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Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient Sisca Meida Wati; Istiati Istiati; Pratiwi Soesilawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 3 (2014): September 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Human periodontal diseases are inflammatory disorders as the result of complex interactions between periodontopathogens and the host’s immune response. Periodontitis results in tooth loss and can even lead to systemic diseases if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamated sites. Polymorphonuclear leukocyte (PMNs) infiltration can be seen in each stage of periodontitis. Lactoferrin is one of the PMN specific granules and could be a useful marker of PMN activity. Purpose: The aim of this study was to determine the band intensity of lactoferrin used as periodontitis biomarker. Methods: Gingival crevicular fluid (GCF) samples were collected using paper point no.30 from 40 subjects, 30 periodontitis patients that devide according to the severity (10 mild periodontitis, 10 moderate periodontitis, and 10 severe periodontitis) and 10 healthy controls, ranging in ages from 20 to 35 years. GCF lactoferrin was analyzed by Western blot and measured the band intensity by quantity one software (bio-rad). Results: The periodontitis sites exhibited significantly greater band intensity of lactoferrin than healthy sites. The band intensity of lactoferrin was positively correlated with the severity of periodontitis (α = 0.05). Conclusion: The study showed that the intensity of the lactoferrin protein bands could be used as biomarkers of periodontitis.Latar belakang: Penyakit periodontal adalah gangguan inflamasi yang merupakan hasil dari interaksi yang kompleks antara periodontopathogens dan respon imun host. Periodontitis mengakibatkan hilangnya gigi dan bahkan dapat menyebabkan penyakit sistemik jika tidak diobati. Cairan sulkus gingiva (GCF) mencerminkan kondisi gingiva dan mengandung protein yang tertransudasi dari serum atau sel pada lokasi radang. Infiltrasi polymorphonuclear leukosit (PMN) dapat dilihat pada setiap tahap periodontitis. Laktoferin adalah salah satu granula spesifik PMN dan bisa menjadi indicator aktivitas PMN. Tujuan: Tujuan penelitian ini adalah untuk meneliti intensitas band laktoferin dapat sebagai biomarker periodontitis. Metode: Cairan sulkus gingiva (GCF) dari tiap sampel dikumpulkan menggunakan paper pint no. 30 dari 40 subjek, 30 pasien dengan periodontitis yang dibagi sesuai dengan tingkat keparahan (10 periodontitis ringan, 10 periodontitis moderat, dan 10 periodontitis parah) dan 10 kontrol, mulai usia 20-35 tahun. GCF lactoferrin dianalisis dengan Western blot dan diukur intensitas bandnya dengan quantity one software (bio-rad). Hasil: Pada jaringan yang mengalami periodontitis menunjukkan intensitas band yang secara signifikan lebih besar dari laktoferin daripada periodontal yang sehat. Intensitas band laktoferin berkorelasi positif dengan tingkat keparahan periodontitis (α = 0,05) Simpulan: Hasil penelitian ini menyimpulkan bahwa intensitas band protein laktoferin dapat digunakan sebagai biomarker periodontitis.

Effect of Robusta coffee beans ointment on full thickness wound healing Yorinta Putri Kenisa; Istiati Istiati; Wisnu Setyari J
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traumatic lesions, whether chemical, physical, or thermal in nature, are among the most common lesion in the mouth. Wound healing is essential for the maintenance of normal structure, function, and survival of organisms. Experiments of Robusta coffee powder on rat-induced alloxan incision wound, clinically demonstrated similar healing rate with the povidone iodine 10%. No studies that look directly the effect of coffee extract in ointment form when viewed in terms of histopathology. Robusta coffee bean (Coffea canephora) consists of chlorogenic acid (CGA) and caffeic acid which are belived to act as antioxidant and take part in wound healing process. Purpose: The aim of this study was to identify the enhancement of healing process of full-thickness skin wound after Robusta coffee beans extract ointment application. Methods: Sample consisted of 20 Cavia cabaya treated with full-thickness with wounds and was given Robusta coffee beans extract ointment concentration range of 22.5%, 45%, and 90% except the control group which was given ointment base material. Animals were then harvested on the fourth day and made for histopathological preparations. Data were calculated and compared by one-way ANOVA test and LSD test. Results: The study showed that Robusta coffee bean extract ointment can increase the number of lymphocytes, plasma cells, macrophages, fibroblasts, and blood vessels by the presence of chlorogenic acid (CGA) and Caffeic acid. Conclusion: In conclusion Robusta coffee bean extract ointment enhance the healing process of fullthickness skin wound of Cavia cabaya.Latar belakang: Lesi traumatik, baik akibat rangsang kimia, fisik, atau termal, merupakan lesi yang paling umum terjadi di dalam rongga mulut. Penyembuhan luka yang terjadi ini penting untuk pemeliharaan struktur normal, fungsi, dan kelangsungan hidup organisme. Percobaan pemberian bubuk kopi Robusta terhadap luka sayatan pada tikus yang diinduksi aloksan, secara klinis menunjukkan tingkat penyembuhan yang sama dengan povidone iodine 10%. Namun belum ada penelitian yang melihat secara langsung pengaruh ekstrak kopi dalam bentuk salep bila dilihat dari segi histopatologi. Biji kopi Robusta (Coffea canephora) terdiri dari chlorogenic acid (CGA) dan caffeic acid yang dipercaya berperan sebagai antioksidan dan mengambil bagian dalam proses penyembuhan luka. Tujuan: Tujuan penelitian ini adalah untuk mengidentifikasi peningkatan proses penyembuhan luka full-thickness pada kulit setelah pengaplikasian salep ekstrak biji kopi Robusta. Metode: Sampel terdiri dari 20 Cavia cabaya yang diberi perlakuan berupa luka sayat full-thickness pada kulit punggung dan diberi salep ekstrak biji kopi Robusta dengan beberapa konsentrasi, yaitu 22,5%, 45%, dan 90%, sedangkan kelompok kontrol hanya diberi bahan dasar salep. Binatang coba kemudian dieksekusi pada hari keempat dan dibuat sediaan histopatologinya. Data dihitung dan dibandingkan dengan uji One-Way ANOVA dan uji LSD. Hasil: Hasil penelitian menunjukkan bahwa salep ekstrak biji kopi Robusta dapat meningkatkan jumlah limfosit, sel plasma, makrofag, fibroblas, dan pembuluh darah yang dipengaruhi oleh chlorogenic acid (CGA) dan caffeic acid. Kesimpulan: Disimpulkan bahwa salep ekstrak biji kopi Robusta memiliki efek dapat meningkatkan proses penyembuhan luka full-thickness pada kulit Cavia cabaya.

