Siwi Pramatama Mars Wijayanti
Department Of Public Health, Faculty Of Health Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno 63, Purwokerto

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Staphylococcus aureus and Pseudomonas aeruginosa in Tubotympanic Chronic Suppurative Otitis Media Patients in Purwokerto, Indonesia Daniel Joko Wahyono; Anton Budhi Darmawan; Leader Alfason; Reinhard Simbolon; Siwi Pramatama Mars Wijayanti; Wisiva Tofriska Paramaiswari; Korrie Salsabila; Dodi Safari
The Indonesian Biomedical Journal Vol 12, No 4 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i4.1218

Abstract

BACKGROUND: Chronic Suppurative Otitis Media (CSOM) causes hearing impairment and frequently occurred in low-income country where medical care and personal hygiene are poor. Staphylococcus aureus and Pseudomonas aeruginosa are the most common cause of CSOM. We investigated prevalence and antimicrobial susceptibility of S. aureus and P. aeruginosa from tubotympanic CSOM patients in tertiary hospital, Purwokerto, Indonesia in 2016-2017.METHODS: Ear swab specimens were collected from patients with tubotympanic CSOM. S. aureus and P. aeruginosa were isolated and identified by culture, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and molecular tools. Antimicrobial susceptibility testing was performed using the disk diffusion method.RESULTS: Out of ear swabs from 34 patients with tubotympanic CSOM, P. aeruginosa and S. aureus were identified in 35%patients. No Methicillin-resistant S. aureus (MRSA) strain was found from the ear swabs of the patients with tubotympanic CSOM. Bacterial identification using the MALDI-TOF MS was concordantly with culture and molecular tools. All S. aureus isolates showed full susceptibility to cefoxitin and trimethoprim-sulphamethoxazole. Resistance to tetracycline was common with only 64% of S. aureus strains being susceptible. Meanwhile, all P. aeruginosa strains were susceptible to cefepime, cetazidime, meropenem, gentamicin, and tobramycin.CONCLUSION: S. aureus and P. aeruginosa are found in patients with tubotympanic CSOM and still susceptible to different antibiotic agents. MALDI-TOF MS demonstrate rapid, accurate and robust to detect S. aureus and P. aeruginosa.KEYWORDS: Staphylococcus aureus, Pseudomonas aeruginosa, chronic tubotympanic suppurative otitis media
Seroprevalence of Japanese enchephalitis Infection in Pigs in Tulungagung, East Java Dyah Widiastuti; Tri Wijayanti; Tri Isnani; Nova Pramestuti; Siwi Mars Pramatama Wijayanti
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 15 Nomor 2 Desember 2019
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.08 KB) | DOI: 10.22435/blb.v15i2.1888

Abstract

Pigs play a role as amplifier hosts for Japanese enchephalitis (JE). Surveillance of JEV infection on pigs, is, therefore undoubtedly important to prevent its transmission to humans. This study aims to investigate the infection rates of JE in pigs as a risk of JE in human. The study area was located in Tulungagung regency, one of the regencies in East Java with the largest number of pig farms. This was a cross sectional study, involving five pig farms in Tulungagung Regency during April-November 2016, with a total of 63 pigs examined. Venous blood was examined by JEV specific IgG-Enzyme Link Immuno-Sorbent Assay (ELISA). The results showed that the JE infection rates in overall pig samples was 19.05% (12/63). The prevalence of Ab JE in 4-6 month pigs is higher (22.7%) than in 2-3 month pigs. The infections in pigs tend to be higher (23.1%) in farms found to be positive for Culex larvae. Sex and age of the pig and the presence of Culex larvae surround pig farms were not significantly associated with JE infection rates in pigs. In conclusion, the presence of JE virus antibodies in pigs population in Tulungagung indicates that there is a potency of JE virus transmission to humans.
EKSPLORASI POTENSI EKSTRAK CAIR DAUN KECOMBRANG YANG MENGANDUNG ANTIOKSIDAN SEBAGAI PENETRALISIR RADIKAL BEBAS DALAM DARAH PETUGAS SPBU Azzah Farah Fadiyah; Resi Mukti Wardhani; Nurmei Rahmatika; Siwi Pramatama Mars Wijayanti
JURNAL LITBANG KOTA PEKALONGAN Vol. 15 (2018)
Publisher : Badan Perencanaan Pembangunan, Penelitian dan Pengembangan Daerah (Bappeda) Kota Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54911/litbang.v15i0.72

