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ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG Prilya Dewi Fitriasari; Nanda Amalia; Susiyanti Farkhiyah
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3828

Abstract

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.