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All Journal MARINA CHIMICA ACTA Indonesia Chimica Acta Warta LPM
Ismail Marzuki
Universitas Fajar Makassar
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Engineering Health Professions Social Sciences

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MICROSYMBIONT AND MORPHOLOGICAL PHENOTYPE ANALYSIS Ismail Marzuki
Marina Chimica Acta Vol. 17 No. 1 (2016): Radioactivity, Sponge Microsymbiont to degrade sludge oil, Honey as part of Sp
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.116 KB) | DOI: 10.20956/mca.v17i1.932

Abstract

 Determination biomass and phenotypic analysis of microsymbionts sponge is a comprehensive effort to discover the specificity of the sponge, not only on the identification and characterization studies that have been growing. Research directed at diversification of knowledge of the functions and benefits of a sponge for the life and welfare of mankind. The purpose of this research is the analysis of biomass morphology and phenotype test microsymbionts sponge. Histomorfologi analysis method to determine the type, components and composition biomass. Isolates obtained by sponge microsymbiont isolation-purification followed by phenotypic analysis through Gram staining and biochemical tests. Histomorfologi analysis results obtained sponge species is Callyspongia sp. Components and composition consists of sponge biomass fraction skeleton (spicules and cell debris) reached 69.8 %, 18.8 % sponge cell fraction and 11.3 % bacterial pellet fraction. The results of the isolation-purification microsymbiont obtained two isolates. Staining test results both isolates are Gram-positive bacteria and biochemical tests is Bacillus subtilis isolates the spherical shape large size, beige and white, while the isolates two clustered colonies are bacillus flexus jagged shape elongated, white-purple color and a separate colony. Keywords: histomorfologi, phenotype, Callyspongia sp, biomass, microsymbiont, sponge 
MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS Ismail Marzuki; Alfian Noor; Nursiah La Nafie; M Natsir Djide
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.284 KB) | DOI: 10.20956/mca.v16i1.956

Abstract

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules
THE POTENTIAL BIODEGRADATION HYDROCARBONS OF PETROLEUM SLUDGE WASTE BY CELL BIOMASS SPONGE Callysppongia sp. Ismail Marzuki; Alfian Noor; Nursiah La Nafie
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.121 KB) | DOI: 10.20956/mca.v16i2.7407

Abstract

The toxicity of petroleum sludge is a serious threat to marine life. Necessary concrete stepspetroleum contamination reduction through a search of potential marine materials degradetoxic components hydrocarbons. The sponge is one potential material reducing toxicproperties of petroleum contamination. The research objective was to determine thepotential Callyspongia sp sponge against the degradation of petroleum hydrocarbonsludge. Four variations of treatment used to determine the potential of sponge biomass,namely: the treatment I, II, III and IV. Indicators degradation is the formation of gas, thesmell fermentation, pH changes and increase in the absorbance of the suspension of mediadegradation. The level degradation each treatment is determined gravimetric method andthe known hydrocarbon component changes by using GC-MS. Unidentified gas, the smellof fermentation and changes in media of pH on average degradation occurs on the 15 daysof contact for all treatments. Data showed degradation occurs absorbance maximum at 20-25 days to contact. The highest degradation rates indicated by IV treatment (26.93 %), III(24.84 %), II (22.59 %), and I: (18.20 %). Found 20 kinds of components in the wastesludge aliphatic petroleum to form a homologous series nC10-nC30, and two aromaticcomponents, namely the 2.7-dimethyl naphthalene and 1.4-dimethyl azulene. Callyspongiasp sponge biomass concluded at the highest IV treatment degrading aliphatichydrocarbons, then treatment III, II and I, while the aromatic components are not degradedby biomass Callyspongia sp sponge at all treatments.
The Bio-Adsorption Pattern Bacteria Symbiont Sponge Marine Against Contaminants Chromium and Manganese In The Waste Modification of Laboratory Scale Ismail Marzuki
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 13, No 1: June 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v13i1.9972

Abstract

The use of sponge symbionts bacteria as marine biomaterials in the heavy metal bio-adsorption method is an effort to save the marine environment from contamination of heavy metal contaminants. The ocean is a giant container, most vulnerable to contamination of pollutants. The target of the research is to determine the potential, capacity and pattern of bio-adsorption of sponge symbionts bacteria against various pollutants so that the toxic properties of heavy metal contaminants can be minimize. The method used is to interact with the bacterial suspension on the test metal concentrations that have been determined. The parameters measured were optical density, pH and concentration of heavy metals after the interaction lasted several days and the calculation of capacity, efficiency and bio-adsorption patterns of bacterial isolates from sponges. Results: The pattern and bio-adsorption power of AC bacteria to Cr and Mn ions were higher than AC bacteria, the adaptability of AC and BS bacteria was stronger in Cr (III) contaminated media compared to Cr (VI) toxic media, causing bacterial cell population BS and AC in Cr (III) and Mn (II) media are more abundant than in Cr (VI) and Mn (VII) media, capacity and bio-adsorption efficiency of BS and AC bacteria agains Cr (III) ˃ Cr (VI) ions and Mn (II) ˃ Mn (VII), It is suspected that there is an influence of reactivity and toxic properties of the metal ion test on the performance of the sponge symbionts in bio-adsorption
Performance Analysis of biosorption of Heavy Metal and Biodegradation PAH of Isolates Marine Sponges Symbiont Bacteria Ismail Marzuki; Andi Muhammad Asdar Marzuki; Hardimas Hardimas
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 14, No 2: December 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v14i3.18333

