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Prolactin promoter gene as marker assisted selection (MAS) for the control of broodiness of Kampung chicken Sartika, Tike; Mansjoer, S.S; Saefuddin, A; Martojo, H
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

Preliminary research about MAS (Marker Assisted Selection) was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0) each 24 samples, and from selected population (G3) each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL) chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR). PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation. Â Key words: Kampung chicken, prolactin promoter, MAS

Satin and their crossbred rabbit production Brahmantiyo, Bram; Raharjo, Y.C; Martojo, H; Mansjoer, S.S
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Rabbit productivity in intensive management to be evaluated considering development in the rabbit farming were rapidly increased. Rex, Satin and their crossbred (Reza) rabbits have been developed in Research Institute for Animal Production and productivity information of each breed can be used as basis for policy or model of cultivation and breeding in the community. Research conducted by evaluating the productivity of growth, carcass and the carcass proportion of the three strains of rabbit. Rex and satin growth were no different, and Reza was higher at 12-14 weeks of age. Carcass production comes from cutting the rabbit at the age of six months, this trait is strongly influenced by the weight of rabbits, and Reza gives the lowest performance compared to Rex and Satin. Rex, Satin and Reza were medium type rabbits with dual-purpose product (fur and meat). These rabbits showed the childs growth is good enough, to adapt the environment temperature and high food and have adequate carcass production. Key Words: Rabbit, Rex, Satin, Reza, Growth, Carcass

Genetic diversity of native chicken based on analysis of D-Loop mtDNA marker Sartika, Tike; Duryadi, D; Mansjoer, S.S; Gunawan, B
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

Production was carried out using control region/D-loop mtDNA marker. The base population of native chicken was selected from subpopulation at Cianjur, Jatiwangi, Depok, Bogor I, and Bogor 2. Samples from each population was 10 heads and 2 samples Green Jungle Fowl (Gallus various) from East Java as out Group samples. Two primers binding conserved tRNA Phenylalanine gene and tRNA Glutamine gene were DNA Heavy stranded HI255 (5-CATCTTGGCATCTTCAGTGCC-3) and DNA Light stranded Ll6750 (5-AGGACTACGGCTTGAAAAGC-3) was used to amplify D-Ioop mtDNA chicken. PCR-RFLP methods with 6 restriction enzymes 4 cutter such as, Alul (AGâCT), Hpall (CâCGG), Mbol (âGATC), Rsal (GTâAC), NlaIII (CATGâ) and HaeIII (GGâCC) were used to detect polymorphism within and between subpopulation. Result of experiment show that mtDNA which was amplified by PCR was 1320 bp, consist of 1227 bp control region/D-loop, 45 bp tRNA Glutamine gene and 48 bp tRNA Phenylalananine gene. PCR product which were digested from 6 endonucleases enzyme show that native chicken within and between population was monomorphic and if its compare with Green Jungle Fowl was polymorphic. Â Key words: Native chicken, genetic diversity, mtDNA

Satin and their crossbred rabbit production Bram Brahmantiyo; Y.C Raharjo; H Martojo; S.S Mansjoer
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rabbit productivity in intensive management to be evaluated considering development in the rabbit farming were rapidly increased. Rex, Satin and their crossbred (Reza) rabbits have been developed in Research Institute for Animal Production and productivity information of each breed can be used as basis for policy or model of cultivation and breeding in the community. Research conducted by evaluating the productivity of growth, carcass and the carcass proportion of the three strains of rabbit. Rex and satin growth were no different, and Reza was higher at 12-14 weeks of age. Carcass production comes from cutting the rabbit at the age of six months, this trait is strongly influenced by the weight of rabbits, and Reza gives the lowest performance compared to Rex and Satin. Rex, Satin and Reza were medium type rabbits with dual-purpose product (fur and meat). These rabbits showed the child's growth is good enough, to adapt the environment temperature and high food and have adequate carcass production. Key Words: Rabbit, Rex, Satin, Reza, Growth, Carcass

Genetic diversity of native chicken based on analysis of D-Loop mtDNA marker Tike Sartika; D Duryadi; S.S Mansjoer; B Gunawan
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Production was carried out using control region/D-loop mtDNA marker. The base population of native chicken was selected from subpopulation at Cianjur, Jatiwangi, Depok, Bogor I, and Bogor 2. Samples from each population was 10 heads and 2 samples Green Jungle Fowl (Gallus various) from East Java as out Group samples. Two primers binding conserved tRNA Phenylalanine gene and tRNA Glutamine gene were DNA Heavy stranded HI255 (5'-CATCTTGGCATCTTCAGTGCC-3') and DNA Light stranded Ll6750 (5'-AGGACTACGGCTTGAAAAGC-3') was used to amplify D-Ioop mtDNA chicken. PCR-RFLP methods with 6 restriction enzymes 4 cutter such as, Alul (AG↓CT), Hpall (C↓CGG), Mbol (↓GATC), Rsal (GT↓AC), NlaIII (CATG↓) and HaeIII (GG↓CC) were used to detect polymorphism within and between subpopulation. Result of experiment show that mtDNA which was amplified by PCR was 1320 bp, consist of 1227 bp control region/D-loop, 45 bp tRNA Glutamine gene and 48 bp tRNA Phenylalananine gene. PCR product which were digested from 6 endonucleases enzyme show that native chicken within and between population was monomorphic and if its compare with Green Jungle Fowl was polymorphic. Key words: Native chicken, genetic diversity, mtDNA

Prolactin promoter gene as marker assisted selection (MAS) for the control of broodiness of Kampung chicken Tike Sartika; S.S Mansjoer; A Saefuddin; H Martojo
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Preliminary research about MAS (Marker Assisted Selection) was conducted to detect broodiness trait of Kampung chicken. MAS currently is very important in situations, where the accuracy of selection is low, such as, traits with low heritability, e.g. broodiness trait and egg production. Prolactin promoter was selected as a marker gene for broodiness because it plays a critical part in the neuroendocrine cascade which is triggered at the onset of broodiness. DNA samples were collected from low and highbroodiness samples on basic population (G0) each 24 samples, and from selected population (G3) each 28 samples. As control population without broody behavior was used 16 samples White Leghorn (WL) chicken. Prolactin promoter gene was amplified using polymerase chain reaction (PCR). PCR product was analyzed using electrophoresis agarose gel 2%. The results showed four types of bands represent in the Kampung chicken, three types called as wild type band and one type as the WL band. The chickens with low and high broodiness on G0 generation have 75 and 87.5% of wild type band while in the G3 generation was decreased to 25 and 75%. Conclusions of the research indicated that the selected breed of the Kampung chicken on G3 generation increased WL band like White Leghorn chicken as much as 31,25% from the G0 generation. Key words: Kampung chicken, prolactin promoter, MAS

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