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RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.892 KB) | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)] Gustiano, Rudhy; Oktaviani, Tia; Soelistyowati, Dinar Tri; Kusmini, Irin Iriana; Wahyutomo, Wahyutomo; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.515 KB) | DOI: 10.14203/bb.v12i3.641

Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.
KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Mahardhika, Prana; Gustiano, Rudhy; Soelistyowati, Dinar Tri; Ath-thar, MH Fariduddin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.291 KB) | DOI: 10.14203/beritabiologi.v10i4.771

Abstract

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 ± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 ±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.
QUALITATIVE AND QUANTITATIVE CHARACTERS OF THREE COMMON CARP (Cyprinus carpio L.) STRAINS IN INDONESIA Harton Arfah; Dinartri Soelistyowati
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 11 No. 2 (2004): Desember 2004
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.652 KB)

Abstract

The second generation of gynogenetic diploid common carp of majalaya, sinyonya and mirror strains have been produced in 1992. Some of them were sex-reversed by oral administration of 17 α-methyltestoteronne. The females and sex-reversed males were matured on early 1994 and mated to produce progenies ofpure strains. This research aimed to measure certain quantitative characters since the early stage of developmentto the market size. Three different conditions of rearing (i.e. rice field, ponds and concrete tanks) were applied during the nursery phase, while the earthen pond and running water system with intensive feeding were used for the grow-out stage. The results showed that average fecundity of sinyonya carp was higherthan mirror and majalaya strains, but its performances of the early development (i.e. fertilization rate, hatching  rate and survival rate at 14 days old) was lowest. At the nursery phase, the survival and growth rates of majalaya carp was relatively better than the other two strains, but the mirror carp had highest feed efficiency and lowest protein retention. During culture period, the growth rates of these three common carp strains were relatively similar (1%), but sinyonya carp, among strains had the highest percentage of fillet (38.5%) with the  lowest total number of muscular bones (69), the highest level of protein content (77.5%) and the lowest level  of lipid content (16.6%) among the three strains. Keywords: Cyprinus carpio L, gynogenesis, growth rate, product quality.
Synodontis eupterus Larvae Masculinization Using Javanese Long Pepper Extract (Piper retrofractum) Prassetyo Dwi Dhany Wijaya; Muhammad Zairin Junior; Dinar Tri Soelistyowati; Widanarni Widanarni
Journal Omni-Akuatika Vol 13, No 1 (2017): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.451 KB) | DOI: 10.20884/1.oa.2017.13.1.224

Abstract

Synodontis eupterus male represents only 5-10 % of farmer total synodontis production, which limits male availability for reproduction purpose. A method widely used in overcoming the low male production was masculinization through synthetic hormones, which was restricted due to its adverse impact on the environment. Natural ingredients from plant were demonstrated in various studies to substitute the 17α-methyltestosteron hormone on masculinization. This research aimed at evaluating the effects of Javanese long pepper extract (JLP) through immersion on synodontis fish larvae. The research was conducted using factorial design with two faktor (4x2) consisted of eight treatments: 17α-methyltestosteron 2 mg L-1 (MT) immersion for 5 and 10 hours, JLP dose 0.125 mg L-1 (P1) immersion for 5 and 10 hours and dose 0.25 mg L-1 (P2) immersion for 5 and 10 hours compared to the control treatment without immersion (P0). A hundred of synodontis larvae of 10 days old after hatching each replication were used in the immersion treatments. The results showed that JLP treatments produced 25-40 % of male synodontis age four months, 1-2 % females and above 60 % intersex. While at age five months, the percentage of intersex fish decreased to 20-40 %, the female fish increased to  60-62 %, and male fish ranged 20-35 % in JLP compared to the control P0 (15 %). The dose of JLP 0.25 mg L-1 increased mortality of about 14-54 %.
KELANGSUNGAN HIDUP LOBSTER PASIR Panulirus homarus YANG DIPELIHARA PADA SISTEM RESIRKULASI Donny Prariska; Eddy Supriyono; Dinar Tri Soelistyowati; Rizki Eka Puteri; Selly Ratna Sari; Raudhatus Sa'adah; Guttifera
Clarias : Jurnal Perikanan Air Tawar Vol. 1 No. 1 (2020): Jurnal Perikanan Air Tawar "Clarias"
Publisher : Universitas Sumatera Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.726 KB) | DOI: 10.56869/clarias.v1i1.52