Abstract

ABSTRAK Pekerjaan sebagai petugas Stasiun Pengisian Bahan Bakar Umum (SPBU) memiliki risiko akibat paparan radikal bebas di lingkungan SPBU yang dapat menyebabkan modifikasi lipid, DNA, dan protein pada berbagai jaringan tubuh. Untuk menetralisir radikal bebas tersebut tubuh memiliki antioksidan alami yaitu Superoksida Dismutase (SOD). Daun kecombrang memiliki kandungan antioksidan terutama flavonoid yang mampu menetralisir radikal bebas. Oleh karena itu, bertujuan mengeksplorasi potensi ekstrak cair daun kecombrang sebagai penetralisir radikal bebas dalam darah. Metode penelitian ini merupakan penelitian eksperimen yang diujikan pada tikus. Langkah yang dilakukan dalam penelitian ini antara lain pembuatan ekstrak daun kecombrang, perlakuan terhadap hewan uji dan pemeriksaan kadar SOD serum darah hewan uji. Hewan uji berupa tikus jantan galur Wistar sebanyak 16 ekor yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol positif yang diinduksi CCL4 1mL/KgBB dan placebo berupa Na-CMC 1%, kelompok perlakuan 1 yang diinduksi CCL4 1mL/KgBB dan ekstrak daun kecombrang 100mg/KgBB, kelompok perlakuan 2 yang diinduksi CCL4 1mL/KgBB dan ekstrak daun kecombrang 150mg/KgBB, dan kelompok perlakuan 3 yang diinduksi CCL4 1mL/KgBB dan ekstrak daun kecombrang 200mg/KgBB. Masing-masing kelompok terdiri dari 4 ekor tikus. Berdasarkan hasil eksperimen pengukuran kadar SOD didapati hasil bahwa ekstrak daun kecombrang dapat meningkatkan kadar SOD serum darah tikus yang diinduksikan CCL4 sebagai radikal bebas. Berdasarkan hasil eksperimen juga diketahui bahwa dosis optimal ekstrak cair daun kecombrang untuk meningkatkan kadar SOD serum darah pada subjek penelitian adalah 150mg/KgBB. Dari penelitian ini diketahui bahwa ada potensi untuk mengembangkan ekstrak cair daun kecombrang sebagai penetralisisr radikal bebas. Kata Kunci: Ekstrak cair daun kecombrang, Radikal bebas, Antioksidan, SOD, In Vivo
Factors Affecting the Quality of Life of Breast Cancer Patients Alifka Deti Sri Maharani; Dwi Sarwani Sri Rejeki; Siwi Pramatama Mars Wijayanti
Disease Prevention and Public Health Journal Vol. 17 No. 2 (2023): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v17i2.8705

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Background: Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer death among women worldwide in 2020. This systematic review aims to identify the factors that affect the quality of life of breast cancer patients so that the results of this systematic ventilation can help health professionals develop appropriate interventions. Method: This systematic review followed the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. This study aims to identify the factors that affect the quality of life of breast cancer patients. A comprehensive search was performed across several databases, including PubMed, Embase, Scopus, and Web of Science. The search was limited to studies published between 2019 and 2022, which were written in English. Results: Factors that affect the quality of life of breast cancer patients include age, education level, social support, psychological stress and anxiety, economic status, depression, and physical symptoms. Conclusion: Several factors can affect the quality of breast cancer patients, so the patient needs support from the family and health policies from the government in their treatment.
Malaria Infection Among Pregnant Women of Abau District in Papua New Guinea Susina Yatapya; Dwi Sarwani Sri Rejeki; Siwi Pramatama Mars Wijayanti; Radi Radi
Jurnal Kesehatan Masyarakat Vol. 20 No. 4 (2025)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v20i4.10714

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Malaria is a communicable disease that poses a serious problem in Papua New Guinea, with the country recording the highest incidence of malaria in the Asia-Pacific region each year. However, research on the risk factors for malaria in Papua New Guinea is still minimal, especially among pregnant women. This study aims to investigate the correlation between malaria incidence in pregnant women in Papua New Guinea. This study is an observational study with a cross-sectional approach conducted on 200 pregnant women from January to March 2024 in Abou District, Papua New Guinea. Data collection used questionnaires and Rapid Diagnostic Tests (RDT) to diagnose malaria infection in pregnant women. The independent variables in this study are healthcare-seeking behavior, prevention of malaria, knowledge of malaria, maternal age, marital status, occupation, education level, and residential location. The dependent variable in this study is the incidence of malaria in pregnant women. In this study, the data analysis used included univariate analysis, bivariate analysis with chi-square, and multivariate analysis with logistic regression. The results showed that the risk factors for malaria incidence in pregnant women in Papua New Guinea are malaria prevention (p-value 0.014; OR= 4.426) and healthcare-seeking behavior (p-value 0.033; OR= 4.033), meaning that pregnant women with poor malaria prevention behavior and poor healthcare-seeking behavior are four times more likely to suffer from malaria. It is hoped that pregnant women will increase their awareness of malaria prevention during pregnancy and make full use of healthcare facilities during pregnancy.
Analisis Faktor Risiko Kejadian Diabetes Mellitus Tipe II di Wilayah Pedesaan Siwi Pramatama Mars Wijayanti; Tias Tri Nurbaiti; Arrum Firda Ayu Maqfiroch
Jurnal Promosi Kesehatan Indonesia Volume 15, No. 1 Januari 2020
Publisher : Master Program of Health Promotion Faculty of Public Health Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jpki.15.1.16-21

