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Isolasi dan Identifikasi Bakteri Micrococcus luteus dan Staphylococcus epidermidis pada Ambing Sapi Aceh (Isolation and Identification Micrococcus luteus and Staphylococcus epidermidis Bacteria on the Udder of Aceh Cattle) Usma Aulia; Teuku Zahrial Helmi; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 2 (2022): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i2.8630

Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Micrococcus luteus dan Staphylococcus epidermidis pada ambing sapi aceh. Penelitian ini menggunakan metode Carter yang dianalisis secara deskriptif. Sampel yang digunakan adalah 10 ambing sapi aceh yang terdapat di UPT Hewan Coba Fakultas Kedokteran Hewan Universitas Syiah Kuala. Kode sampel dibuat berdasarkan nomor yang terdapat pada telinga sapi. Pengambilan sampel dilakukan dengan menggunakan swab steril, kemudian dikultur dalam media nutrient broth (NB) dan diinkubasi selama 24 jam pada suhu 37 °C. Selanjutnya dilakukan penanaman pada media selektif mannitol salt agar (MSA) dan media blood agar (BA) lalu diinkubasi kembali selama 24 jam dengan suhu 37 °C. Koloni bakteri yang tumbuh terpisah pada media MSA dan BA diamati morfologi koloni bakteri, pewarnaan Gram, uji katalase dan uji biokimia (manitol dan glukosa). Hasil penelitian menunjukkan bahwa dari 10 sampel swab ambing diidentifikasi 3 isolat Micrococcus luteus dan 7 isolat Staphylococcus epidermidis. Kesimpulan penelitian ini adalah bakteri Staphylococcus epidermidis lebih sering berada pada ambing sapi aceh dibandingkan dengan keberadaan Micrococcus luteus (The aims of this study was to isolate and identify Micrococcus luteus and Staphylococcus epidermidis bacteria on the udder of aceh cattle. This study used Carter method which analyzed descriptively. The samples used were 10 udder of aceh cattle at UPT Hewan Coba faculty of Veterinary of Syiah Kuala University. The samples code created based on the number tag on the cattle’s ear. The sample was taken from cattle’s udder by using sterile swab cultured in nutrient broth (NB) media and incubated for 24 hours at 37 °C. Furthermore, cultured in mannitol salt agar (MSA) media and blood agar (BA) media then re-incubated for 24 hours at 37 °C. Bacterial colonies that grew apart on MSA and BA media were observed the morphology of bacterial colonies, Gram stained, catalase test and biochemical test (mannitol and glucose). The result of this study indicated that from 10 samples, identified 3 samples are Micrococcus luteus and 7 samples are Staphylococcus epidermidis. The conclusions of this study was Staphylococcus epidermidis bacteria was more common on the udder of aceh cattle than Micrococcus luteus bacteria).

EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Usma Aulia; Sri Wahyuni; Gholib Gholib; Dasrul Dasrul; Mulyadi Adam; Erdiansyah Rahmi; Abdullah Hamzah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.

Laporan kasus: Infeksi toxocara canis pada anjing domestik (Canis familiaris) Usma Aulia; Anna Zukiaturrahmah; Juliadi Ramadhan; Ario Ridho Gelagar
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 11 No. 2 (2023): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v11i2.7903

Dogs are one of the human pets that are often affected by parasitic infections, both endoparasites (helminths and protozoa) and ectoparasites (fleas, ticks, fleas, and mites). Endoparasites that are often reported are helminth cases, namely Ancylostomiasis, Toxocariasis, Dipylidiasis, Trichuriasis and Nekatoriasis. Toxocara canis is one of the most widespread public health and economically important zoonotic parasitic infections humans share with dogs. Meanwhile, the ectoparasites, especially fleas, that are often encountered are Ctenocephalides canis. The method used to detect the presence of worms is native, centrifugation, and floating feces examination and Mc Master Chamber calculation to determine the severity of the worm infection. In this case, blood tests were also carried out. Flea examination is carried out by identifying ectoparasite wet preparations. The results of a floating stool examination revealed Toxocara canis worm eggs. The ectoparasite obtained after laboratory examination was the flea Ctenocephalides canis. The treatment used to treat helminth disease is by administering worm medicine, while for flea disease it can be done by anti-flea injection. Prevention can be done by administering worm medicine regularly and keeping the dog's cage and environment clean.

