<![CDATA[ABSTRACT Semarang city is one of leptospirosis endemic area in Central Java Province and the disease is always found annualy with its mortality rate had tendency to increase. The Case Fatality Rate (CFR) in the period 2014-2018 increased from 18% to 25%. Rodents, especially rats are the most important reservoir and a maintenance host. A survey was conducted in Semarang city from March to November 2016. Rats was trapped twice and their kidneys were tested for Leptospira by using PCR. Kidneys containing leptospira were then examined by sequencing. Trap success in Jangli was 15,41% consisting of R. norvegicus, R. tanezumi and insectivora S. murinus. The success trap in Gajahmungkur was 35,89% including Bandicota indica, Rattus norvegicus, Rattus tanezumi and insectivora Suncus murinus. Positive Leptospira was found in Jangli Village (38,09%) and Gajahmungkur Village (56,25%) The positive leptospira were from B. indica, R. norvegicus and R. tanezumi. Result of the sequencing revealed that nine from 17 sample homolog with pathogenic leptospira. Among rat populations, especially norway rats are specific serovar maintenance host in the region and are proved to carry a pathogenic Leptospira bacteria. ABSTRAK Kota Semarang merupakan salah satu daerah endemis leptospirosis dan penyakit tersebut selalu ditemukan setiap tahun dengan angka kematian yang cenderung meningkat. Pada periode 2014–2018 angka kematian leptospirosis meningkat dari 18% menjadi 25%. Tikus merupakan reservoir paling penting dan merupakan maintenance host Leptospira. Survei dilakukan di Kota Semarang yakni di daerah Kelurahan Jangli dan Kelurahan Gajahmungkur pada bulan maret-november 2016. Penangkapan tikus dilakukan sebanyak 2 kali dan diambil sampel ginjal untuk pemeriksaan Leptospira dengan Polymerase Chain Reaction (PCR). Ginjal dengan hasil PCR positif Leptospira dilanjutkan dengan tahapan sekuensing. Keberhasilan penangkapan tikus di Jangli 15,41% dengan spesies Rattus norvegicus, Rattus tanezumi dan Suncus murinus. Keberhasilan penangkapan tikus di Gajahmungkur 35,89% dengan spesies tikus Bandicota indica, Rattus norvegicus, Rattus tanezumi dan Suncus murinus. Positif Leptospira ditemukan di Kelurahan Jangli (38,09%) dan Kelurahan Gajahmungkur (56,25%). Hasil pemeriksaan PCR positif berasal dari spesies Bandicota indica, Rattus norvegicus dan Rattus tanezumi. Hasil sekuensing menunjukkan bahwa 9 dari 17 sampel mempunyai hubungan kekerabatan dengan Leptospira patogenik. Diantara populasi tikus, khususnya tikus got merupakan maintenance host serovar tertentu di wilayah tersebut dan terbukti membawa bakteri Leptospira patogenik.]]>
