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THE CONCEPTUAL CHANGE ASSESSMENT BASED ON ESSAY QUESTIONS IN CASE STUDY OF DNA/RNA AND INTRON TOPICS Kristianti, Tati; Widodo, Ari; Suhandono, Sony
Jurnal Penelitian Pendidikan IPA Vol 4, No 1 (2019): Juni 2019
Publisher : Perkumpulan Pendidik IPA Indonesia (PPII)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jppipa.v4n1.p31-37

Most of the study on conceptual change was analysed using multiple-choice, true/false and true/false-reason type of questions. However, these questions are unable to reveal the variation in understanding of the university students. In this study, we developed a new conceptual change assessment based on essay questions. Here, student responses on DNA and intron term are presented as case studies in order to show the application of this assessment. The assessment is able to classify the various degree of understanding in university students such as construction, revision, complementation, static and disorientation. Our findings in university students studying DNA term showed that of 70 university students, 34% (construction), 36% (revision), 21% (complementation), 4% (static) and 5% (disorientation). On intron term finds construction (4%), revision (14%), static (44%) and disorientation (38%). Overall analysis using various categories of understanding reveals that DNA term is easier to understand by the university students than the intron term. This assessment is useful to evaluate the conceptual change level of university students in the class. Therefore, the assessment might also be useful to evaluate various teaching strategies and other topics.Keywords: conceptual change, essay, assessment, DNA, Intron

Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.) Iskandar, Hayati Minarsih; Suhandono, Sonny; Pambudi, Jembar; Kristianti, Tati; Putranto, Riza Arief; Mose, Windi; Sustiprijatno, Sustiprijatno
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Dehydrin (DHN) is known to play an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Previous research reported the increased expression of DHN in sugarcane stems exposed to drought stress for 15 days which may be controlled by its corresponding stress inducible promoter. The DHN promoter was succesfully isolated from sugarcane variety PSJT 941 (Pr-1DHNSo) and was cloned to pBI121 expression vector fused to a β-glucuronidase (GUS) reporter gene. The aim of this research was the functional testing of the Pr-1DHNSo promoter through transformation into tobacco plant treated with in vitro drought stress. Genetic transformation of Pr-1DHNSo construct was conducted by Agrobacterium tumefaciens. The transformed tobacco was then subjected to drought stress treatment using 40% PEG 6000 for five sequential incubations (0, 12, 24, 48 and 72 hours). The GUS assay reveal that the transformed tobacco treated with drought stress showed a blue color denoting GUS activity in leaf, stem and root tissues and this expression increased along with the length of the drought treatment. The analysis of gusA gene using real time-qPCR normalized to the L25 reference gene also showed that the expression increased in line with the length of time of drought stress. The results presented in this study indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco.

THE CONCEPTUAL CHANGE ASSESSMENT BASED ON ESSAY QUESTIONS IN CASE STUDY OF DNA/RNA AND INTRON TOPICS Kristianti, Tati; Widodo, Ari; Suhandono, Sony
Jurnal Penelitian Pendidikan IPA Vol 4, No 1 (2019): Juni 2019
Publisher : Perkumpulan Pendidik IPA Indonesia (PPII)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jppipa.v4n1.p31-37

Most of the study on conceptual change was analysed using multiple-choice, true/false and true/false-reason type of questions. However, these questions are unable to reveal the variation in understanding of the university students. In this study, we developed a new conceptual change assessment based on essay questions. Here, student responses on DNA and intron term are presented as case studies in order to show the application of this assessment. The assessment is able to classify the various degree of understanding in university students such as construction, revision, complementation, static and disorientation. Our findings in university students studying DNA term showed that of 70 university students, 34% (construction), 36% (revision), 21% (complementation), 4% (static) and 5% (disorientation). On intron term finds construction (4%), revision (14%), static (44%) and disorientation (38%). Overall analysis using various categories of understanding reveals that DNA term is easier to understand by the university students than the intron term. This assessment is useful to evaluate the conceptual change level of university students in the class. Therefore, the assessment might also be useful to evaluate various teaching strategies and other topics.Keywords: conceptual change, essay, assessment, DNA, Intron

KARAKTERISASI PRODUKSI RHAMNOLIPID MENGGUNAKAN PENDEKATAN BIOLOGI SINTETIK Nina Imaniar; Tati Kristianti; Sony Suhandono
Jurnal Farmamedika (Pharmamedika Journal) Vol 6 No 1 (2021): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v6i1.125

