A. Muktiani
Faculty of Animal and Agricultural Sciences, Diponegoro University Tembalang Campus, Semarang 50275, Central Java

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In Vitro Effects of Oligosaccharides on Bacterial Concentration and Fermentation Profiles in Dairy Calf Colon Content Hendraningsih, L.; Sumantri, C.; Ahmadi, J.; Muktiani, A.; Sulistyanto, B.
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.351 KB)

Abstract

Some oligosaccharides have been shown to modify colonic micro flora composition andactivity in many humans and some animal species. Some oligosaccharides tend to elevate beneficialbacteria and the others depress harmful bacteria in gastrointestinal tract.  However, little data areavailable on their fermentation characteristics by the ilea microbial community, and their effects onthe colon micro flora composition. The aim of this study was to evaluate the in vitro effect ofoligosaccharides from apple and banana peel extraction and mannan-oligosaccharide (MOS)commercial on concentrations of the total anaerobic bacteria, Bifidobacterium, Lactobacilli, Coliformad Esheihia oli populatio i dai alf’ olo otets. To goups of 2 ales fed a ilkreplacer (1 week old) and fibrous pellets (3 weeks-old) were sacrificed 5 h after the morning meal. Ineach group, colon content were pooled and diluted with, diluted by Lowe medium. Extraction ofapple, banana peel, and MOS were tested in vitro versus a control. Each bottle was incubated induplicate for each treatment and at 370C in a shaking water bath. Bacterial concentrations weredetermined at 48 H and inoculated in selected media for each bacteria group and supernatant weretaken for fatty acids (VFA) measurement. Result showed in general, oligosaccharides were notresulted VFA concentration than control bottle in both groups even tend to inclined, specificallybanana peel. Apple peel and banana peel slightly increase total anaerob and lactobacilli in bothgroup but could not depress the E. coli. On the other hand MOS seems the most effective indepressing coli. Based on our results, oligosaccharides were fermented by bacteria of ilea contentsin dairy calves and its fermentation led to a selective stimulation of host bacteria.Key Words: Oligosaccharides, Dairy Calf, Colon, Selective Media
A STUDY ON THE USE OF FECAL CHARACTERISTICS FOR FEED DIGESTIBILITY DETERMINATION IN GOAT Santoso, S.A.B.; Puspitasari, G.; Muktiani, A.; Sunarso, S.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.1.59-67

Abstract

This study aimed to explore physical characteristics and its correlation to feed digestibility in goat.Sixteen Ettawah Grade goats in the second milk production periods and in the 4-5th month of lactationwere used in this study. The study was arranged with completely randomized block block design basedon milk production as replications. The goats were fed ration containing 16% of crude protein and 65%of total digestible nutrients (TDN). Some of crude protein content were supplied by urea. The treatmentswere control ration (without urea; T0), ration with 0.4% urea (T1), ration with 0.8% urea (T2), andration with 1.2% urea (T3). Parameters observed were feed digestibility, fecal extended level (FEL), andfecal rough particle distribution (FRPD). Data were analyzed using correlation-regression to find thecorrelation between fecal characteristics and feed digestibility. The results showed that urea content inthe rations up to 1.2% of dry matter did not affect dry mater digestibility, water content and fecalcharacteristics (FEL and FRPD). There were highly negative correlation (r= -0.665) between feeddigestibility and water content of feces as well as feed digestibility and FEL (r= -0.553), whilecorrelation between digestibility and FRDP was positive (r=0.608). The conclusion of this study waswater content, degree of FEL and FRPD can be used to determine the quality of feed. If feces composedof finer texture, dried and small particles indicated having better digestibility, while if feces composed ofrough texture, wet, and large particles indicated having low digestibility.
Correction to: Supplementation of Zinc Palm Oil Soap Improves Feed Fermentability and Unsaturated Fatty Acid Profile in Rumen Liquid Muktiani, A.; Widiyanto, W.; Pandupuspitasari, N. S.
Tropical Animal Science Journal Vol. 48 No. 3 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.3.279

Abstract

Correction to: Tropical Animal Science Journal, 47(3), 371-380. https://doi.org/10.5398/tasj.2024.47.3.371 An error occurred on Page 373, Table 1, on “Note” under the table. The previous version was written: 1Total digestible nutrients (TDN) were calculated using TDN (%DM). TDN = -17.2649 + 1.2120(PK) + 0.8352(BETN) + 2.4637(LK) + 0.4475(SK), according to Wardeh (1981). The correct version should be: 1Total digestible nutrients (TDN) were calculated using TDN (%DM). TDN = -17.2649 + 1.2120(CP) + 0.8352(NFE) + 2.4637(EE) + 0.4475(CF), according to Wardeh (1981).
Supplementation of Zinc Palm Oil Soap Improves Feed Fermentability and Unsaturated Fatty Acid Profile in Rumen Liquid Muktiani, A.; Widiyanto, W.; Pandupuspitasari, N. S.
Tropical Animal Science Journal Vol. 47 No. 3 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.3.371

Abstract

This study aimed to evaluate the effects of energy and organic zinc supplements, specifically zinc palm oil soap (ZPOS), on digestibility and unsaturated fatty acid profiles in vitro. The study used a completely randomized design with 4 treatments and 5 replications. The treatments were: T0= basal diet without supplementation, T1= basal diet + 5% palm oil (PO), T2= basal diet + 5% partial ZPOS (3.75% ZPOS + 1.25% PO), and T3= basal diet + 5% ZPOS. The inoculum source was rumen liquid from three fistulated female dairy goats and was homogenized. The goats were fed ration consisting of corn straw, soybean hulls, and concentrate containing total digestible nutrients (TDN) 63%, crude protein (CP) 14%, and neutral detergent fiber (NDF) 35%. Results showed that both 5% partial ZPOS and 5% ZPOS supplementation (T2 and T3) resulted in the increase of total volatile fatty acids (VFA), acetate, propionate, butyrate, unsaturated fatty acids (USFA) and a decrease in the ratio of acetate/propionate (A/P) compared to the control and supplementation of 5% PO (p<0.05). Supplementation of 5% partial ZPOS (T2) is better than 5% ZPOS because increased the digestibility of ether extract (EE), crude fiber (CF), NDF, and acids detergent fiber (ADF) (p<0.05) and decreased of methane compared to the control (p<0.05). In conclusion, adding 5% partial ZPOS (3.5% ZPOS and 1.5% PO) increases fiber digestibility, VFA, LCFA, and USFA concentration, and decreases methane production in the rumen liquid.