Abstract

Background: Diabetes mellitus (DM) is a non-communicable disease that remains a significant health concern in Indonesia. The disease is more prevalent in urban areas due to lifestyles, socioeconomics level and lack of physical activity. Currently, DM has begun occured also in rural areas. The purpose of this study is to identify the risk factors associated with Type II DM incidence in rural areas.Method: This is analytical study with a case-control design, located in Community Health Centre 1, Wangon, Banyumas Regency. There are 65 people in case group and 65 people in control group involved in this study. Variables included personal characteristics, dietary habit, physical activity and exposure to cigarette smoke. Data was obtained by structured questionnaire and analyze using univariate, bivariate (chi-square test) and multivariate analysis (logistic regression test).Results: The results showed that the dietary habit (OR = 11,824; 95% CI = 4,988-28,032) and physical activity (OR = 2,608; 95% CI = 1,116–6,095) had influenced on the incidence of DM type II. Whilst the exposure of cigarette smoking did not influenced the incidence of DM type II. It is suggested that consume foods with balanced nutrition and doing physical activities should be conducted routinely in accordance with WHO recommendations.
Analisis hubungan pelayanan antenatal care (ANC) terpadu dengan angka kematian ibu Salsa Adi Aldila; Dwi Sarwani Sri Rejeki; Siwi Pramatama Mars Wijayanti; Paramita Adi Nurmutia
Hik Vol. 19 No. 3 (2025): Volume 19 Nomor 3
Publisher : Program Studi Ilmu Keperawatan-fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/hjk.v19i3.926

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Background: Maternal Mortality Rate (MMR) is still a global health problem, including in Indonesia. One effort to reduce the risk of maternal death is through integrated antenatal care (ANC) health services according to standards. Purpose: To analyze the relationship between integrated antenatal care (ANC) services and maternal mortality rates. Method: Quantitative research with a correlational approach. The research sample was 40 health centers in Banyumas Regency which was implemented in April 2024. The data analysis used was the Spearman Rank Correlation test to test the relationship between integrated ANC service variables which include 10 integrated examinations with maternal mortality variables. Results: This study found that weight measurement (p value = 0.005), blood pressure measurement (p value = 0.005), upper arm circumference measurement (p value = 0.017), uterine fundus height measurement (p value = 0.012), iron tablet administration (p value = 0.012), pregnancy examination (p value = 0.005), hemoglobin examination (p value = 0.011), blood type examination (p value = 0.011), urine glycoprotein examination (p value = 0.016) and consultation/counseling (p value = 0.014) were significantly associated with maternal mortality. Conclusion: Integrated ANC services that are of high quality and meet standards are one of the efforts to reduce high maternal mortality rates. The quality of ANC services that do not meet standards are associated with maternal mortality. Suggestion: There needs to be periodic competency improvement for health workers who carry out integrated ANC services in health centers to strengthen service quality assurance.   Keywords: Health Services; Integrated Antenatal Care (ANC); Maternal Mortality Rate (MMR).   Pendahuluan: Angka kematian ibu (AKI) masih menjadi isu masalah kesehatan di tingkat global, termasuk di Indonesia. Salah satu upaya untuk mengurangi risiko kematian ibu adalah melalui pelayanan kesehatan maternal antenatal care (ANC) terpadu sesuai standar. Tujuan: Untuk menganalisis hubungan pelayanan antenatal care (ANC) terpadu dengan angka kematian ibu.  Metode: Penelitian kuantitatif dengan pendekatan korelasional. Sampel penelitian terdiri dari 40 puskesmas di Kabupaten Banyumas yang dilaksanakan pada bulan April 2024. Analisis data yang digunakan adalah uji Korelasi Rank Spearman untuk menguji hubungan antara variabel pelayanan ANC terpadu yang mencakup pemeriksaan 10 terpadu dengan variabel angka kematian ibu. Hasil: Penelitian ini menemukan bahwa pengukuran berat badan (p value = 0.005), pengukuran tekanan darah (p value = 0.005), pengukuran lingkar lengan atas (p value = 0.017), pengukuran tinggi fundus uterus (p value = 0.012), pemberian tablet tambah darah (p value = 0.012), tes kehamilan (p value = 0.005), pemeriksaan hemoglobin (p value = 0.011), pemeriksaan golongan darah (p value = 0.011), pemeriksaan glukoproterin urin (p value = 0.016) dan temu wicara/konseling (p value = 0.014) berhubungan secara signifikan dengan angka kematian ibu. Simpulan: Pelayanan ANC terpadu yang berkualitas dan sesuai standar merupakan salah satu upaya untuk menurunkan tingginya angka kematian ibu. Kualitas pelayanan ANC yang belum memenuhi standar memiliki hubungan dengan angka kematian ibu. Saran: Perlunya peningkatan kompetensi secara berkala bagi tenaga kesehatan yang melaksanakan pelayanan ANC terpadu di puskesmas untuk memperkuat jaminan mutu pelayanan.   Kata Kunci: Angka Kematian Ibu; Antenatal Care (ANC) Terpadu; Pelayanan Kesehatan.
Analisis kategori wilayah tempat tinggal dengan status calon pengantin wanita terhadap risiko melahirkan anak stunting Ananda Bela Yustisia; Siwi Pramatama Mars Wijayanti; Dwi Sarwani Sri Rejeki; Fauzia Annafiun Nisa; Salsa Adi Aldila
Hik Vol. 19 No. 7 (2025): Volume 19 Nomor 7
Publisher : Program Studi Ilmu Keperawatan-fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/hjk.v19i7.969

