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All Journal Microbiology Indonesia Indonesian Journal of Rheumatology
EDHYANA SAHIRATMADJA
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Cloning, Expression and Bioinformatic Analysis of Human Papillomavirus Type 52 L1 Capsid Gene from Indonesian Patient SONY SUHANDONO; DEWI AYU KENCANA UNGU; TATI KRISTIANTI; EDHYANA SAHIRATMADJA; HERMAN SUSANTO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.271 KB) | DOI: 10.5454/mi.8.3.2

Abstract

Human papillomavirus (HPV) type 52 is the most prevalent type for causing cervical cancer in Indonesian population. Cervical cancer becomes the most common cancer suffered by Indonesian women. Prevention of HPV infection can be achieved using HPV virus-like particle (VLP) vaccine derived from L1 major capsid protein.  This study aimed to clone and analyze HPV-52 L1 gene. DNA obtained from biopsy of a cervical cancer patient was amplified using specific primers designed from Asian originated HPV-52 L1 gene available in the GenBank. The isolated HPV-52 L1 gene sequence was submitted to GenBank with accession number [KF225497]. Expression of HPV-52 L1 gene was performed using pRSET/EmGFPEscherichia coli expression vector. We analyzed and compared the HPV-52 L1 gene expressions from recombinant E.coli BL21 (DE3) that had been induced for 3 hours with 1 mM IPTG and without induction. The protein was expressed in insoluble form. We performed the following bioinformatic analyses: construction of phlyogenetic tree, T-cell epitopes prediction and 3D proteins structure modelling. We utilized the following softwares: MEGA5 for phylogenetic tree, IEDBann for MHC prediction, CLC DNA Workbench 6.5 for hydrophobicity analysis and PDB-Viewer Deep for 3D protein structure analysis. The phylogenetic tree which was developed based on [KF225497] sequence showed that it shared a branch with Asian countries (Philippines and Thailand). The deduced amino acid sequences of the predicted epitopes that were consistent in all of the programs were 259GTLGDPVPGDLYIQGS274 and 345KKESTYKNE353. This information may be useful to design diagnostic strategies and vaccine suitable for Indonesian population.
Early Detection Of Thalassemia Carrier In Patients With SystemicLupus Erythematous: The Use of Shine and Lal Index Edhyana Sahiratmadja
Indonesian Journal of Rheumatology Vol. 17 No. 1 (2025): IJR VOL 17 No 1
Publisher : Indonesian Rheumatology Associantion

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ijr.v17i1.16

Abstract

Background: Anemia in systemic lupus erythematous (SLE) is common due toimpaired erythropoietin response and the presence of antibodies that attackerythropoietin. Various anemia in SLE include autoimmune hemolytic anemia (AIHA),anemia of chronic disease (ACD), or iron deficiency anemia (IDA). Interestingly,Indonesia lies in thalassemia belt area and it is estimated to harboring around 6-10%thalassemia carrier in its population. Therefore, anemia in SLE patients should befurther examined for its possibility for thalassemia carrier. This study aimed todetermine whether a simple erythrocyte index could be used for thalassemia carrierearly detection of SLE patients living in Indonesia. Methods: This study had aretrospective and cross-sectional design, collecting hematology data of SLE patientsregistered at Dr. Hasan Sadikin General Hospital. Erythrocyte indices, includingMentzer index (MCV/RBC) and Shine & Lal index (MCV.MCH.MCH/100), wereassessed to determine IDA or thalassemia carrier. Results: Of 259 hematology datafrom SLE patients included, predominantly female (95.8%), aged 34 years old (median;range 17-65 years) and single (27%), of whom 45.2% had anemia. However, most ofthem were anemia normocytic or normochromic. Interestingly, Mentzer index (<13) wasfound in 2.7% and Shine & Lal index (<1530) in 10% patients, suggesting considerthalassemia carrier. Conclusion: Although Shine & Lal index has lower sensitivitycompared with Mentzer index, Shine & Lal Index might serve a broader screening toolas an early detection for thalassemia carrier.  
Co-Authors DEWI AYU KENCANA UNGU Herman Susanto SONY SUHANDONO TATI KRISTIANTI