Ketersediaan cadangan minyak mentah yang minim di Indonesia menjadi dasar dari penelitian ini untuk membuat sebuah inovasi baru yang dapat diterapkan dalam teknik enhanced oil recovery menggunakan agent biologi rekombinan. Agen tersebut di desain sebagai penghasil rhamnolipid yang dapat meningkatkan volume ekstraksi minyak mentah dari dalam kilang minyak. Tujuan penelitian ini adalah mengkarakterisasi rhamnolipid rekombinan yang dihasilkan melalui pendekatan biologi sintetik Gen pengkode Rhamnolipid (RhlAB) di insersikan ke dalam pUC57 dibawah kontrol promoter T7. Hasil analisis kuantitatif menunjukan konsentrasi rhamnolipid tertinggi di peroleh setelah inkubasi selama 15 jam di dalam medium pertumbuhan Luria Bertani dengan pH7 dan temperature pertumbuhan 37°C, sedangkan analisis kualitatif menunjukan bahwa rhamnolipid rekombinan juga memiliki kemampuan untuk menurunkan tegangan permukaan dan memiliki aktivitas drop collapse. Kesimpulan penelitian ini adalah metode biologi sintetik dapat digunakan untuk memproduksi rhamnolipid rekombinan dengan aktivitas serupa dengan rhamnolipid yang di hasilkan dari species aslinya.

Peningkatan Mutu Guru Ipa Melalui Pelatihan Penggunaan Alat Analisis Kolorimetri Sederhana Berbasis Pencitraan Digital dengan Menggunakan Scanner Chevi Ardiana; Lida Amalia; Sri Mulyaningsih; Tati Kristianti; Dewi Hernawati
Jurnal Pekemas Vol 2 No 1 (2019): Jurnal Pekemas Tahun 2019
Publisher : Lembaga Pengabdian kepada Masyarakat Institut Pendidikan Indonesia Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar

Colorimetry is a quantitative analysis technique for colored samples, which is used to determine composition (concentration). Technological advancements led to the discovery of modern (instrumentation) tools to complete the colorimetric analysis received by UV-VIS spectrophotometers especially in SMA / MA schools. UV-Vis Spectrophotometers are rarely owned because they are expensive. The impact is limited practicum activities in SMA / MA schools even though practical activities are needed to provide proof of a theory. Therefore, alternative laboratory tools are needed to overcome this problem, including a simple digital imaging based colorimetric analysis tool using a scanner. In order for this alternative tool to be known in the school environment, socialization is needed, namely by training and simulation. The training method used to explain the concept of digital imaging-based colorimetric analysis is supported by demonstration examples of determining the content (concentration) of a substance in the sample. This method is used as training in the operation of digital imaging techniques by using a scanner to obtain images from the sample. Simulation method is used to indicate a software application that can be used to change the image of the imaging results into light intensity (I) then convert to light absorption value (absorbance). It can be concluded that the digital imaging technique using a scanner can be used as an alternative tool to replace UV-Vis spectrophotometer in determining the content (concentration) of substances in the sample and as a support for science / Biology / Chemistry learning in schools. The response of the participants who participated in this activity was very good and they were motivated to apply it in school

Pelatihan Pembuatan Hand Sanitizer Untuk Guru SMPN 1 Banyuresmi Garut Pada Masa Adaptasi Kebiasaan Baru (AKB) Indra Dodo Saputra; Leni Sri Mulyani; Chevi Ardiana; Tati Kristianti
Jurnal Pekemas Vol 3 No 2 (2020): Jurnal Pekemas Tahun 2020
Publisher : Lembaga Pengabdian kepada Masyarakat Institut Pendidikan Indonesia Garut

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Health is a very important aspect of life. One of ways to maintain it by taking care of your hands. A Hand sanitizer (antiseptic tangan) is a health’s product that can instantly kill microorganisms without using water and it can be used anytime and anywhere. The purpose of these dedications realizes one of the Tri Dharma colleges, socializes Clean and Healthy Living habits (PHBS) and it can produce a hand sanitizer that can be used properly for oneself, families, educational institutions and the surrounding societies. The method uses explanatory material and the practice of making hand sanitizers. The data analysis technique uses descriptive. The result of this dedications are the participants' understanding of the manufacture of hand sanitizer, the functions of hand sanitizer and the application of clean and healthy living habits

Isolation and characterization of Dehydrin promoter region from sugarcane (Saccharum officinarum L.) Hayati MINARSIH; Sonny SUHANDONO; Anissa K FUADI; Tati KRISTIANTI; Riza A PUTRANTO; Deden SUKMAJDAYA; . SUSTIPRIJATNO
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli.

