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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BioEksakta : Jurnal Ilmiah Biologi Unsoed

Oedjijono Oedjijono

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Author-ID : 3157029

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER Stalis Norma Ethica; Oedjijono Oedjijono; Endang Semiarti; Jaka Widada; Tri Joko Raharjo
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.

Isolasi dan Uji Resistensi Bakteri Endofit Eceng Gondok Terhadap Krom Ade Nurmalasari; Oedjijono Oedjijono; Sri Lestari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Endophytic bacteria are bacteria that live in plant tissues and are not pathogenic to the host. One of endophytic bacterial host is water hyacinth. Water hyacinth could accumulate heavy metals, one of which is chrome. The purpose of this study was to obtain endophytic bacteria of water hyacinth (Eichhornia crassipes Mart.), to identify the endophytic bacteria of water hyacinth, and to determine the resistance of endophytic bacteria of water hyacinth to Chrome (Cr). The research method used was a survey with a purposive random sampling technique. Stages of research include root sampling, sterilization of root samples using 2% NaOCl3, isolation of water hyacinth endophytic bacteria on agar nutrient medium (NA), characterization of bacterial endophytes and resistance test of water hyacinth bacteria to chromium. Based on the results of the study, 8 isolates of endophytic bacteria were isolated from the roots of water hyacinth. Based on Bergey's Manual Determinative of Bacteriology, 6 bacterial isolates (E1, E2, E3, E5, E6, E8) were identified as Bacillus and 2 isolates (E4, E7) were identified as Pseudomonas. All water hyacinth endophytic bacteria obtained were resistant to chromium to a concentration of 750 mg/L.

Pengaruh Penambahan Prebiotik Inulin dan Fruktooligosakarida (FOS) terhadap Pertumbuhan Probiotik Bifidobacterium sp. Bb2E Azma Nurizqi Isnasari; Dyah Fitri Kusharyati; Oedjijono Oedjijono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3385

Human health is very closely related to the condition of the human digestive tract since the beginning of life. The diversity of microorganisms found in the human digestive tract is very diverse, consisting of 300-500 different species of bacteria to increase the working power of nutrition. Prebiotics such as inulin and FOS and probiotics such as Bifidobacteria are aspects that can be added for the increase of nutrition. Optimal bacterial growth can be seen in the bacterial growth curve. The research problems were how the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, the amount of incubation time needed to support the growth of Bifidobacterium sp. Bb2E, and how the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. The purposes of this study were to study the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, to know the optimal incubation time of Bifidobacterium sp. Bb2E, and to know the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. This research was conducted using a completely randomized design (CRD) with factorial patterns. The main parameter measured was the population of Bifidobacterium sp. Bb2E, and the additional parameters measured were the pH level and the value of lactic acid titrated. The independent variable discussed in this study is prebiotic estimation on the medium, while the dependent variable considered is the population of Bifidobacterium sp. BB2E. The results of this study showed that the addition of inulin and fructooligosaccharide prebiotic at different incubation times had a significant effect on the growth of Bifidobacterium sp. Bb2E. The best treatment was a combination of inulin + FOS at incubation time of 18 hours with an optical density value was 1,794 and a total population density was 2,44x1010 CFU/mL.

INOKULASI RIZOBAKTERIA PADA TANAMAN CABAI (Capsicum annum L.) DI TANAH SALIN Monica Widi anti; Oedjijono Oedjijono; Elly Proklamasiningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

A red chili plant (Capsicum spp.) is one of the horticultural commodities in Indonesia which has good economic value, so it has priority to be developed. Agriculture practices in Java island have partly switched to coastal areas which have characteristics of saline soils. Saline soils are alkaline and nutrient-poor, especially essential nutrients such as P and N. One way to restore fertility in saline soils used beneficial and fertilizing bacteria such as PGPR. Plant growth-promoting rhizobacteria (PGPR) are free-living, soil-borne bacteria, which are capable of enhancing the growth of the plant either directly or indirectly. The objectives of this research were to determain the effect of PGPR isolates to the growth of red chili plants in saline sandy soil and to determine the best PGPR isolate capable to improve the growth of red chili plant in saline sandy soil. The result showed that the inoculation of rhizobacteria significantly affected to plant height and root length of the red chili plant (Capsicum annum). The combination of 3 PGPR isolates (Azospirillum PSA 10, Azotobacter PSA 8, and Marinococcus PSA 1) was the best result in increasing the height and root length of the chili plant (Capsicum annum).

Eksplorasi Bakteri Diazotrof dari Rizosfer Tanaman Bawah Merah (Allium ascalonicum L.) di Brebes, Jawa Tengah Dwi Ayu Lutfiani Amalia; Oedjijono Oedjijono; Purwanto Purwanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3480

Penelitian ini bertujuan untuk mengeksplorasi bakteri diazotrof yang mampu menambat nitrogen dan menghasilkan hormon IAA dengan cara menyeleksinya dari rizosfer tanaman bawang merah di Brebes, Jawa Tengah. Penelitian dilakukan selama 6 bulan (Januari-Juni 2020). Penelitian ini terdiri dari pengambilan sampel tanah perakaran bawang merah di Brebes, Jawa Tengah, isolasi bakteri tanah pada medium Yeast Mannitol Agar+Congo red, Ashby, dan Caceres, uji produksi IAA metode Salkowski, uji kemampuan penambatan nitrogen metode Kjeldahl, dan identifikasi bakteri. Sebanyak sembilan isolat bakteri diazotrof yang mampu menghasilkan IAA telah berhasil diisolasi. Kesembilan isolat tersebut mampu menghasilkan IAA dengan konstentrasi antara 3,05-3,51 ppm, dengan isolat LAR3 sebagai penghasil IAA tertinggi. Hasil perhitungan kemampuan menambat nitrogen bebas dengan metode Kjeldahl dari 6 isolat terbaik penghasil IAA, mampu menghasilkan konsentrasi berkisar antara 3,15-88,55 ppm, dengan isolat LAR5 sebagai penghasil nitrogen tertinggi. Hasil identifikasi menunjukkan kesembilan isolat bakteri yang didapatkan termasuk dalam 3 kelompok bakteri yang berbeda, yaitu empat isolat termasuk spesies anggota genus Rhizobium (isolat LAR3,LAR5,LBR1,dan LCR3), tiga isolat adalah spesies anggota genus Azospirillum (isolat LAA4,LAA5, dan LCA1), dan dua isolat termasuk spesies anggota genus Azotobacter (isolat LBZ2 dan LBZ3).

Co-Authors Ade Nurmalasari Azma Nurizqi Isnasari Dwi Ayu Lutfiani Amalia Dyah Fitri Kusharyati Elly Proklamasiningsih Endang Semiarti JAKA WIDADA Monica Widi anti Purwanto Purwanto SRI LESTARI Stalis Norma Ethica Tri Joko Raharjo

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