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Gleni Hasan Huwoyon
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Engineering Environmental Science

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ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS Rudhy Gustiano; Tia Oktaviani; Dinar Tri Soelistyowati; Irin Iriana Kusmini; Wahyutomo Wahyutomo; Gleni Hasan Huwoyon
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.641

Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.
PERBANDINGAN PERTUMBUHAN IKAN NILA BEST F6, NILA BEST F5 DAN NILA NIRWANA PADA PENDEDERAN I-III DI JARING APUNG DANAU LIDO Irin Iriana Kusmini; Rudhy Gustiano; Gleni Hasan Huwoyon; Fera Permata Putri
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 22 No. 2 (2015)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2015.13308

Abstract

Penelitian ini bertujuan untuk mendapatkan perbandingan pertumbuhanantara ikan Nila BEST generasi ke enam (F6), ikan Nila BEST generasi ke lima (F5), ikan Nila F6 kontrol dan ikan Nila Nirwana pada pendederan I-III. Kegiatan dilaksanakan di Danau Lido, Jawa Barat, terdiri dari tiga ulangan dengan menggunakan jaring berukuran 2x2x1 m3sebanyak 12 unit, padat tebar 100 ekor/m2. Pemberian pakan tiga kali sehari sebesar 5% dari biomassa, kadar protein 25%. Pengambilan sampel secara acak dilakukan sekali dalam satu bulan. Dari masing-masing populasi diambil sebanyak 30 ekor untuk diukur panjang dan bobotnya. Hasil penelitian menunjukkan bahwa pertumbuhan bobot BEST F6 unggul 22,28% dibandingkan ikan Nila Nirwana, 5,64 % dibandingkan BEST F5 dan 18,51% dibandingkan ikan Nila F6 kontrol. Pertumbuhan panjang BEST F6 unggul 1,92% dibandingkan BEST F5, 7,9% dibandingkan Nila Nirwana dan 5,52% dibandingkanF6 kontrol dan rasio konsumsi pakan (FCR) terkecil terdapat pada BEST F6. Nilai ini menunjukkan bahwa seleksi dapat meningkatkan pertumbuhan karena diduga ada perbaikan gen yang mengontrol pertumbuhan ikan, namun BEST F6 memiliki SR yang rendah dibandingkan dengan ikan Nila uji lainnya. Hal ini merupakan petunjuk bahwa program seleksi tidak menjamin kesempurnaan individu baru yang dihasilkan seperti halnya ketahanan terhadap kondisi oksigen terlarut yang rendah.
Co-Authors Dinar Tri Soelistyowati Fera Permata Putri Irin Iriana Kusmini Rudhy Gustiano Rudhy Gustiano Tia Oktaviani Wahyutomo Wahyutomo