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All Journal REINWARDTIA Manilkara: Journal of Bioscience Reinwardtia
I PUTU GEDE P. DAMAYANTO
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Journal : Manilkara: Journal of Bioscience

 1 
Keanekaragaman Spesies Bambusa (Poaceae, Bambusoideae) di Kalimantan, Indonesia Anne Kusumawaty; I Putu Gede P. Damayanto
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7516.2024

Abstract

Bamboo is reported to have many benefits, including as handicraft material, fishing, building materials, household appliances, furniture, food containers, medicines, vegetable materials, and firewood. In Indonesia, bamboo species richness is quite high with 175 bamboo species that can be found spread from Java to Papua. Research information on Bambusa species data in the Kalimantan region is relatively scarce and invalid. The research aims to provide data on the diversity of Bambusa species in Kalimantan. The research method was carried out descriptively by observing morphological characters from herbarium specimens and digital herbarium. Data were collected from herbarium specimens of bamboo genus Bambusa collected from Kalimantan and digital specimen data stored in online database portals, such as GBIF and Bioportal. All specimens were re-identified and their names validated. The results showed that there are five species of Bambusa in Kalimantan, namely Bambusa glaucophylla Widjaja, B. multiplex (Lour.) Raeusch. ex Schult.f., B. spinosa Roxb., B. tuldoides Munro, and B. vulgaris Schrad.  The presentation is complemented by identification keys and explanations.
Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia) Susila, Susila; I Putu Gede P. Damayanto; Kusumadewi Sri Yulita; Nawwall Arrofaha
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7517.2024

Abstract

Rosewood (Dalbergia latifolia) is a hardwood plant, resistant to termite attack, its wood has beautiful patterned fibers and textures, so it is widely used for furniture in luxury categories with high economic value. According to CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), rosewood trade status is included in appendix II. In the trade of rosewood in both domestic and international markets, there are relatively many obstacles and difficulties in identifying the origin of rosewood. It is very important to establish a reference on the identity of wood based on the location of origin as an effort to prevent illegal trade, namely by using a molecular-based identification approach (DNA fingerprinting). In the isolation of rosewood DNA, special techniques are required due to its hard wood characteristics. This study aims to compare DNA isolation techniques from wood and leaves of rosewood using the CTAB (Cetyl Trimethyl Ammonium Bromide) protocol and the commercial kit protocol. The research used molecular biology-based identification methods. PCR results showed that the DNA isolation protocol using the commercial kit outperformed the CTAB protocol for both leaf and wood samples. DNA isolation from wood samples was more difficult than leaf samples. Overall, the best protocol for DNA isolation from rosewood leaves and wood was to use a commercial kit.
Co-Authors alex sumadijaya Anne Kusumawaty Ary PRIHARDHYANTO Keim Daniel Happy Putra Elizabeth A. widjaja HIMMAH RUSTIAMI Ina Erlinawati Joko Ridho Witono Kusumadewi Sri Yulita Lulut D. Sulistyaningsih Marlina Ardiyani Miftahudin . Nawwall Arrofaha Ni Putu Sri Asih Rayhanissa Susila, Susila TATIK CHIKMAWATI Yuzammi