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All Journal Microbiology Indonesia Digital Bisnis: Jurnal Publikasi Ilmu Manajemen Dan E-commerce
SILMI RAHMANI
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Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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The Potency of Aluminum Hydroxide Nanoparticles for Dengue Subunit Vaccine Adjuvant SABAR PAMBUDI; ETIK MARDLIYATI; SILMI RAHMANI; DAMAI RIA SETYAWATI; TIKA WIDAYANTI; ANGELINA GILL; ASRI SULFIANTI; WHINIE LESTARI
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (984.8 KB) | DOI: 10.5454/mi.12.3.5

Abstract

The potency of aluminum hydroxide as an adjuvant in vaccine development is considered to depend on its particle size. In previous studies, we have successfully prepared two size particle, micro, and nano, aluminum hydroxide gel (alum) adjuvants. The potency of those particles as a candidate of adjuvant is needed to be characterized. In this study, we formulated our adjuvants with purified DENV3 pre Membrane Envelope (prM-E) recombinant protein and evaluated the induction of nitric oxide level in mouse macrophage RAW 264.7 cells. We prepared the alum adjuvant by precipitation-homogenization methods with an agitation rate at 11,000xg. Secreted prM-E recombinant protein was collected from Pichia pastoris X-33 fermentation which produced using bioreactor. Recombinant protein purification was carried out by anion exchange chromatography followed with size exclusion chromatography. The purified prM-E recombinant protein was observed as a single band around 70 -1k Da with a concentration of 105 mg mL . Complex nanoparticles alum with prM-E protein significantly (p<0.05) induced the nitric oxide level. Further analysis should be conducted in order to discover the detail molecular mechanism of nanoparticle alum adjuvant, recombinant protein, and cellular immune response.
Penerapan Technology Academy Dalam Meningkatkan Soft Skill Melalui Program Studi Independen Kampus Merdeka Pada PT Revolusi Citra Edukasi (Revou) Silmi Rahmani; Jojok Dwiridotjahjono
Digital Bisnis: Jurnal Publikasi Ilmu Manajemen dan E-Commerce Vol. 2 No. 3 (2023): September : Digital Bisnis : Jurnal Publikasi Ilmu Manajemen dan E-Commerce
Publisher : Universitas 45 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30640/digital.v2i3.1317

Abstract

The Merdeka Campus Program is a program organized by the Ministry of Education, Culture, Research and Technology of the Republic of Indonesia, one of the programs is the Independent Study in partnership with PT Revolusi Citra Edukasi which is called RevoU Technology, this program has the aim that the participants gain experience and knowledge about technology academy which is useful for the world of work, by participating in an independent study program the participants will meet various students throughout Indonesia as well as mentors who are experienced in their fields, participants will also know the impact of implementing technology academy on companies such as the impact of product management, digital marketing , data analyst and software engineering
Co-Authors ANGELINA GILL ASRI SULFIANTI DAMAI RIA SETYAWATI Etik Mardliyati Jojok Dwiridotjahjono Sabar Pambudi TIKA WIDAYANTI Whinie Lestari