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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science
Kurniawan Budiarto
Indonesian Ornamental Crops Research Institute
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Elimination Of CVB (Chrysanthemum Virus B) From A Range Of ChrysanthemumTHE Varieties By Meristem Culture Following Antiviral and Heat Treatments Kurniawan Budiarto; Budi Marwoto Budi; Lia Sanjaya Lia; Muchdar Soedarjo Muchdar; Indijarto Budi Rahardjo Indijarto
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 18 No. 2 (2011)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.283 KB) | DOI: 10.11598/btb.2011.18.2.45

Abstract

CVB elimination for retaining healthy protocols from infected chrysanthemum plant was investigated trough combined treatment of meristem culture with synthetic antiviral ribavirin and thermotherapy under in vitro conditions. The biological material used on the experimentation was constituted of six commercial varieties: Dewi Sartika, Saraswati, Yellow Fiji, White Puma, Yellow Puma and White Reagent.  Tissue culture initiation was conducted through plantlet establishment using MS supplemented with IAA.  Ribavirin was added in media treatments with the concentration of 40 mg/l.  Parallel with this step, heat treatment with different durations (1, 2, and 3 weeks) was also conducted on the plantlets.  Meristem culture was done following the chemo- and thermotherapy.  The experiment resumed the failure single treatment of meristem culture in eliminating CVB from the infected chrysanthemum plantlets.  Under heat treatment, percentage of virus-free plantlets increased along with the duration of thermotherapy, though the survival rate of plantlets decreased in lengthened heat treatment. The best results regarding virus free plant percentage was obtained when meristem culture was applied following ribavirin or three weeks heat treatment which effectively eliminated virus particles from the plant.
Evaluation of Biological and Chemical Fungicides to Control White Rust in Chrysanthemum Grown Under Open Condition Indijarto Budi Rahardjo; Kurniawan Budiarto; Budi Marwoto
AGRIVITA, Journal of Agricultural Science Vol 41, No 1 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i1.1787

Abstract

The effort to reduce chemicals has become an important issue in floriculture agribusiness. The chemicals included fungicides for white rust control in chrysanthemum. The combined application of synthetic fungicide with biological agent and botanical fungicide were expected not only to reduce synthetic fungicide application, but also suppressed the white rust attacks. The research was conducted at the Indonesian Ornamental Crops Research Institute (IOCRI). A factorial randomized complete blocked experiment was designed to facilitate combined applications of hexaconazol-50 (SFH-50), antagonist microbe Corynebacterium sp. (Cor-5) and formulated botanical fungicide CEES 50EC (ES-50). The results showed that the combined SFH-50 with Cor-5 and ES-50 merely affected the disease intensity, yet less on white rust incidence, period of disease emergence, plant growth, flower production and quality. All combined applications of SFH-50 with Cor-5 and ES-50 showed lower disease intensity than single SFH-50. Higher suppressions were detected on 1 ml/l SFH-50 + 5 ml/l Cor-5-3 and 1 ml/l SFH-50 + 2 ml/l ES-50-3. The use of antagonist microbe and botanical fungicide singly or in combination with synthetic fungicide could reduce with the higher affectivity than single synthetic fungicide application.
Co-Authors Budi Marwoto Budi Marwoto Budi Indijarto Budi Rahardjo Indijarto Budi Rahardjo Indijarto Lia Sanjaya Lia Muchdar Soedarjo Muchdar