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All Journal JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Ilmiah Berkala: Sains dan Terapan Kimia
Muhammad Yuwono
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Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Medicine & Pharmacology Other

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Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin Isnaeni Isnaeni; Andri Astuti; Muhammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 4 No. 1 (2017): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.375 KB) | DOI: 10.20473/jfiki.v4i12017.34-38

Abstract

Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24 hours. The method was validated corresponding to linearity, limit of detection (LOD), intra day precision, and accuracy parameters. The accuracy was measured using streptomycin injection as a sample. Results: The Results showed that the KH2PO4 solution at 7.5% concentration was found to be the optimized solvent with Rf value of 0.5. The linear equation was y = 10.176x + 4.046 at 150 - 350 µg/mL concentration range with the linearity coefficient, Limit of Detection, accuracy, and variation coefficient were 0.9907; 40 ppm; 96.37 + 2.22% (with an RSD value of 2.31%); and 1.63 respectively. Conclusion: The prospective TLC-bioautographic method was applied for the identification and determination of streptomycin in a preparation using a single eluent KH2PO4. The eluent system optimization remains necessary for the identification and determination of the mixture of streptomycin with other antibiotics, such as aminoglycoside groups.
KAJIAN FARMAKOGNOSTIK SIMPLISIA DAUN KARAMUNTING (Rhodomyrtus tomentosa) ASAL PELAIHARI KALIMANTAN SELATAN sutomo sutomo; Arnida Arnida; Febri Hernawati; Muhammad Yuwono
Jurnal Sains dan Terapan Kimia Vol 4, No 1 (2010)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1141.383 KB) | DOI: 10.20527/jstk.v4i1.2048

Abstract

Karamunting telah lama digunakan oleh masyarakat sebagai obat tradisional. Dilihat dari prospek yang sangat potensial sebagai bahan obat maka perlu dilakukan kajian farmakognostik sampel untuk pengendalian mutu dan keaslian simplisia. Penelitian ini bertujuan memberikan dasar ilmiah mengenai gambaran farmakognostik secara kualitatif dan kuantitatif. Hasil penelitian secara kualitatif dan kuantitatif telah dideskripsikan. Identifikasi kimia menunjukkan hasil positif terhadap aleuron, tanin, katekol, alkaloid dan saponin. Karakteristik farmakognostik secara kuantitatif yaitu kadar abu sebesar 3,1%, kadar abu tidak larut asam 2,89%, kadar abu larut air 1,69%, susut pengeringan 14%, kadar sari larut air 0,48%, kadar sari larut etanol sebesar 0,36% dan bahan organik asing 0%. Hasil kromatogram diperoleh senyawa spesifik pada fase gerak kloroform : metanol : butanol (15: 2: 1) dengan nilai Rf 0,72 pada pengamatan dibawah lampu UV254 dan UV366. Pada fase gerak n-heksana : etil asetat (8 : 2) diperoleh dua senyawa yang spesifik dengan nilai Rf masing-masing 0,65 dan 0,78 dengan pengamatan dibawah lampu UV254 dan UV366. Kata kunci : Rhodomyrtus tomentosa, farmakognostik, identifikasi
Co-Authors Andri Astuti Arnida Arnida Febri Hernawati Isnaeni Sutomo Sutomo