Ursodeoxycholic acid: a systematic review on the chemical and biochemical properties, biosynthesis, sources and pharmacological activities Marlin Megalestin Raunsai; Elfahmi; Agus Chahyadi; Sony Suhandono; Tati Kristianti; Laode M.R. Al Muqarrabun
Current Research on Bioscences and Biotechnology Vol. 3 No. 1 (2021)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2021.3.1/UJZVBNA0

Ursodeoxycholic acid (UDCA), a secondary bile acid (BA), is an acidic steroid synthesized from cholesterol in hepatocytes. UDCA is widely used for the treatment of various diseases related to liver injury. The use of UDCA to treat non-liver diseases has also been developed recently, such as neurodegenerative diseases, cancer, and obesity. Due to the important role of UDCA on human health, numerous studies in understanding its chemical and pharmacological properties have been published. Literature sources were obtained from online databases such as Science Direct, Google Scholar, Scopus and PubMed using keywords relating to the purpose of study. Critical analysis and review were performed for all literature. UDCA is a steroid compound with pharmacological properties. Seventeen enzymes are involved in its biosynthesis, which has been proposed in four pathways: classic, alternative, the Yamazaki, and 25-hydroxylation pathways. UDCA can be isolated from bovine bile, bear bile or all Ursidae, human, rabbit, cow, rat, hamster, sheep, pig, and plant. UDCA has been used in the treatment of several diseases such as primary biliary cirrhosis, intrahepatic cholestasis of pregnancy, hepatolithiasis associated with Caroli syndrome gallstones, cystic fibrosis, hepatitis C virus, chronic heart failure, neurodegenerative diseases, and obesity, as well as in the prevention of cancer. UDCA has a wide range of pharmacological properties. Further investigations on its efficacy and safety on humans are required before it could be used for several indications. All genes which are responsible in UDCA biosynthesis have been elucidated. That said, further genetic engineering studies in order to find other prospective sources of UDCA could be a challenge for the future research.

THE CONCEPTUAL CHANGE ASSESSMENT BASED ON ESSAY QUESTIONS IN CASE STUDY OF DNA/RNA AND INTRON TOPICS Tati Kristianti; Ari Widodo; Sony Suhandono
Jurnal Penelitian Pendidikan IPA Vol. 4 No. 1 (2019): Juni 2019
Publisher : Perkumpulan Pendidik IPA Indonesia (PPII)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jppipa.v4n1.p31-37

Most of the study on conceptual change was analysed using multiple-choice, true/false and true/false-reason type of questions. However, these questions are unable to reveal the variation in understanding of the university students. In this study, we developed a new conceptual change assessment based on essay questions. Here, student responses on DNA and intron term are presented as case studies in order to show the application of this assessment. The assessment is able to classify the various degree of understanding in university students such as construction, revision, complementation, static and disorientation. Our findings in university students studying DNA term showed that of 70 university students, 34% (construction), 36% (revision), 21% (complementation), 4% (static) and 5% (disorientation). On intron term finds construction (4%), revision (14%), static (44%) and disorientation (38%). Overall analysis using various categories of understanding reveals that DNA term is easier to understand by the university students than the intron term. This assessment is useful to evaluate the conceptual change level of university students in the class. Therefore, the assessment might also be useful to evaluate various teaching strategies and other topics.Keywords: conceptual change, essay, assessment, DNA, Intron

Cloning, Expression and Bioinformatic Analysis of Human Papillomavirus Type 52 L1 Capsid Gene from Indonesian Patient SONY SUHANDONO; DEWI AYU KENCANA UNGU; TATI KRISTIANTI; EDHYANA SAHIRATMADJA; HERMAN SUSANTO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Human papillomavirus (HPV) type 52 is the most prevalent type for causing cervical cancer in Indonesian population. Cervical cancer becomes the most common cancer suffered by Indonesian women. Prevention of HPV infection can be achieved using HPV virus-like particle (VLP) vaccine derived from L1 major capsid protein. Â This study aimed to clone and analyze HPV-52 L1 gene. DNA obtained from biopsy of a cervical cancer patient was amplified using specific primers designed from Asian originated HPV-52 L1 gene available in the GenBank. The isolated HPV-52 L1 gene sequence was submitted to GenBank with accession number [KF225497]. Expression of HPV-52 L1 gene was performed using pRSET/EmGFPEscherichia coli expression vector. We analyzed and compared the HPV-52 L1 gene expressions from recombinant E.coli BL21 (DE3) that had been induced for 3 hours with 1 mM IPTG and without induction. The protein was expressed in insoluble form. We performed the following bioinformatic analyses: construction of phlyogenetic tree, T-cell epitopes prediction and 3D proteins structure modelling. We utilized the following softwares: MEGA5 for phylogenetic tree, IEDBann for MHC prediction, CLC DNA Workbench 6.5 for hydrophobicity analysis and PDB-Viewer Deep for 3D protein structure analysis. The phylogenetic tree which was developed based on [KF225497] sequence showed that it shared a branch with Asian countries (Philippines and Thailand). The deduced amino acid sequences of the predicted epitopes that were consistent in all of the programs were 259GTLGDPVPGDLYIQGS274 and 345KKESTYKNE353. This information may be useful to design diagnostic strategies and vaccine suitable for Indonesian population.

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