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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Tumbuhan Obat Indonesia Biotropika Research Journal of Life Science Natural B Berkala Penelitian Hayati
Retno Mastuti
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Genetic Variation Analysis of EMS-Induced Chili Pepper (Capsicum frutescens L.) Mutants Using SSR Markers Edia Fitri Dwinianti; Retno Mastuti; Estri Laras Arumingtyas
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mutation induction with chemical mutagen ethyl methane sulfonate (EMS) capable of producing genetic variation in plants. The purpose of this research was to evaluate genetic variation of EMS-induced chili pepper mutants using SSR molecular marker. Various concentration of EMS (0.01%; 0.02%; 0.04%) were applied to chili pepper seeds of Genotypes 2, 7, and 11. The genomic DNA was isolated from mutants and control plants then used for PCR analysis using three SSR primers namely CA26, CA52 and CA96. The three SSR primers were able to detect genetic variation in the three genotypes of EMS-induced chili pepper mutants and control plants. A total of 18 alleles have been identified in all three SSR loci with PIC values ranging from 0.792 to 0.893. The three genotypes of chili pepper plants had different sensitivity to EMS mutation induction. Genotype 11 plants showed higher sensitivity to EMS treatment than genotypes 2 and 7 based on the amount of genomic changes. Dendrogram constructed based on genetic similarity values distributed the mutants and control plants into three main groups with similarity coefficients ranging from 0.34 to 0.90. Similar genotype either control plant or mutants, especially Genotypes 2 and 7 were group into similar group. However, for the Genotype 11 plants, large genome changes cause mutant plants G11K1, G11K2, G11K3 had a low genetic similarity to their control plants, so the mutants were separated in a different group from the control plant. Those indicate that EMS mutation induction capable of increasing genetic variation in chili pepper plants based on SSR molecular marker.
The Dynamic of Calcium Oxalate (CaOx) in Porang Corms (Amorphophallus muelleri Blume) at Different Harvest Time Nurul Chairiyah; Nunung Harijati; Retno Mastuti
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.05

Abstract

The research aims to observe the influence of harvesting time on the change of calcium oxalate (CaOx) content and crystal density in Porang corms. The corms were harvested at different times, i.e., (1) two weeks before the plants shed (R0-1), (2) when the plants shed (R0), and (3) two weeks after the plants shed (R0+1). CaOx was obtained using the modified extracting method. Microscopic observations were obtained from the slices of the edge and center part of porang corms. Parameter observed including CaOx content, corm weight, shape, and density of CaOx crystal. CaOx content and crystal density in corms were analyzed using One way ANOVA. If the results are significant, it will be followed by Tukey Test α 0.05. In the meantime, the relation between CaOx content and corm weight was analyzed using Correlation Test Bivariate. The results showed that CaOx content was relatively higher in porang corms, i.e., 15.98 ± 0.60g/100g. On the other hand, the increasing of CaOx content might improve corm weight. The total density of druse, styloid, and prism crystal was pretty high in corms obtained when the plants shed compared to another harvest time, i.e., 1,494 ± 286; 31,280 ± 17,406 and 6,256 ± 1,533 crystals/cm2 . Raphide crystal density, by contrast, increased in corms obtained after the plants shed, i.e.,1,656 ± 368 crystals/cm2 . Total CaOx crystal density in the edge parts of corms harvested when the plants shed was proportionately higher than in the other harvest times, i.e., 12,292 ± 4,687.89 crystals/cm2 . In contrast, CaOx crystal densities in the center parts of corms were not much different at three harvesting times. The density of druse and prism crystals was somewhat higher in the center part of corms than in the edge parts. In opposition to, the density of raphide and styloid crystals was fairly higher in the edge part of corms than it was in the center parts. However, only raphide crystal density found in the edge and center part of corms was significantly affected by harvest time from all these results.
Co-Authors Aminatun Munawarti Bina Rizki Amalia Budi Waluyo Edia Fitri Dwinianti Estri Laras Arumingtyas Estri Laras Arumingtyas Hiroshi Miyake Imaniah Bazlina Wardani Jati Batoro Muhammad Naufal Afifi Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nunung Harijati Nurul Chairiyah Nurul Chairiyah Putri Rabiah Al Adawiah Rodiyati Azrianingsih Takeshi Taniguchi Varni Apensa Wahyu Widoretno Wahyu Widoretno Yoji Takeoka