Garuda - Garba Rujukan Digital

Eddy Suprayitno

Unknown Affiliation

Author-ID : 3229857

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Materials Science & Nanotechnology Physics

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Articles

1

Pengaruh Sipermetrin Pada Jambal Rotiterhadap Kadaru Reum dan Kreatinin Tikus Wistar (Rattus norvegicus) Nursinah Amir; Eddy Suprayitno; Hardoko Hardoko; Happy Nursyam
Jurnal IPTEKS Pemanfaatan Sumberdaya Perikanan Vol. 2 No. 3 (2015)
Publisher : Hasanuddin University

Sipermetrin pada dasarnya digunakan untuk mengendalikan hama, penyakit dan gulma pengganggu pada kegiatan pertanian. Tetapi oleh pengolah Jambal Roti digunakan untuk mencegah pembusukan sehingga daya simpan produk lebih lama dan kerugian bisa dikurangi. Penelitian ini bertujuan untuk mengetahui pengaruh sipermetrin terhadap kadar ureum dan kreatininTikus Wistar (Rattus norvegicus). Tikus diberi perlakuan paparan sipermetrin dengan dosis 0.00 mg/kg (K-), 0.05 mg/kg, 0.60 mg/kg, 1.10 mg/kg, 1.60 mg/kg, 2.15 mg/kg dan daging ikan yang mengandung sipermetrin 1.73 mg/kg (kontrol positif : K+). Kadar ureum dan kreatinin darah tikus ditetapkan berdasarkan metode Enzymatic Photometric. Hasil menunjukkan bahwa sipermetrin berpengaruh terhadap peningkatan kadar ureum dan kreatinin Tikus Wistar. Kadar ureum sudah melewati normal pada hari ke-7 pemeliharaan untuk perlakuan sipermetrin dosis 2.15 mg/kg. Rata-rata kadar kreatinin pada hari ke-14 semua perlakuan sudah melewati batas kadar normal, kecuali pada perlakuan K- dan 0.05 mg/kg. Kadar ureum dan kreatinin tertinggi yaitu 27.7±0.98 mg/dL dan 1.03±0.018 mg/dL diperoleh pada perlakuan sipermetrin dosis 2,15 mg/kg.

Profile of Amino Acids and Proximate on the Extraction of Red Ginger Protease Enzymes in Commercial Feed for Aquaculture Yessi Renita Efendi; Eddy Suprayitno; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.4141

Quality feed in aquaculture is very important to increase the growth and health of fish. Low feed efficiency can be overcome by feed supplementation using catalytic enzymes. One of the enzymes that can stimulate digestion is protease in red ginger plants. The protease enzyme in red ginger plants plays a role in breaking peptide bonds in place of carboxyl groups so that peptides are formed from complex compounds into simpler compounds. This study aims to determine the profile of amino acids and proximate in feed given red ginger enzymes. This research uses descriptive method. The feed sample used was commercial feed with 4 treatments namely Control (without red ginger enzyme), treatment A (1%), treatment B (3%), and treatment C (5%). The analyzes carried out included red ginger enzyme extraction, protease enzyme purification, proximate and amino acid analysis of each type of feed. The results showed that the results of the SDS-Page analysis on the red ginger protease sample contained at 67.1 kDa. The results of the proximate analysis showed the highest value in feed containing 3% red ginger protease (B) with a percentage of 42.69%. The resulting amino acid content consists of 8 non-essential amino acids namely, L-serine, L-glutamic acid, L-alanine, L-arginine, glycine, L-aspartic acid, L-tyrosine and L-proline. While the essential amino acids consist of 7 types of amino acids namely, L-Phenylalani, L-Isoleucine, L-Valine, L-Lysine, L-Leucine, L-Threonine and L-Histidine. The best type of feed is feed B, which is feed with the addition of 3% red ginger protease.

Profile of Amino Acids and Proximate on the Extraction of Red Ginger Protease Enzymes in Commercial Feed for Aquaculture Yessi Renita Efendi; Eddy Suprayitno; Anik Martinah Hariati
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.4141

Quality feed in aquaculture is very important to increase the growth and health of fish. Low feed efficiency can be overcome by feed supplementation using catalytic enzymes. One of the enzymes that can stimulate digestion is protease in red ginger plants. The protease enzyme in red ginger plants plays a role in breaking peptide bonds in place of carboxyl groups so that peptides are formed from complex compounds into simpler compounds. This study aims to determine the profile of amino acids and proximate in feed given red ginger enzymes. This research uses descriptive method. The feed sample used was commercial feed with 4 treatments namely Control (without red ginger enzyme), treatment A (1%), treatment B (3%), and treatment C (5%). The analyzes carried out included red ginger enzyme extraction, protease enzyme purification, proximate and amino acid analysis of each type of feed. The results showed that the results of the SDS-Page analysis on the red ginger protease sample contained at 67.1 kDa. The results of the proximate analysis showed the highest value in feed containing 3% red ginger protease (B) with a percentage of 42.69%. The resulting amino acid content consists of 8 non-essential amino acids namely, L-serine, L-glutamic acid, L-alanine, L-arginine, glycine, L-aspartic acid, L-tyrosine and L-proline. While the essential amino acids consist of 7 types of amino acids namely, L-Phenylalani, L-Isoleucine, L-Valine, L-Lysine, L-Leucine, L-Threonine and L-Histidine. The best type of feed is feed B, which is feed with the addition of 3% red ginger protease.

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