A. Purnomoadi
Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang 50275

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MICROBIOLOGICAL PROPERTIES OF BEEF IN VARIOUS MEAT SHOPS AT SEMARANG, INDONESIA Nurwantoro, N.; Bintoro, V.P.; Legowo, A.M.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.97-102

Abstract

The aim of the study was to assess microbiological properties of beef sold in various meatshops in Semarang. There are five Indonesian goverment standard requirements to maintain the qualityof beef sold in Indonesia markets, as follows: (1) total plate count (TPC) for a maximum of 106 CFU/g,(2) total coliform bacteria for a maximum of 102 CFU/g, (3) total Escherichia coli up to 10 CFU/g, (4)total Staphylococcus aureus for a maximum of 102 CFU/g and (5) negative for Salmonella per 25 gsamples (SNI 3932, 2008). Beef samples were randomly taken from several traditional markets, meatshops and supermarkets. The result showed that all samples did not contain Salmonella but still couldnot meet one or some of the Indonesian government standard regulation. In conclusion, beef samplesgathered from some of the markets, generaly could not meet one or some of the five strictlyrequirements of the bacteriological properties.
EFFECT OF LEVEL OF CONCENTRATE FEEDING LEVEL ON EFFICIENCY OF EATING BEHAVIOUR ON ONGOLE CROSSBRED CATTLE Dartosukarno, S.; Iskandar, F.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.22-26

Abstract

Eight bulls of Ongole Crossbred (OC) cattle with initial body weight (BW) of 297 + 26 kg (CV =8.75%) fed rice straw treated with urine (RU) (ad libitum) were divided into two groups (each fourheads) to determine the effect of concentrate feeding level on efficiency of eating behavior. The cattlewas given concentrate feeding composed of beer cake and rice bran to make 14% crude protein at 1%and at 2% BW for RUC1 and RUC2 group, respectively. Eating behavior was measured for 3x24 hoursand was performed twice at weeks 2 and 6 of the study. Data obtained were analyzed by t-test. Theresults showed that the level of concentrate feeding affected the intake of urinated rice straw (P<0.01)and daily BWGain (P<0.05), but the effect was not found (P>0.05) on DMI, length time for eating(196.5 vs. 221.5 min/d), length time for rumination (351.0 vs. 449.4 min/d), efficiency of eating time(37.21 vs. 37.67 gDM/min) and efficiency rumination time (21.43 vs. 18.50 gDM/min). This researchshowed that concentrate feeding at 2% BW did not alter the efficiency of eating time and ruminationcompared to 1% BW, although able to improve BWG of OC cattle.
MICROBIOLOGICAL AND PHYSICAL PROPERTIES OF BEEF MARINATED WITH GARLIC JUICE Nurwantoro, N.; Bintoro, V.P.; Legowo, A.M.; Ambara, L.D.; Prakoso, A.; Mulyani, S.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.166-170

Abstract

The study was carried out to investigate the effect of marination, performed by soaking of beef ingarlic juice, on microbiological and physical properties. The study was committed to a completelyrandomized design, with 5 treatments, i.e.: T0 (unmarinated beef, as a control), T1, T2, T3, and T4 thatbeef were marinated in garlic juice for 5, 10, 15, and 20 minutes, respectively, at room temperature(25⁰C). Each treatment consisted of 4 replications. Examination upon experimental parameters wasconducted after marinated (and control) beef was stored for 8 hours at room temperature. Total bacteria,total coliform and water holding capacity of beef were significantly (P<0.05) affected by marinationwith garlic juice. Conversely, cooking loss was not significantly affected (P>0.05) by the treatments. Asa conclusion, marination of beef with garlic juice could reduce total bacteria, total coliform, and waterholding capacity, but could not reduce cooking loss.
THE EFFECT OF ENERGY LEVEL OF FEEDING ON DAILY GAIN, BLOOD GLUCOSE AND UREA ON MADURA CATTLE Umar, M.; Kurnadi, B.; Rianto, E.; Pangestu, E.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.159-166