Laporan Kasus: Pyometra Pada Anjing Ras Labrador : Case Report: Pyometra in Labrador Dog Anna Zukiaturrahmah; Juliadi Ramadhan; Ario Ridho Gelagar; Usma Aulia
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 11 No. 2 (2023): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v11i2.7904

Pyometra is one of the important causes of infertility in female animals, including dogs. Pyometra is accumulation of pus in the uterus caused by bacteria that are normally in the uterus but in certain circumstances become pathogens due to hormonal influences. This case report aims to determine the etiology, clinical symptoms, diagnosis, prognosis and treatment of pyometra disease in dogs. The examination method that is carried out is physical examination and investigations, hematology, blood chemistry, and ultrasound. Based on a physical examination in the form of inspection of the lesions on the vulva and abdominal enlargement. Palpation of the mesogastrium and hypogastrium is the presence of abdominal tension. Hematologic results show leukocytosis, neutrophilia, and hyperchromic normocytic anemia. Blood chemistry examination results showed azotemia, increased AST, ALP and GGT. Ultrasound examination results show that an anechoic period is accumulation of uterine fluid and thickening of the uterine wall. The diagnosis of this case is pyometra. The therapy performed is ovariohisterectomy surgery. After surgery animals were given medicines including Cephalexine, Meloxicam, Ursodeoxycholic acid, and S-adenosylmethionine (SAMe). On the sixth day the animal surgery post improves again and is allowed to be taken home.

Case report: Demodicosis in a Chow-chow dog at Yours Veterinary Clinic in Padang Panjang City Ridho Gelagar, Ario; Sukmawati, Yofa; Ramadhan, Juliadi; Aulia, Usma; Zukiaturrahmah, Anna
Basic and Applied Nursing Research Journal Vol 4 No 2 (2023): Basic and Applied Nursing Research Journal (BANRJ)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/banrj.04.02.07

Demodecosis, also known as Red Mange, Acarus Mange, and Follicular Mange, is a skin disease caused by the mite Demodex canis. Demodecosis infection can occur in dogs of all ages. This study aims to diagnose and treat the infection of Demodex sp in chow-chow dogs. Clinical signs include generalized alopecia, scales, and crusts found on the body's surface, followed by dehydration symptoms, poor skin turgor, and long CRT. Supporting examinations to determine demodecosis infection are carried out using the deep skin scrapping test method. Next, a hematological exam was carried out. The hematology examination interpreted the blood analysis results to indicate that the dog had normochromic microcytic anemia. A decrease in RBC, hemoglobin, and hematocrit indicates anemia. A low MCV indicates Microcytic, and normochromic is due to a normal MCHC. Treatment is carried out by administering ivermectin, diphenhydramine HCl, and longamox.

Enucleation of Bulbus Oculi in Domestic Cats Patients with Orbital Tumors Ramadhan, Juliadi; Nafis S, Muhammad; Ridho Gelagar, Ario; Aulia, Usma; Zukiaturrahmah, Anna; Sukmawati, Yofa
Basic and Applied Nursing Research Journal Vol 4 No 2 (2023): Basic and Applied Nursing Research Journal (BANRJ)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/banrj.04.02.06

The eye is an essential organ for living things, as one of the five senses of the eye is very vulnerable to infectious or non-infectious diseases. The orbital tumor is one of the disorders in the cat's eye that can attack the eye muscles, nerves, and lacrimal glands. This study aims to diagnose and treat the enucleation of bulbus oculi in domestic cats and patients with orbital tumors. This activity was carried out at the Veterinary Practice "Drh. Juliadi Ramadhan, M.Si." Jl. Payakumbuh-Lintau No. 12 Regency 50 City, West Sumatra Province. The results showed that one of the treatments for orbital tumor cases can be done by enucleation of the oculi bulk. This operation uses a subconjunctival method to remove the eye, the nictitating membrane, and a little eyelid by minimizing the removal of extraocular muscle tissue. After surgery, intense treatment is carried out by maintaining the sanitation of the cage and administering anti-inflammatory drugs, antibiotics, and vitamins.

Edukasi Manfaat Sayur Bagi Kesehatan Anak di Puskesmas Pembantu Bungo Tanjung Tahun 2024 Adzkia, Legabina; Fadhlya Adri, Rantih; Setiana, Iffa; Adi Putri, Silvia; Soni Redha, Pratiwi; Erpidawati, Erpidawati; Susanti, Elsi; Aulia, Usma; Hamdi, Muhammad; Refindra Wijaya, Tiara; Fadila, Nailatul; Latifa Putri, Tasya; Safitri, Rani
Menara Pengabdian Vol 4, No 1 (2024): Vol. 4 No. 1 Juni 2024
Publisher : LPPM Universitas Muhammadiyah Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/jmp.v4i1.5600

