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INDRA RAHMAWATI
Center for Biotechnology , BPPT, Puspiptek-Serpong, Tangerang Selatan, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Isolation and Identification of a Thermostable Amylase-Producing Bacterium from Hatuasa Hotspring DOMINGGUS MALLE; JUNUS PICARIMA; LAURY CHARA HUWAE; INDRA RAHMAWATI; WAHYU PURBOWASITO
Microbiology Indonesia Vol. 6 No. 2 (2012): June 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1801.672 KB) | DOI: 10.5454/mi.6.2.5

Abstract

Hot springs are a common source of thermophyles which produce thermostable enzymes. The objective of this study was to isolate and identify thermostable amylase-producing bacteria from a local geothermal spring. An amylase–producing bacterium strain was isolated from this hot spring which excreted amylase after being grown on starch agar screening plates at 37 °C. It was identified as Bacillus megaterium using the method of 16S ribosomal DNA. The organism is a rod-shape and is a spore-forming  bacterium.  Maximum amylase production was achieved after incubation in the production media for 72 hours. Preliminary analysis of the secreted amylase showed that the enzyme could bind to DEAE-Sepharose matrix and was discharged by eluting with 0.5 M NaCl. The partially purified enzyme was stable up to 75 °C, showing that this enzyme might have potential application in the starch-processing industry.
Co-Authors DOMINGGUS MALLE JUNUS PICARIMA LAURY CHARA HUWAE WAHYU PURBOWASITO