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Prevalence of Intestinal Parasites amongst selected age groups within Okada, South –South Nigeria. Osazee Ekundayo Izevbuwa; Obhioze Augustine Akpoka; Ebere Julein Okafor-Elenwo
International Journal of Applied Biology Vol. 4 No. 1 (2020): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v4i1.9549

Abstract

This study was conducted to determine the prevalence of intestinal parasites from volunteered individuals in the study site. A total of 300 stool samples, comprising 150 females and 150 males were collected randomly from the study population in Ovia North-East Local Government Area in Edo State, aged between 1 and 60 years. The stool samples were aseptically collected and examined using both direct smear method and formol ether sedimentation techniques to determine the individual parasite load of the sampled population. The age group of 16-20 had the highest number of participants 102(34%) while the age group of 6-10, 51-55 and 56-60 had the least number of participants of 3(1%) respectively. Out of the 300 stool samples examined 87, (29%) were positive with intestinal parasites while 213(71%) were negative, not having any intestinal parasitic infection However the age group of 11-15(N=10) and 31-35(N=10) had the highest prevalence rate of 11.5%, while the lowest prevalence (1%) was found in individuals of ages 41-60 years. There was no intestinal parasites found among the individuals below11 years of age.. Of the 150 male candidates examined, 57 (65.5%) were infected with intestinal parasites. On the other hand, their female counterparts had a total prevalence of 34.5%. A total number of six parasitic species were isolated from the fecal specimens. These were Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercolaris, Entamoeba coli, Hookworms and Entamoeba histolytica. Ascaris lumbricoides had the highest prevalence rate (12%), while Trichuris trichiura and Strongyloides stercolaris had the least prevalence rate (1%) respectively. The intestinal parasite infection and age were negatively correlated and there was a significant difference between the male and female infection rates (p<0.05).
Occurrence of E. coli O157 H7 from meat products sold in Obinze abattoir, IMO State, Nigeria Osazee Ekundayo Izevbuwa; Shadrach Osalumhense Okhuebor
International Journal of Applied Biology Vol. 4 No. (2) (2020): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

AbstractThis survey was carried out to investigate the occurrence of Escherichia coli O157:H7 in meat products from Obinze abattoir, Imo state, South-Eastern Nigeria. A total of 10 beef samples, 10 processing water samples, 10 table swabs and 20 entrails samples were collected. Pour plate technique was used after a tenfold serial dilution to inoculate on Eosin Methylene Blue (EMB) agar and the E. coli isolates were cultured on Sorbitol MacConkey agar and incubated for 24 hours at 37oC. The isolates were subjected to biochemical tests for identification before antibiotic sensitivity test was carried out using the disc diffusion (Kirby-Bauer) method. From the survey, the entrails had the highest rate of isolation (4.16 ±0.56 log10 Cfu/ml) followed by the beef samples with (3.58 ± 0.01 log10 Cfu/ml). The processing water and the table swabs yielded no growth of E. coli O157:H7. The percentage occurrence of E. coli O157:H7 was also determined and the entrails samples had the highest with (17, 89.5%) followed by the beef samples with (2, 10.5%). The processing water and table swabs samples did not yield growth of E. coli O157:H7 and their percentages were 0. The data obtained were subjected to statistical analysis using the single sample T test which showed that there was no significant difference (p > 0.05) in the rate of isolation of E. coli O157:H7 from the samples. The E. coli O157:H7 isolated showed 100% sensitivity to all the antibiotics used. The presence of E. coli O157:H7 implies that these food samples from the Obinze abattoir, if consumed could be a potential public health hazard to the community. Therefore, strict adherence to quality control measures should be implored in order to reduce contamination and food borne illnesses.Keywords: Escherichia coli O157:H7, public health, hazard, contamination, occurrence
The Seroprevalence of transfusion transmissible hepatitis viruses in Kwara state Nigeria. Osazee Ekundayo Izevbuwa
International Journal of Applied Biology Vol. 6 No. 2 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i2.18622

Abstract

The burden of hepatocellular carcinoma as well as other health complications associated with infections due to Hepatis B and C Viruses are significant around the world. A number of research suggests that assumed healthy living individuals and potential blood donors are the major reservoirs of the Viruses in the community. During the course of this study, a total of 300 apparently healthy blood donors who presented to the blood group serology unit for blood donation across Ilorin, Kwara State, Nigeria, were screened to determine the Sero-prevalence of Transfusion Transmissible Hepatitis Viruses (Hepatitis B and C Viruses). Blood samples obtained from consented potential blood donors were collected and tested for Hepatitis B surface antigen (HBsAg) and Anti-HCV using immunochromatographic methods. The positive samples after the initial qualitative screening were subjected to Enzyme Linked Immunosorbent assay (ELISA) for confirmation of HBV and HCV Immunoglobulin G (IgG) following standard best practices. The results showed that 10% and 0.7% of the blood samples tested positive for HBV and HCV respectively. The male donors had more HBV infection 24 (8.0%) than the females 6 (2.0%). Donors with O Rhesus D positive blood type had more HBV infection 12 (4.0), while blood groups AB Negative, B Negative and O negative showed the least prevalence of 0 (0.0%). The age group of donors above 30 years of age had more HBV prevalence with 37.3% while the age group of 26 to 30 years showed the least prevalence of 28.7%. Data obtained from the piloted structured questionnaires designed to study the socio-demography of the participants showed that HBV was more prevalent among single blood donors, Civil servant/professional workers, and among blood donors that have successfully completed their tertiary education. The data from this research however, showed a negative correlation between the spread of the transfusion transmissible hepatitis viruses and the educational/ occupational standards of the test subjects who were included in this research. The highest rates of viremia were recorded among blood donors residing in urban areas however, the prevalence was not statistically different (p≥0.05).