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All Journal Microbiology Indonesia Pro-Life
Isma Nur Azzizah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology

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STUDI PEMANFAATAN TUMBUHAN SEBAGAI BAHAN OBAT OLEH MASYARAKAT DESA MARIMABATE DI KECAMATAN JAILOLO, HALMAHERA BARAT Anisatu Z. Wakhidah; Ika Pratiwi; Isma Nur Azzizah
Jurnal Pro-Life Vol. 4 No. 1 (2017): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

An inventory of plants as medicinal material based on local knowledge in east region Indonesia, especially in Halmahera Island, is lacking. This local knowledge, which is inherited orally from elder to their next generation, will be extinct if the information is not documented. Furthermore, plants utilized without conservation effort may cause extinction. According to these reasons, an investigation about local knowledge in plants utilized as medicinal material by Marimabate villagers on Sub district Jailolo, West Halmahera has been conducted in March 2014. The objective of this study is explaining medicinal plants based on local knowledge of Marimabate community and their conservation behaviour. Ethnobotanical data was collected by using semi-structured interview method to shaman and villagers. From the investigation, 21 species of used plant belonged 14 families were recorded. The data showed that leaf was the most often part used among others, they were 16 species. Almost of the using ways of plant was by mixed with warm water then drank to patients. The cureable diasease were 23, such as ‘lidah putih’, ‘bibit-malaria’, menstruation syndrome, and skin burned. The majority medical plants resource was from semi-wild plant (50% species), that showedthe community conservation behavior has been enough applied. Keywords: medical plants, medical material, Marimabate village, West Halmahera
Cloning and expression of NS2B/NS3 protein of DENV3 Indonesia strain in Saccharomyces cerevisiae expression system for the development of Dengue vaccine ASRI SULFIANTI; SABAR PAMBUDI; ISMA NUR AZIZAH; DODDY IRAWAN SETYO UTOMO; ABINAWANTO ABINAWANTO
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3936.725 KB) | DOI: 10.5454/mi.12.2.4

Abstract

NS3 protein is 618 amino acids (aa) in length containing serine protease and helicase domains required for DENV replication. Alignment of consensus amino acid sequences from all four DENV serotypes demonstrated that this protein is more conserved (78%) among the different dengue serotypes, which elicits a strong cellular immune response after viral infection in humans and animal models. Present study, a central hydrophilic region of NS3 cofactor, NS2B (NS2BH) with full length of NS3 genes DENV3 Indonesian strain were amplified from CDNA following PCR, and inserted to PYES2/CT shuttle vector. The recombinant plasmid was transformed and expressed in Saccharomyces cerevisiae expression system. As result, detection with Anti-His detector and Anti-NS3 shown NS2BH/NS3 was expressed as 83 KDA protein band. We found that addition of NS2BH on NS3 full length construction plasmid increase the yield and activity of protein expression in S. cerevisiae. In future study, our recombinant NS2B/NS3 protein can be used as recombinant protein in Dengue vaccine development.
Co-Authors Abinawanto Abinawanto ASRI SULFIANTI DODDY IRAWAN SETYO UTOMO Ika Pratiwi Sabar Pambudi Wakhidah, Anisatu Z.