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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Veteriner Jurnal Ilmu Nutrisi dan Teknologi Pakan

R Misrianti

Unknown Affiliation

Author-ID : 320763

Agriculture, Biological Sciences & Forestry Veterinary

Published : 5 Documents Claim Missing Document

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Articles

Genetic Polymorphism of the Lactoferrin Gene in Dairy and Beef Cattles at National Artificial Insemination and Embryo Transfer Stations Anggraeni, Anneke; Mumpunie, G.E; Misrianti, R; Sumatri, C
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Lactoferrin (LTF) adalah gen pengontrol komponen protein susu dan memiliki karakteristik sebagai antimikrobial.Â LTF pada susu berfungsi untuk mencegah diare, sedangkan pada sapi laktasi untuk mencegah mastitis pada ambing. Mempertimbangkan peran penting dari gen LTF, maka perlu dilakukan peningkatan kadar LTF dalam susu melalui seleksi pada taraf DNA. Polymorfisme genetik dari gen LTF diidentifikasi pada sapi perah dan potong dengan metoda Polymerase Chain Reaction - Restricsion Fragment Length Polymorphism (PCR-RFLP), dengan enzim restriksi EcoRI. Genotyping dilakukan pada sapi perah Friesian Holstein (FH) total sejumlah 89 ekor, meliputi dari Balai Inseminasi Buatan Lembang (BIB Lembang) untuk 17 pejantan, Balai Besar IB Singosari (BBIB Singosari) untuk 32 pejantan, dan Balai embrio Transfer Cipelang (BETÂ Cipelang) pada 40 dara. Genotyping dilakukan pula pada sapi potong dara berasal dari empat bangsa, meliputi Limousin (14 ekor), Angus (5 ekor), Simmental (13 ekor) dan Brahman (5 ekor) dari BET Cipelang. Gen LTF|EcoRI pada sapi perah dan potong pengamatan menghasilkan dua tipe alel, yaitu alel A dan B. Kedua jenis sapi tersebut menghasilkan hanya dua genotipe, yaituÂ genotipe AA dan AB, tanpa genotipe BB. Ini dapat menjadi hal yang baik karena genotipe AA dan AB dipertimbangkan berasosiasi dengan ketahan pada mastitis. Nilai-nilai dari heterozygositas observasi (Ho) dari gen ini lebih tinggi dibandingkan heterozigositas ekspektasi (He). Disimpulkan bahwa gen LTF|EcoRI memiliki variasi yang baik pada sapi perah dan sapi potong dari ketiga balai bibit nasional tersebut. Kata Kunci: Polymorfisme genetik, Friesian Holstein, gen Lactoferrin, PCR-RFLP

Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows R Misrianti; C Sumantri; A Farajallah
Media Peternakan Vol. 33 No. 3 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Pit-1 gene has been identified as the pituitary specific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total of 320 Indonesian buffaloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH cattle. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buffaloes.

Growth Hormone Gene Polymorphism and Its Association with Partial Cumulative Milk Yields of Holstein Friesian Dairy Cattle R Misrianti; A Anggraeni; E Andreas; C Sumantri
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Growth hormone gene (GH gene) plays an important role in regulating body growth and in developing mammary gland, similar with its interaction to specific receptors. The GH gene has been considered as one of candidate gene associated with selection on lactation trait and milk production. This study was aimed to determine genetic polymorphism of the GH-AluI gene and to associate its genotype variants on various 15-d partial cumulative milk yields in Holstein Friesian (HF) dairy cows. A number of 370 blood samples were collected from six HF populations, respectively from small dairy farmer under the supervision of the North Bandung Milk Cooperation (NBMC) in Cilumber (98) and Pasir Kemis village (96), Dairy Cattle Breeding and Improvement Station (Cikole DCBIS) Cikole (88), Lembang Artificial Insemination Center (Lembang AIC) (17), Singosari Artificial Insemination Center (Singosari AIC (32), and Cipelang Livestock Embryo Center (Cipelang LEC) (40). A polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used to identify variant genotypes of the GH gene using AluI restriction enzyme. Genotyping results produced only two genotypes, i.e. LL and LV genotypes, without VV genotype. Frequency of the former was dominant, whilst that was low for the latter (89% vs. 11%); leading to the frequency of L allele was very high (94%) compared to that of V allele (6%). No significant association between variant genotypes (LL and LV) and various 15-d partial cumulative milk yields.