Abstract

This study was aimed to evaluate the effect of dietary energy level on daily gain of Madura cattle and their blood metabolites (glucose and blood urea). Twelve (12) male Madura cattle aged at 2 years old, 156.27+6.92 kg (CV 4.43%) of body weight were used in this study which was assigned to completely randomized design for three feeding treatments (low, middle and high energy contents) and four replications. The daily gain was measured for 90 days, while the blood metabolites were measured at the middle of experiment. Results showed that dietary energy levels did not affect (P>0.05) average daily gain, dry matter intake, dry matter digestibility, energy intake and feed conversion ratio. Blood glucose and blood urea concentration were ranged at 67-75 and 35-50 mg/dL, respectively, while feed energy efficiency reached 0.145 MJ/g ADG. In conclusion, high-level energy could not provide better performance in Madura cattle because of a limitation factor on the DMI.
ORGANIC ACIDS PRODUCTION OF RICE STRAW FERMENTED WITH SEVERAL TYPES OF MICROORGANISM AT DIFFERENT TEMPERATURES Yanti, Y.; Surahmanto, S.; Purnomoadi, A.; Kawamoto, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.189-194

Abstract

The experiment was carried out to examine the organic acids production of rice straw fermentedwith some types of microorganisms at different temperatures. The experiment was designed as SplitPlot-Completely Randomized Design. The main plot was temperatures treatments (25, 35, 45°C) and thesub plot were microorganisms (Control, Control+Mollases, Lactobacillus fermentum, Bacillus subtilis,Bacillus coagulant, Saccharomyces cerevisiae, Aspergillus niger). The highest lactic acid productionswas in B. coagulans treatment at 35°C (53.79 g/kg DM). The highest acetic acid productions was in L.fermentum at 35°C (13.20 g/kg DM), while the highest propionic acid productions were in Controltreatment at 35°C (0.37 g/kg DM).
A STUDY ON THE USE OF FECAL CHARACTERISTICS FOR FEED DIGESTIBILITY DETERMINATION IN GOAT Santoso, S.A.B.; Puspitasari, G.; Muktiani, A.; Sunarso, S.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.1.59-67

Abstract

This study aimed to explore physical characteristics and its correlation to feed digestibility in goat.Sixteen Ettawah Grade goats in the second milk production periods and in the 4-5th month of lactationwere used in this study. The study was arranged with completely randomized block block design basedon milk production as replications. The goats were fed ration containing 16% of crude protein and 65%of total digestible nutrients (TDN). Some of crude protein content were supplied by urea. The treatmentswere control ration (without urea; T0), ration with 0.4% urea (T1), ration with 0.8% urea (T2), andration with 1.2% urea (T3). Parameters observed were feed digestibility, fecal extended level (FEL), andfecal rough particle distribution (FRPD). Data were analyzed using correlation-regression to find thecorrelation between fecal characteristics and feed digestibility. The results showed that urea content inthe rations up to 1.2% of dry matter did not affect dry mater digestibility, water content and fecalcharacteristics (FEL and FRPD). There were highly negative correlation (r= -0.665) between feeddigestibility and water content of feces as well as feed digestibility and FEL (r= -0.553), whilecorrelation between digestibility and FRDP was positive (r=0.608). The conclusion of this study waswater content, degree of FEL and FRPD can be used to determine the quality of feed. If feces composedof finer texture, dried and small particles indicated having better digestibility, while if feces composed ofrough texture, wet, and large particles indicated having low digestibility.
RUMINAL CONDITION BETWEEN MADURA CATTLE AND ONGOLE CROSSBRED CATTLE RAISED UNDER INTENSIVE FEEDING Umar, M.; Arifin, M.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.213-218

Abstract

Each four young bulls of Madura cattle and Ongole Crossbred (OC) cattle were used to study theefficiency of ruminal fermentation by comparing the proportion of Volatile Fatty Acid (VFA) of thesetwo breeds which were raised under intensive feeding. All the cattle were in about 1.5 years-old with anaverage body weight of 147.75 ± 14.57 kg and 167 ± 22.57 kg, for Madura and OC cattle, respectively.They were fed Napier grass (Pennisetum purpureum) hay, and concentrate feeding consists of pollard,soybean meal and rice bran for 10 weeks. Parameters measured were concentration of VFA at 0, 3 and 6h post-feeding and pH. The concentration of VFA in both Madura and OC cattle was peaked at 3 h postfeeding,being 136.1 mmol and 158.9 mmol, respectively, and then were decreased at 6 h post-feeding ata level of 58.1 and 98.2 mmol, respectively. The proportion of acetic acid in Madura and OC cattle were53.33% and 52.0% of total VFA, respectively, while the proportion of propionic acid and butyric acidwere 28.80% and 17.87% for Madura cattle, and 30.71% and 17.28% for OC cattle, respectively. Inaddition, the Acetic/Propionic ratios were 1.85 and 1.69 for Madura and OC cattle, respectively. RumenpH conditions of both cattle breeds tended to be basic, i.e. Madura cattle was ranged at 8.0-8.4, while thePO cattle was ranged at 7.6-8.4. In conclusion, both cattle breeds (Madura and OC cattle) have a similarefficiency to utilize the feeds in the rumen.
DIETARY ENERGY UTILIZATION IN RAMS BEING FED DURING THE DAY AND/OR AT NIGHT Aprilliza, M.N.; Purnomoadi, A.; Rianto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 2 (2014)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.2.104-110