Abstract

Penelitian ini bertujuan untuk mengevaluasi padat tebar terhadap tingkat kelangsungan hidup dan respon fisiologis terbaik pada pemeliharaan lobster pasir yang dipelihara pada sistem resirkulasi. Penelitian dilaksanakan dengan menggunakan rancangan acak lengkap (RAL) dengan 3 perlakuan kepadatan lobster pasir berbeda, yaitu kepadatan 15 ekor/m2, 25 ekor/m2 dan 35 ekor/m2 dengan 2 kali ulangan. Penelitian dilakukan dengan menggunakan lobster berukuran bobot rata-rata 83.13±1.11 g yang dipelihara selama 30 hari. Parameter yang diamati dalam penelitian ini meliputi kualitas air, respon fisiologi seperti frekuensi molting dan kinerja produksi. Hasil penelitian menunjukkan padat penebaran lobster Panurilus homarus terbaik adalah kepaadatan 35 ekor/m2 dengan kelangsungan hidup mencapai 95,71%, frekunsi molting mencapai 14,3% dan koversi pakan selama penelitian adalah 7,1. Bobot dan panjang akhir yang dari setiap perlakuan adalah 113,72±7,34 gram dan 128,21±1,76 cm. Padat penebaran tertinggi mampu meningkatkan efisiensi dan efektivitas dalam produksi budidaya, meskipun pada parameter pertumbuhan, tingkat kelangsungan hidup dan respon fisiologi tidak menunjukkan hasil berbeda nyata pada tiap perlakuan.
ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS Rudhy Gustiano; Tia Oktaviani; Dinar Tri Soelistyowati; Irin Iriana Kusmini; Wahyutomo Wahyutomo; Gleni Hasan Huwoyon
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.641

Abstract

In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.
KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Prana Mahardhika; Rudhy Gustiano; Dinar Tri Soelistyowati; MH Fariduddin Ath-thar
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.771

Abstract

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 ± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 ±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS Iskandariah Iskandariah; Dinar Tri Soelistyowati; Rudhy Gustiano; Irin Iriana Kusmini; Gleni Hasan Huwoyon
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
Embriogenesis, Perkembangan Larva dan Viabilitas Reproduksi Ikan Pelangi Iriatherina Werneri Meinken, 1974 pada Kondisi Laboratorium Muh. Herjayanto; Odang Carman; Dinar Tri Soelistyowati
Akuatika Indonesia Vol 2, No 1 (2017): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.43 KB) | DOI: 10.24198/jaki.v2i1.23389

