quail manure (p value = 0.000 <0.05).The use cyromazine 1% in the feed is not effective to inhibit the growth of Musca domestica larvae.

PENGARUH VARIASI UMPAN AROMA TERHADAP JUMLAH LALAT YANG TERPERANGKAP DALAM PERANGKAP WARNA KUNING (Studi di Kandang Sapi Dusun Tegalsari Desa Sidomukti Kecamatan Bandungan Kabupaten Semarang) Dewi Mustikawati; Martini Martini; Mochamad Hadi
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 4 (2016): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Some species of the fly is a vector borne diseases that contribute to public health problems. A fly can carry 65 million micro-organisms, it is not surprising if many people sick because their food was contaminated by the flies. Therefore, it is necessary to study the methods of control are effective and safe for the environment by using variations of flavor baits (durian, jackfruit, mango, frambozen, and pineapple).Research was aimed to analyze the effect of baits flavour variation to the number of flies caught in a yellow trap. The design study is Pseudo Experiment with design Post Test Only Control Group Design. Research was conducted in a cowshed, Hamlet Tegalsari, Village Sidomukti, District Bandungan, Semarang for 2 days. The experiment was repeated 5 times with the same time of day.The results showed the number of flies caught in a trap without bait is 4, trap with pineapple bait is 13.8, trap with jackfruit bait is 14.6, trap with durian bait is 47.2, trap with mango and frambozen bait is 19.8. The species of flies was trapped is 603 tail Fannia spp and 2 tail Stomoxys calcitrans sp. Data was analyzed by Kruskal Wallis test at the significance level α = 0.05. Results of calculation by Man Whitney test is there are different variations of flavour baits to the number of flies trapped. The order of variation from the lowest to the highest are control, pineapple, jackfruit, mango, frambozen, and durian.

EFIKASI EKSTRAK DAUN ZODIA (Evodia suaveolens) PELARUT HEKSANA DALAM MEMATIKAN LARVA INSTAR III Aedes aegypti Didi Setiyadi; Martini Martini; Mochamad Hadi
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 8, No 2 (2020): EDITION JULY 2020
Publisher : Universitas Tribhuwana Tunggadewi

DHF is a disease caused by a virus which is transmitted through the bite of the Aedes aegypti mosquito and is one of the health problems in Indonesia. Efforts to decide the transmission of DHF by controlling the population of Aedes aegypti at the larval stage using larvaside temepos. Long-term use of temephos causes Aedes aegypti resistance and environmental damage. One of the plants that has the potential as botanical larvaside is the leaves of zodia (Evodia suaveolens). The purpose of this study was to prove the efficacy of zodia leaves of hexane solvent for killing A. aegypti larvae. This type of research is a pure experiment with the post test only control group design. The research sample is A. aegypti stage III susceptible strain. There are 7 groups consisting of 1 negative control (aquades), positive control (1 ppm temepos) and 5 treatment groups (zodia leaf extract). Data on larval mortality were counted 24 hours after treatment. The results of the one way ANOVA test showed that there were differences in mortality between test groups with a p value: 0.001 α (0.05). Zodia leaf extract probit test results showed LC50-24 hour and LC90-24 hour values of 0,443 ppm and 0,788 ppm. Zodia leaf extract with hexane solvent has efficacy as Aedes aegypti larvicide.

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