Abstract

Background: Stunting in Indonesia still has a high prevalence at 21.5% in 2023. Stunting is also caused by the mother's condition before marriage. Prospective brides still have low awareness about the importance of preventing stunting at a young age. Premarital health conditions can be influenced by various factors, including the type of ​​residence which may influence physical, social and economic environmental conditions. Purpose: To determine whether the regional category affects the status of prospective brides who run the risk of having children who are stunted. Method: A quantitative analytical study used a cross-sectional method and was conducted in June-July 2024 in Banyumas Regency, Central Java. The sampling technique used total sampling with a sample size of 3.399 respondents. The independent variables in this study were rural and urban areas of residence, while the dependent variable was stunting. Data analysis used univariate in the form of frequency distribution and bivariate using chi-square. Results: The chi-square test results showed a p-value of 0.216 (>0.050). This means that there is no significant relationship between rural and urban areas and the risk of stunting in prospective brides. Conclusion: The status of prospective brides by category puts them at risk of giving birth to stunted children, and this risk does not differ between rural and urban areas. Pre-marital and pregnancy education should be provided evenly across all regions, and assistance should be provided to prospective brides at risk to delay pregnancy until conditions are more ideal.   Keywords: Premarital Health; Stunting; Town; Village.   Pendahuluan: Stunting di Indonesia masih memiliki prevalensi yang tinggi, yaitu 21,5% pada tahun 2023. Stunting ikut dipengaruhi oleh kondisi ibu sejak sebelum menikah. Namun, calon pengantin wanita cenderung memiliki kesadaran yang rendah akan pentingnya pencegahan stunting sejak dini. Kondisi kesehatan pranikah dapat dipengaruhi oleh berbagai faktor, termasuk wilayah tempat tinggal yang memungkinkan adanya kondisi lingkungan fisik, sosial, dan ekonomi yang mempengaruhi. Tujuan: Untuk mengetahui apakah kategori wilayah tempat tinggal berhubungan dengan status calon pengantin wanita berisiko melahirkan anak stunting. Metode: Penelitian kuantitatif analitik menggunakan metode cross sectional, dilaksanakan pada bulan Juni-Juli 2024 di Kabupaten Banyumas, Jawa Tengah. Teknik pengambilan sampel menggunakan total sampling dengan jumlah sampel yang digunakan sebanyak 3399 responden. Variabel independen dalam penelitian ini adalah wilayah tempat tinggal perdesaan dan perkotaan, sedangkan variable dependen adalah stunting. Analisis data yang digunakan univariate dalam bentuk distribusi frekuensi dan bivariate menggunakan chi square. Hasil: Uji chi square menunjukkan p-value 0.216 (>0.050). Artinya, tidak terdapat hubungan yang signifikan antara wilayah perdesaan dan perkotaan dengan risiko stunting pada calon pengantin wanita Simpulan: Status calon pengantin wanita oleh kategori berisiko melahirkan anak stunting, dan risiko tersebut tidak berbeda antara wilayah perdesaan maupun perkotaan. Edukasi pranikah dan kehamilan secara merata di seluruh wilayah, serta pendampingan bagi calon pengantin berisiko untuk menunda kehamilan hingga kondisinya lebih ideal.   Kata Kunci: Kesehatan Pranikah; Perdesaan; Perkotaan; Stunting.
Analisis spasial terhadap kejadian demam berdarah dengue (DBD) di Indonesia: A systematic review Veronika Angellina Hiberto Ho; Siwi Pramatama Mars Wijayanti; Dwi Sarwani Sri Rejeki
Hik Vol. 19 No. 5 (2025): Volume 19 Nomor 5
Publisher : Program Studi Ilmu Keperawatan-fakultas Ilmu Kesehatan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/hjk.v19i5.1180