Pengembangan media pembelajaran dan materi ajar SMK telah menjadi tuntutan yang urgen setiap jurusan, tuntutan tersebut didasarkan pada perbedaan kebutuhan dan diikuti dengan kemajuan teknologi yang sangat pesat, sehingga menjadi Dwi kebutuhan yang mendesak untuk mengembangkan media dan sumber belajar yang trend. Tujuan pelatihan ini adalah sebagai sarana untuk memberikan pengetahuan bagaimana cara mengembangkan media dan materi ajar yang sesuai dengan kebutuhan peserta didik. Pelatihan ini diawali dengan pengisian form kebutuhan guru terhadap pembelajaran, dilanjutkan dengan seminar pentingnya rancangan media dan materi ajar dan apa saja aplikasi yang dapat digunakan dalam merancang media dan materi ajar, kegiatan ini diakhiri dengan post-test sebagai alat evaluasi dalam proses pelatihan. Hasil pelatihan tersebut dapat disimpulkan bahwa pengguaan aplikasi dan media sosial seperti kahoot, chatGPT dan google form dapat dijadikan alat bantu dalam mengajar yang menarik. Rekomendasi pelatihan ini sangat diperlukan pelatihan tindak lanjut terhadap verifikasi produk yang telah dirancang berbasis teknologi.

EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Aulia, Usma; Wahyuni, Sri; Gholib, Gholib; Dasrul, Dasrul; Adam, Mulyadi; Rahmi, Erdiansyah; Hamzah, Abdullah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.30042

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.

DIAGNOSIS OF MASTITIS IN ETAWA CROSSBREED GOATS Usma Aulia; Rikamalia; Anna Zukiaturrahmah; Juliadi Ramadhan; Ario Ridho Gelagar
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p12

Mastitis is a disease which occur as result of the infection in mammary gland. Mastitis in Etawa Crossbred goat is commonly found and caused economic loss. Subclinical mastitis causes decrease in milk production and milk quality. Subclinical mastitis is a type of mastitis which does not show clinical symptoms. The purpose of this study was to diagnose subclinical mastitis on Etawa Crossbred goat by using the IPB-1 test. This study was a descriptive research. Detection was done by taking milk samples on Etawa Crossbred goat purposively and was reacted with IPB-1 reagents which were circulated circularly for 15-20 seconds. The observation of the reaction results of milk and reagent were determined based on consistency changing with the coagulation period. In this case, blood tests were also carried out. The results showed that Etawa Crossbreed goats was detected with subclinical mastitis with a score of +2 using the IPB-1 test method. The results of blood tests showed that the goat had hyperchromic macrocytic anemia, leukocytosis and monocytosis. Based on the results of the anamnesis, physical examination, and laboratory results, the goat was found to have subclinical mastitis with a fausta prognosis. It is hoped that a thorough examination and the use of different methods can identify the cause of subclinical mastitis in PE goats and facilitate the treatment.

HEMORRHAGIC GASTROENTERITIS IN DOG Anna Zukiaturrahmah; Ario Ridho Gelagar; Usma Aulia; Juliadi Ramadhan
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p40

Haemorrhagic gastroenteritis is a life-threatening disease of dogs. Gastroenteritis is inflammation of the surface of the stomach and intestines which usually occurs due to infection with microorganisms or due to consuming toxic chemicals or certain drugs. This case report aims to determine the etiology, clinical symptoms, diagnosis, prognosis and treatment of Hemorrhagic gastroenteritis disease in dogs. The examination method that is carried out is physical examination and investigations, native stool test, haematology, blood chemistry, X-ray and ultrasound. Based on the anamnesis, it was found that the dog experienced vomiting and bloody diarrhoea. Next, the native feces were examined for the presence of amoeba and bacteria, and there were no parasites. Hematologic results show leucocytosis, neutrophilia, anemia and thrombocytopenia. Blood chemistry examination results showed increased Urea, AST and ALP, and decreased albumin. Xray examination results showed presence of gas in the intestine. The results of the ultrasound examination showed that Bruno's dog's intestines had a hyperechoic appearance. The diagnosis of this case is Haemorrhagic gastroenteritis. Treatment in this case is antibiotics, probiotics, supplements, Vitamin-K, and fluid therapy. On the seventh day the animal is allowed to be taken home. The conclusion of the dog in this case was diagnosed with haemorrhagic gastroenteritis seen from the results of physical examination, supporting examinations in the form of native feces, CBC, blood chemistry, X-Ray and USG. The actions taken were administration of antibiotics, prebiotics, supplements, vit-K, and fluid therapy. It is recommended to improve sanitation of dog eating & drinking equipment and the surrounding environment. Preventive measures can be taken with complete vaccination which can be started in dogs aged six weeks.

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