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Urnemi Urnemi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering

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APLIKASI MARKA MOLEKULER PADA BUAH DAN BIJI KOPI ASAL KALIMANTAN TIMUR Fatimah Fatimah; Urnemi Urnemi; Apon Zaenal Mustopa; Hudaida Syahrumsyah
BERITA BIOLOGI Vol 13, No 1 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i1.649

Abstract

Two quantitative traits, cherry and green bean characters are the important phenotypic selection in coffee breeding practice. The important well-known character from coffee markets is cherry and bean size. In this study, 43 genotypes of coffee were collected from four districts in East Kalimantan i.e. Kutai Kertanegara, Kutai Timur, Berau dan Paser Utara. The objective of this study was to identify cherry and green bean character using quantitative trait locus (QTL) molecular marker, genetic variation from developed alleles, cluster analysis and association analysis of molecular marker, and phenotype observation. Based on polymorphic information content (PIC) of primers used in this study, the genetic variation was low. Based on cluster analysis, two major groups were identified. The first group corresponds to Arabika that consisted of 3 districts, Kutai Timur, Berau and Paser Utara. The second group correspond to Robusta mostly from Kutai Kertanegara.Significant association of primer markers M480 and M312 with QTL has suggested that they can be used as specific primers linked to size of cherry and green bean.Furthermore,they were potential marker assisted breeding in coffee breeding program.
Potensi Bakteri Asam Laktat Sebagai Kandidat Probiotik Antimikroba Patogen Asal Fermentasi Kakao Varietas Criollo Urnemi Urnemi; Sumaryati Syukur; Endang Purwati; Sanusi Ibrahim; Jamsari Jamsari
Jurnal Riset Teknologi Industri Vol 6 No 12 Desember 2012
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8019.535 KB) | DOI: 10.26578/jrti.v6i12.1519

Abstract

Lactic Acid Bacteria (LAB) were isolated from cocoa beans three days fermentation Criollo variety. That were eleven isolates, all of isolates were named Gr3 (1, 2, 3, 4,…,11). All of isolates were tested to antimicrobial activity against indicator pathogenic bacteria (Escherichia coli  14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, Listeria monocytogenesis dan Salmonella typhii),  than that was chosen potential one isolate had highest inhibition zones to pathogens.  One potential isolate was done determination weigh protein bacteriocin by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Result of antimicrobial activity test showed that, 11 isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 11 mm at 48 hours observation. That was found one isolate potential, it was Gr3.7.  Gr3.7 isolate had high inhibition zones to against growth pathogenic bacteria, that was 11mm to Salmonella typhii and Listeria monocytogenesis at 48 hours observation. Determination crude weight protein test was estimated 24 kDa protein by using SDS-PAGE.  To Found purifying weight protein bacteriocin must be done purification processing. Probiotic candidate (Gr3.7) can be used as natural antibiotics, because it had positive responce to against Salmonella typhii.  Salmonella typhii was known pathogen in intestine,  beside that it was used as food preservative, because it had positive response to against Listeria monocytogenesis.   
Co-Authors APON ZAENAL MUSTOPA Fatimah Fatimah Hudaida Syahrumsyah Jamsari Jamsari Sanusi Ibrahim Sumaryati Syukur ZK Abdurahman Baizal