Rodesia M. Roza
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POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Ralstonia solanacearum Dwiana Inggriani; Rodesia M. Roza; A. Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Ralstonia solanacearum is one of soil pathogen bacteria that attack horticulture plant.Chemical pesticides are used commonly to control bacteria. Using chemical pesticidescontinously can give negative effect for living things and the evironment. Some soilmicrobes produce antibacterial compounds. This study aimed to test the potential ofpeat land microbes at Rimbo Panjang Kampar to inhibit Ralstonia solanacearum. Thisstudy used agar disk method to calculate the ratio activity between the inhibition zoneand the colony diameter (Z/K) and then grouped into high, medium and low criteria.Twenty isolates of fungi that produced antibacteria against Ralstonia solanacearumconsisted of genus Penicillium and Trichoderma. Isolate RPL2-29 (Penicillium) had thehighest ratio (2.12) and isolate RPL2-38 (Penicillium) had the lowest ratio (1.14).Eleven isolates of actinomycetes that produced antibacteria consisted of Streptomyces,Micromonospora while 2 isolates has not been unidentified.Isolate RB3S51(Streptomyces sp.) had the highest ratio (2.87) and RB3S57 (Streptomyces sp.) had thelowest ratio (1.29).The high criteria was dominated by actinomycetes isolates of thegenus Streptomyces. Isolate RB3S51 (Streptomyces sp.) had the highest ratio (2.87) andthe lowest ratio of fungal isolates RPL2-38 with ratio 1.14. Based on thecharacterization, the fungi were classified into the genus Penicillium.
ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI FENOL DARI LIMBAH CAIR RUMAH SAKIT UMUM DAERAH (RSUD) ARIFIN ACHMAD PEKANBARU Rahmy Juwita; Bernadeta L. Fibriarti; Rodesia M. Roza
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Phenol is a toxic organic compound found in hospital liquid waste. This research was conducted to obtain bacterial isolates that can be used for degrading agent in the microbiologically liquid waste control without harmful side product. Samples weretaken from the liquid waste tank, at Arifin Achmad Hospital Pekanbaru, then the phenol countent was counted using a folin-ciocalteau method. The isolation of phenol degrading bacteria used enrichment culture method in microbiology laboratory of Faculty of Mathemathic and Science, Riau University. The next steps were to purify isolates purification, to test the ability of phenol degradation and also to obtainmorphological characteristic (simple and gram staining) and to have some biochemical tests (MR-VP test, catalase test, carbohydrates fermentation, simmon’s citrate test, casein hydrolysis and indole test). The results showed that phenol content in the samples ranged from 2 to 6 ppm, then 6 bacterial isolates were randomly selected and grown at ramsay medium and enriched with phenol in 48 hour incubation time. The shapes of selected isolates were bacilli and gram negative. The highest degradation rate was on MJEQ1 isolates (10 ppm/hour) and the lowest degradation was on MJST2isolates (7,2 ppm/hour).
ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT KEMASAN YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Ike Yuliana; Rodesia M. Roza; Atria Martina
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Yoghurt is a fermented product of a group of lactic acid bacteria (LAB) on milk that hasbeen pasteurized. LAB is a group of bacteria that are able to convert carbohydrates(glucose) into lactic acid. LAB produces metabolites that inhibit the growth ofpathogenic bacteria. This study aimed to isolate LAB from yoghurt packaging with itsinhibitory against bacterial pathogens Escherichia coli and Salmonella typhi. LAB wasisolated from 9 samples with 3 brand products and 3 different flavors. Calculation of thetotal population to the overall LAB samples was obtained between 6.4 × 10 6 cfu/ml to2.1 × 10 7 cfu/ml. In this study, inhibition test of pathogenic bacteria E. coli and S. typhiused samples yoghurt. The method used is the well-agar diffusion. The most effectiveyoghurt in inhibiting E. coli was yogurt Ab with diameter of inhibiting zone 23.55 mm,while the yogurt sample that had the highest inhibitory zone which is 19.93 mm forS.typhi was yoghurt Ap.