Abstract

Ikan pelangi Iriatherina werneri diperdagangkan sebagai ikan hias, karena memiliki warna dan bentuk sirip yang indah terutama untuk individu jantan. Perkembangan budidaya ikan pelangi terkendala oleh kurangnya informasi biologi terkait perkembangan embrio (embriogenesis), perkembangan larva, viabilitas reproduksi dan sejarah kehidupannya. Tujuan dari penelitian untuk mengkaji embriogenesis, perkembangan larva dan viabilitas reproduksi pada ikan pelangi I. werneri sebagai informasi dasar untuk menunjang kegiatan budidaya dan konservasi. Pengamatan embriogenesis menggunakan 100 butir embrio yang dimasukkan pada empat buah wadah inkubasi berukuran (19,5 × 13,5 × 8,5) cm3. Pengamatan viabilitas reproduksi dan pertumbuhan menggunakan 200 butir embrio yang dimasukkan pada lima buah wadah berukuran (19,5 × 19,5 × 19,5) cm3. Hasil penelitian menunjukkan bahwa embrio ikan pelangi berkembang mulai dari fase pembelahan sel, morula, blastula, gastrula, organogenesis dan menetas menjadi larva pada saat 124 jam 5 menit setelah pembuahan. Telur mulai menetas pada hari keempat (15,11%) dan berakhir pada hari keenam (23,35%) dengan puncak penetasan terjadi pada hari kelima (61,54%) setelah pembuahan. Ikan telah menjadi juvenil pada umur 35 hari setelah menetas dengan panjang total >12 mm. Viabilitas reproduksi I. werneri pada penelitian ini yaitu tingkat penetasan sebesar 62,04%; tingkat kelangsungan hidup sampai umur 50 hari setelah menetas sebesar 62,72% dan ikan jantan secara alami sebanyak 20%.
Co-Authors , Alimuddin , Rahman, , Achmad Sudradjat Agus Oman Sudrajat Ahmad Fahrul Syarif Ahmad Muzaki Ahmad Teduh Akbar, Muhamad Saepul Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Anang Hari Kristanto Anang Hari Kristanto Aras, Annisa Khairani Arifah Ambarwati Asep Bulkini Bagus Rahmat Basuki Daniel Djokosetiyanto Dendi Hidayatullah, Dendi Deni Radona Deni Radona Dian Hardiantho Dian Hardianto Didik Ariyanto Didik Ariyanto Donny Prariska Eddy Supriyono Edison, Thomas Erlania Erlania Euis Rakhmawati Fajar Maulana . Farah Diana Fauzan, Agung Luthfi Firmansyah, Rodhi Fitriyah Husnul Khotimah Fitriyah Husnul Khotimah Flandrianto S. Palimirmo Gleni Hasan Huwoyon Gleni Hasan Huwoyon Gleni Hasan Huwoyon, Gleni Hasan Gloria I. Satriani Gloria Ika Satriani Gloria Ika Satriani Gunawan, Bambang Kusmayadi Gusti Ngurah Permana Guttifera Hafidah, Riva Hafif Syahputra Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Helena Sahusilawane Herjayanto, Muh. Hidayatush Sholihin Ibrahim Satrio Faqih Ibrahim, Yusran Ida Ayu Amarilia Dewi Murni Imron Imron, Imron Indah Mastuti Intan Putriana Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini Irin Iriana Kusmini, Irin Iriana Irwan Irwan Irzal Effendi Iskandariah Iskandariah Iskandariah, Iskandariah Kesit Tisna Wibowo Ketut Mahardika Ketut Sugama Ketut Sugama Komar Sumantadinata Kukuh Nirmala Ligaya I. T. A. Tumbelaka M. H. Fariduddin Ath-thar M. Syukur M. Zairin Junior Mahardhika, Prana Mahdaliana, Mahdaliana Melta Rini Fahmi MH. Fariduddin Ath-thar Mia Setiawati Muh. Herjayanto Muhamad Syukur Muhammad Fadlan Furqon Muhammad Hunaina Fariduddin Ath-thar Muhammad Sami Daryanto Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Muliari Muliari Mulyasari Mulyasari Nopri Yanto Odang Carman Oktaviani, Tia Peni Pitriani Poppy Dea Bertha, Poppy Dea Prana Mahardhika Prassetyo Dwi Dhany Wijaya Rahmadani Rahmadani Ratu Siti Aliah Raudhatus Sa'adah Rezki, Dinda Wahyu RIDWAN AFFANDI Rinaldi Rinaldi Rini Susilowati Riva Hafidah Rizki Eka Puteri Ronny Rachman Noor Ruby Vidia Kusumah Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Safira Qisthina Ayuningtyas, Safira Qisthina Sahusilawane, Helena Afia Sandra, Aan Aryanti Sari Budi Moria Sembiring Sari Budi Moria Sembiring Selly Ratna Sari Sri Nuryati Sri Sundari Sudarto Sudarto Syahril, Alfis Tia Oktaviani Upmal Deswira Vitas Atmadi Prakoso Wahyutomo Wahyutomo Wahyutomo, Wahyutomo Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wildan Nurussalam Wiyoto Wiyoto Yeni Elisdiana Yogi Himawan