Abstract

A study was carried out to assess dietary energy utilization in male fat-tailed sheep being fed duringthe day and/or at night. This study were used 12 rams of 12-18 months old with average of initial bodyweight of 27.05±2.9 kg (CV = 10.74%). The experimental design used in this study was a completelyrandomized design (CRD) with 3 treatments and 4 replications. The treatments were feeding during theday (06.00-18.00; DF), feeding at night (18.00-06.00; NF), and feeding all day and night (06.00-06.00;DNF). The diet was offered ad libitum. The results showed that feeding during the day and/or at nightdid not significantly affect (P>0.05) all parameters observed. The average of dry matter intake (DMI),average daily gain (ADG), gross energy intake (GEI), energy digestibility (DE) and energymetabolizability (ME) were 1006 g/d, 75 g/d, 19.2 MJ/d, 74.0% of gross energy, and 66.2% of grossenergy, respectively. The feed efficiency ratio (FCR), gross energy efficiency, digestible energyefficiency, and metabolizable energy efficiency were 7.38%, 3.86 g ADG/MJ, 5.24 g ADG/MJ, and 5.84g ADG/MJ, respectively. It is concluded that time of feeding did not affect the dietary energy utilizationin local rams.
Indirect evaluation of digestive tract function on male lambs and ram based on feed digestibility and eating behavior Prima, A.; Rianto, E.; Purbowati, E.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.124-130

Abstract

This studywasaimed to examine the development and function ofdigestive organsof lamb in digesting feed based on feed digestibility valueandeating behaviour. The materials used were 11 lambs aged 3-4 months, body weight(BW)14.78 ± 1.99 kg (CV 3.98%), and 11 ramsaged 10-12 months, BW 22.18 ± 1.81 kg (CV 3.29%). The study used a completelyrandomized design of 2x2 in nestedpatterns. There were two ages compared (lambs vs ram) and every age was nested the diet i.e.100%Napiergrass (G100) vs mixed pellet 50% grass and 50% concentrate(G50C50). The diet was given at 3.5% of BW. Parameters observed were dry matter intake(DMI)and digestibility, eating and ruminating time. The results showed that were no significant differences between lambs and rams in DMI (averaged 3.33% BW),digestibility (averaged 49.00%), eating (192.56 min/d), and ruminating (364 min/d). Animalsgiven diet G50C50 had higher (P<0.05) DMI (666 vs 565 g/d), digestibility (50.49%vs 47.53%), and less (P<0.05) eating (47 vs 338 min/d) and ruminating (305 vs 423 min/d), as compared withthose fed diet G100.It is concludedthat the digestive tracts of lambs had developed and function to the stage similar to ram. 
THE PERFORMANCE OF JAVA AND ONGOLE CROSSBRED BULL UNDER INTENSIVE FEEDING MANAGEMENT Lestari, C.M.S.; Adiwinarti, R.; Arifin, M.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.109-113

Abstract

This study was set up to evaluate the performance of Java and Ongole Crossbred (OC) bulls fedconcentrate and rice straw. A total of four Java bulls and four OC bulls were used in this experiment. Thebulls were fed concentrates (50% of the total dry matter feed requirement) and rice straw (ad libitum).The concentrates were consisted of rice bran, beer waste product, copra meal, minerals, with crudeprotein (CP) and total digestible nutrients (TDN) contents of 15.32% and 73.09%, respectively. Theaverage daily gain (ADG), dry matter intake (DMI), protein and energy intake, and feed conversion ratio(FCR) were observed. The results of this study showed that the ADG, DMI, CP and TDN intake, andFCR were not significantly different (p> 0.05). The ADG of Java and OC bulls were 0.58 kg and 0.78kg, respectively. The averages of DMI, CP and TDN intake were 6.59 kg (2.09% of BW), 0.81 kg and4.34 kg for Java bulls whereas for OC bulls were 6.42 kg (2.11% of BW), 0.78 kg, and 4.20 kg,respectively. The FCR of Java bulls was 11.49 and those of OC bulls was 9.21. It can be concluded thatJava and OC bulls raised intensively and fed concentrate and rice straw had the similar performance.