Abstract

Heavy metal pollutants and polycyclic aromatic hydrocarbon contaminants, also known as PAHs, need attention from related parties, considering that their use is very wide, as well as their relatively high natural releases. Careless and uncontrolled handling has the potential to cause problems for creatures, especially in marine ecosystems. The aim of the study was to determine the performance of marine sponge microsymbiont isolates in heavy metal biosorption and PAH biodegradation. The method applied, pure isolates of marine sponge symbionts were cultured on NA media. The suspension of isolates was made using physiological 0.9 % NaCl solution. Suspension adapted 24 hours. The isolate suspension was interacted with heavy metals Cd2+ and As3+, each with a concentration of 100 ppm and naphthalene and anthracene with a concentration of 1000 ppm. Interaction time of 5, 10, 15, and 20 days. Results of the analysis showed the biosorption capacity of the bacterial isolate Sp6.B2 to Cd2+ = 83.190 %, while Sp8.B1 = 82.240 %. Biosorption performance of Sp6.B2 isolates against As3+ = 99.890 %, while Sp8.B1 = 99.894 %. The biodegradation performance of Sp6.B2 isolates had a higher aggressiveness towards naphthalene and anthracene test contaminants than Sp8.B1 isolates. These results indicate that the biosorption performance of Sp6.B2 ˃ Sp8.B1 isolates against Cd2+ contaminants and vice versa Sp6.B2 ˂ Sp8.B1 against As3+ biosorption, while the biodegradation performance of Sp6.B2 ˃ Sp8.B1 isolates both against naphthalene and anthracene test contaminants
ISM Identification and Distribution of Polycyclic Aromatic Hydrocarbon Pollutants in Coastal Ecosystem the Marine Tourism Area, South Sulawesi Ismail Marzuki; Nur Rahmi Putri Lubis; Irham Pratama; Noviar Nurdin Kasim
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 15, No 1: June 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v15i1.20898

Abstract

Today's marine tourism is experiencing rapid growth to meet the global needs of the world's population. Exploitation of marine biological wealth is no less important because it involves human life. The marine ecosystem must be of high quality and free from exposure to toxic pollutants, such as PAHs. The marine ecosystem must be of high quality and free from exposure to toxic pollutants, such as PAHs, microplastics. This research aims to provide data and information about the types and abundance of aromatic hydrocarbon compounds in sediments, sea water and marine biota around KKI waters. The analytical method to meet these objectives uses GS/MS. The status of marine tourism areas, especially around KKI waters, is declared polluted by PAHs. The types of PAHs identified in each sample at the three sampling stations were dominated by naphthalene (NL), phenanthrene (PT), pyrene (PR) and azulene (AZ). The average total abundance of PAHs in sponge samples (±70.51%), sediments (±67.30%), followed by seawater samples (±64.85%), starfish samples (±41.80%) and fish (±26.74%). The NL type PAHs were found in all types of samples and at all stations, where the PAHs were thought to originate from industrial, hospital, and household activities. The status of marine tourism, especially around the KKI waters, for tourists needs to be careful and alert, because the KKI area is not completely free from harmful and toxic pollutants, so it is a risk to health. Makassar City TMA managers are encouraged to make efforts to reduce the rate of increase in the concentration of regional PAHs, such as providing periodic data and information about harmful pollutants, education for every tourist to care about waste and planting coastal plants such as mangroves which have a biofilter function against toxic pollutants.
Analisis Mutu Air Berdasarkan Indeks Pencemaran pada Outlet Limbah Cair Pasar Terong Kota Makassar Ritnawati Makbul; Natsar Desi; Ismail Marzuki
Warta LPM WARTA LPM, Vol. 25, 2022: Special Issue
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (819.853 KB)

Abstract

Penelitian ini bertujuan untuk mengkaji kualitas air outlet Pasar Terong Kota Makassar dari aktivitas perdagangan dan kantor Pasar Terong Kota Makassar berdasarkan parameter Indeks Kualitas Air (IKA). Pengambilan sampel dilakukan di tiga titik dengan 3 kali ulangan dalam interval waktu satu bulan dengan metode contoh sesaat (Grab Sample) atau pengambilan sampel air yang mempunyai karakteristik air tidak berubah dan mewakili keadaan waktu dan tempat tersebut. Kualitas air dianalisis secara deskriptif berdasarkan acuan Baku Mutu Air. Hasil uji laboratorium menunjukkan bahwa kualitas air pada outlet Pasar Terong Kota Makassar melebihi Baku Mutu Air Kelas II berdasarkan Peraturan Pemerintah Nomor 82 Tahun 2001 dengan parameter antara lain TSS (106.72 mg/l), BOD (52.05 mg/l), COD (132.95 mg/l), pH (7.18), Salinitas (33 mg/l) dan Suhu (31.80C). Hasil penelitian dapat disimpulkan bahwa kondisi kualitas limbah cair Pasar Terong Kota Makassar berada pada status tercemar sedang. Limbah cair Pasar Terong Kota Makassar dihasilkan dari pasar tradisional berasal dari bermacam-macam aktivitas, baik itu dari kegiatan perdagangan, perkantoran, kamar mandi dan kios makanan. Demi meningkatkan kualitas limbah yang sesuai dengan baku mutu yang diperbolehkan dalam arti parameter limbah cair Pasar Terong Kota Makassar tidak melebihi baku mutu yang ditentukan, diharapkan Pasar Terong Kota Makassar terdapat pengolahan atau instalasi pengolahan air limbah (IPAL) ataupun sistem yang digunakan dalam penanganan limbah cair Pasar Terong Kota Makassar di masa mendatang.
Co-Authors Alfian Noor Andi Muhammad Asdar Marzuki Desi, Natsar Hardimas Hardimas Irham Pratama M Natsir Djide Noviar Nurdin Kasim Nur Rahmi Putri Lubis Nursiah La Nafie Ritnawati Makbul