The role of ubiquinone supplementation on osteogenesis of nonvascularized autogenous bone graft Irham Taufiqurrahman; Achmad Harijadi; Roberto M. Simanjuntak; Coen Pramono D; Istiati Istiati
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Ubiquinone is one of food supplement which is known have positive effect in wound healing. However the study to evaluate the possible role of ubiquinone in bone healing in autogenous bone grafting after mandibular resection has not been studied. An in vitro study is required to evaluate whether ubiquinone or coenzyme Q-10 (CoQ10) has a positive effect on osteogenesis. Viability test of CoQ10 and a model of osteogenic-induced and hypoxic-condition mesenchymal stem cell culture were established to support the study. Purpose: The study was made to evaluate the role of ubiquinone in osteogenesis by analyzing the toxicity effect and the optimal dose of CoQ10 that might interfere in bone marrow derived mesenchymal stem cell (BM-MSC) that was dose in cell culture medium. The BM-MSC culture under hypoxia condition were also observed. Method: The toxicity and the optimum viability concentration of ubiquinone were observed using MTT assay. The osteogenic differentiation under hypoxic condition was done on BM-MSC in osteogenic medium that composed of ascorbic acid, glycerolphosphate and dexamethasone in hypoxia chamber for 21 days. Osteogenic differentiation and cellular hypoxia features were tested with immunocytochemical staining using anti-Runx2 and anti-HIF1α monoclonal antibody, respectively. Result: The maximum density value of 1.826 was found in the group of ubiquinone concentration of 75μM/ml, increasing of in concentration of ubiquinone resulted with the decrease ofoptical density of CoQ10. Statistic analysis using Anova showed with no significant difference among groups with various concentration. Immunocytochemical staining showed that Runx2 expression in 3% hypoxia group (p<0.05). Conclusion:Ubiquinone was found non toxic in its optimum dose of 75μM/ml, showed by optimum result in the expression ofRunx2 and HIF1α further study is necessary to evaluate the angiogenic and osteogenic effect ofubiquione.

Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats Gunawan Oentaryo; Istiati Istiati; Pratiwi Soesilawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 3 (2016): September 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

Effects of liquid ionic silver concentration on caspase-3 and p53mt expressions in the oral mucosal epithelium of Wistar rats R. Aries Muharram; I. Istiati; Pratiwi Soesilawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 3 (2018): September 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Silver, especially oxidized silver, has been used as a medicine considered to have bactericidal properties. In the present day, ionic silver (Ag+) is also used in the manufacture of cosmetics, socks, food containers, detergents, sprays and other products to prevent the spread of germs. Unfortunately, ionic silver is assumed to be toxic not only to bacteria, but also to humans and the environment. Therefore, it is essential to identify the optimum dosage of ionic silver considered safe by investigating the effects of ionic silver concentration on cell death through activation of mutant p-53 expression by caspase 3 in the oral epithelium. Purpose: This research aimed to analyze the effects of concentrated liquid ionic silver (Ag+) on caspase-3 and mutant p53 expression in the oral mucosal epithelium. Methods: This research constituted a laboratory-based experimental study with posttest-only design. The research subjects consisted of 28 Wistar rats, divided into four treatment groups, namely; KK (with Aquadest), KP 1 (with 5% liquid ionic silver), KP 2 (with 10% liquid ionic silver) and KP 3 (with 15% liquid ionic silver). Each rat was then treated orally with 0.5ml of liquid ionic silver at fixed concentrations twice a day for seven days. The Wistar rats were then terminated and their tissue samples processed by means of histopathological and immunohistochemical staining examination. The monoclonal Caspase-3 and mutant p53 expressions in each group were evaluated with the data being tabulated and analyzed statistically. Results: Mutant p53 expression was also found in the control group. Moreover, the higher the concentration of liquid ionic silver, the greater the elevated Caspase-3 and mutant p53 expressions. Conclusion: The concentration of liquid ionic silver plays an important role in elevating Caspase-3 and mutant p53 expressions.

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