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Background: Dengue Hemorrhagic Fever (DHF) is a major public health problem in Indonesia, with significant regional variations in incidence. Despite the government's implementation of various control programs, DHF cases continue to exhibit specific spatial clustering patterns influenced by environmental and demographic factors. Purpose: To analyze the spatial distribution of dengue fever cases in Indonesia and identify environmental and climatic factors associated with dengue fever incidence through a spatial analysis approach. Method: This study followed the PRISMA guidelines and included articles published between January 2020 and December 2024. A literature search was conducted through the PubMed and SINTA databases using the keywords "spatial analysis," "dengue hemorrhagic fever," "DHF," and "Indonesia." Articles meeting the inclusion criteria were analyzed narratively and grouped based on similar findings. Results: Six articles met the inclusion criteria. Most studies used spatial analysis tools such as ArcGIS, Moran's I, and LISA to map clustering and autocorrelation patterns of dengue cases. The results showed that dengue cases tended to be concentrated in areas with high population density. Environmental factors such as temperature (25°C–30°C) and humidity were strongly associated with mosquito behavior and dengue transmission risk, while rainfall showed a weaker relationship. One study demonstrated a dispersed pattern of cases. Conclusion: Spatial analysis methods have proven effective in identifying high-risk dengue clusters and understanding the influence of environmental variables on transmission dynamics. The results of this review emphasize the importance of geographically focused public health interventions and the need for continuous monitoring and mapping of dengue cases in endemic areas.   Keywords: Air Humidity; Dengue Hemorrhagic Fever; Spatial Analysis.   Pendahuluan: Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan masyarakat utama di Indonesia dengan variasi kejadian yang signifikan antar wilayah. Meskipun pemerintah telah menjalankan berbagai program pengendalian, kasus DBD tetap menunjukkan pola pengelompokan spasial tertentu yang dipengaruhi oleh faktor lingkungan dan demografis. Tujuan: Untuk menganalisis pola distribusi spasial kasus demam berdarah di Indonesia dan mengidentifikasi faktor lingkungan dan iklim yang terkait dengan kejadian demam berdarah melalui pendekatan analisis spasial. Metode: Penelitian tinjauan pustaka menggunakan pedoman PRISMA dan mencakup artikel yang diterbitkan pada periode Januari 2020 hingga Desember 2024. Pencarian literatur dilakukan melalui database PubMed dan SINTA dengan kata kunci "analisis spasial", "demam berdarah dengue", "DBD" dan “Indonesia”. Artikel yang memenuhi kriteria inklusi dianalisis secara naratif dan dikelompokkan berdasarkan kesamaan hasil. Hasil: 6 artikel memenuhi kriteria inklusi dan sebagian besar penelitian menggunakan alat analisis spasial, seperti ArcGIS, Moran's I, dan LISA untuk memetakan pola pengelompokan dan autokorelasi kasus demam berdarah. Kasus demam berdarah cenderung terkonsentrasi di daerah dengan kepadatan penduduk tinggi. Faktor lingkungan seperti suhu (25–30°C) dan kelembaban udara memiliki hubungan yang kuat dengan perilaku nyamuk dan risiko penularan demam berdarah, sedangkan curah hujan menunjukkan hubungan yang lemah. Simpulan: Metode analisis spasial telah terbukti efektif dalam mengidentifikasi klaster demam berdarah berisiko tinggi dan memahami pengaruh variabel lingkungan terhadap dinamika penularan. Hasil tinjauan ini menekankan pentingnya intervensi kesehatan masyarakat yang berfokus secara geografis dan perlunya pemantauan dan pemetaan kasus demam berdarah secara berkelanjutan di daerah endemik.   Kata Kunci: Analisis Spasial; Demam Berdarah Dengue; Kelembaban Udara.