Genetic Polymorphism of the Lactoferrin Gene in Dairy and Beef Cattles at National Artificial Insemination and Embryo Transfer Stations Anneke Anggraeni; G.E Mumpunie; R Misrianti; C Sumatri
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Lactoferrin (LTF) adalah gen pengontrol komponen protein susu dan memiliki karakteristik sebagai antimikrobial. LTF pada susu berfungsi untuk mencegah diare, sedangkan pada sapi laktasi untuk mencegah mastitis pada ambing. Mempertimbangkan peran penting dari gen LTF, maka perlu dilakukan peningkatan kadar LTF dalam susu melalui seleksi pada taraf DNA. Polymorfisme genetik dari gen LTF diidentifikasi pada sapi perah dan potong dengan metoda Polymerase Chain Reaction - Restricsion Fragment Length Polymorphism (PCR-RFLP), dengan enzim restriksi EcoRI. Genotyping dilakukan pada sapi perah Friesian Holstein (FH) total sejumlah 89 ekor, meliputi dari Balai Inseminasi Buatan Lembang (BIB Lembang) untuk 17 pejantan, Balai Besar IB Singosari (BBIB Singosari) untuk 32 pejantan, dan Balai embrio Transfer Cipelang (BET Cipelang) pada 40 dara. Genotyping dilakukan pula pada sapi potong dara berasal dari empat bangsa, meliputi Limousin (14 ekor), Angus (5 ekor), Simmental (13 ekor) dan Brahman (5 ekor) dari BET Cipelang. Gen LTF|EcoRI pada sapi perah dan potong pengamatan menghasilkan dua tipe alel, yaitu alel A dan B. Kedua jenis sapi tersebut menghasilkan hanya dua genotipe, yaitu genotipe AA dan AB, tanpa genotipe BB. Ini dapat menjadi hal yang baik karena genotipe AA dan AB dipertimbangkan berasosiasi dengan ketahan pada mastitis. Nilai-nilai dari heterozygositas observasi (Ho) dari gen ini lebih tinggi dibandingkan heterozigositas ekspektasi (He). Disimpulkan bahwa gen LTF|EcoRI memiliki variasi yang baik pada sapi perah dan sapi potong dari ketiga balai bibit nasional tersebut. Kata Kunci: Polymorfisme genetik, Friesian Holstein, gen Lactoferrin, PCR-RFLP

Produksi Bahan Kering dan Kadar Nutrien Indigofera zollingeriana di Lahan Gambut Berdasarkan Umur Panen Berbeda Setelah Pemangkasan: Dry Matter Production and Nutrient Content of Indigofera zollingeriana on Peatland Area Based on Different Harvesting Age after Trimming Dr. Arsyadi Ali, S.Pt., M.Agr.Sc; R Artika; R Misrianti; E Elviriadi; M M Poniran
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 19 No. 2 (2021): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.19.2.30-35

This study aimed to determine the increase of dry matter production and Nutrient changes of Indigofera zollingeriana cultivated in Sapric peat soils with harvest age of 2, 3 and 4 months after pruning. Indigofera foliage was obtained from the existing plot of approximately 3 years old that grown in the experimental field of the UARDS Laboratory of the Faculty of Agriculture and Animal Sciences of UIN Suska Riau. The research design of randomized block design (RBD) was apllied consisting of 3 age treatments with 4 group. Each treatment, consisted of indigofera biomass harvested at 2 months old (2M), 3 months old (3M) and 4 months old (4M). The results showed that postponing harvest from 2 months to 4 months significantly (p<0.05) increased dry matter production of 71.97 g to 437.62 g and significantly (p<0.05) decreased crude protein (CP) content from 27.45% to 21.23%. Meanwhile, crude fibre (CF), extract ether (EE), ash and nitrogen free extract (NFE) contents were not influence by postponing harvest from 2 to 4 months. The respective ranges of CF, EE, ash and NFE content of I. zollingeriana were CF: 15.50%-16.50%, EE: 2.11%-2.85%, ash: 7.55%-8.31% and NFE: 46.39%-51.15%. It can be concluded that the dry matter production of I. zollingeriana that grown on Sapric peat soils were significantly influenced by harvest age. Key words: legume, maturity, nutritive value, peatland

Co-Authors A Anggraeni ACHMAD FARAJALLAH Anneke Anggraeni Anneke Anggraeni C Sumantri C Sumatri E Andreas E Elviriadi G.E Mumpunie M M Poniran R Artika

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