A. Martina
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ISOLASI DAN SELEKSI JAMUR SELULOLITIK DARI HUTAN ARBORETUM UNIVERSITAS RIAU Hardianty, Denty Irma; Roza, R. M.; Martina, A.
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The Arboretum University of Riau constitutes of a secondary forest in Pekanbaru. Manyplant genera exist in this Arboretum, therefore the litter produced has high content ofcellulosa that may provide suitable environment for many cellulolitic fungus. Thepurpose of this study was to obtain the cellulolytic fungus isolates by screening the soilthat collected from Arboretum University Riau, using Potato Dextrose Agar medium.The selection of cellulolytic fungus used Carboxyl Methyl Cellulose medium and wasindicated by the appearance of clear zona. Cellulase activities were determinated usingthe ratio of clear zone and colony diameter. The result identified 53 cellulolytic fungusisolates. The L4H4 isolate had the highest ratio (3,51). There were three ratio criterionsof clear zone to colony diameter in this study, i.e. high (24,53%), medium (35,85%) andlow (39,62%). The characterization result showed that 15 fungus isolates belonged tothe genera Aspergillus, Penicillium, Paecilomyces, Mucor and 2 isolates wereunidentified.
POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Ralstonia solanacearum Dwiana Inggriani; Rodesia M. Roza; A. Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Ralstonia solanacearum is one of soil pathogen bacteria that attack horticulture plant.Chemical pesticides are used commonly to control bacteria. Using chemical pesticidescontinously can give negative effect for living things and the evironment. Some soilmicrobes produce antibacterial compounds. This study aimed to test the potential ofpeat land microbes at Rimbo Panjang Kampar to inhibit Ralstonia solanacearum. Thisstudy used agar disk method to calculate the ratio activity between the inhibition zoneand the colony diameter (Z/K) and then grouped into high, medium and low criteria.Twenty isolates of fungi that produced antibacteria against Ralstonia solanacearumconsisted of genus Penicillium and Trichoderma. Isolate RPL2-29 (Penicillium) had thehighest ratio (2.12) and isolate RPL2-38 (Penicillium) had the lowest ratio (1.14).Eleven isolates of actinomycetes that produced antibacteria consisted of Streptomyces,Micromonospora while 2 isolates has not been unidentified.Isolate RB3S51(Streptomyces sp.) had the highest ratio (2.87) and RB3S57 (Streptomyces sp.) had thelowest ratio (1.29).The high criteria was dominated by actinomycetes isolates of thegenus Streptomyces. Isolate RB3S51 (Streptomyces sp.) had the highest ratio (2.87) andthe lowest ratio of fungal isolates RPL2-38 with ratio 1.14. Based on thecharacterization, the fungi were classified into the genus Penicillium.