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All Journal Jurnal Kesehatan Andalas Mutiara Medika: Jurnal Kedokteran dan Kesehatan Molecular and Cellular Biomedical Sciences (MCBS) International Journal of Cell and Biomedical Science Biosaintifika: Journal of Biology & Biology Education
Sri Priyantini Mulyani
Bagian Ilmu Kesehatan Anak FK Unissula Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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Improving Cytokines and HDL in Diabetic Wistar Rats by Using Combination of Curcumin and Metformin Dinanti, Fathia Kesuma; Mulyani, Sri Priyantini; Chodidjah, Chodidjah
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 23, No 1 (2023): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v23i1.17031

Abstract

In type 2 diabetes mellitus, lipid profile is often found in the form of high-density lipoprotein (HDL) decrease. TNFa and IL-6 are proinflammatory cytokines that are elevated in the blood in many patients with diabetes mellitus. Turmeric is one of the natural ingredients that have anti-cholesterol and anti-inflammatory effects through the work of its active substance, curcumin. This study aims to evaluate the effect of turmeric extract combined with metformin on HDL, TNFa, and IL-6 levels in diabetic rats. Twenty-four Wistar rats were injected intraperitoneally with streptozotocin and niacinamide. The diabetic rats were divided into control, metformin, turmeric, and combination groups. The dose of metformin and turmeric were given, respectively, 45mg/KgBW/day and 200 mg/KgBW/day. At the same time, the combo group was given a half dose of each. Blood serum was taken to examine the HDL, IL-6, and TNFa levels. The levels of HDL, IL-6, and TNFain diabetic rats that received the combination of metformin and turmeric extract (p0.05) were the best among all the groups. It indicated that the combination of metformin and turmeric was better in increasing HDL and reducing IL-6 and TNFa levels better than metformin or turmeric extract alone.
Secretome of Hypoxic Mesenchymal Stem Cells Improves Fluconazole-Induced Alopecia in Rats via Immunoregulatory Modulation of IL-15 and IFN-γ Rahardja, Carolina Kiwik; Mulyani, Sri Priyantini; Putra, Agung
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3500

Abstract

Increased IL-15 and IFN-γ characterize fluconazole-related alopecia (FRA). The hypoxia mesenchymal stem cell (hMSC) secretome has anti-inflammatory capabilities that can potentially be used as alopecia therapy. Therefore, the study aims to determine the effect of topical administration of hMSCs secretome gel on reducing IL-15 and IFN-γ gene expression and baldness in male Wistar rats, a model of FRA. MSC was collected from rat umbilical cord, cultured under hypoxia for 24 hours, and yielded a sterile secretome formulated into a water-based gel ointment for treatment. A total of 24 male Wistar rats were randomly divided into four groups: K1 for health controls with placebo administration only; K2 for negative control which contain FRA rats by applying fluconazole from day 7th to 14th and continued placebo administration until day 29th; treatments were conducted for FRA rat groups treated with 200 mg/day of topical gel contained with 10% of hMSCs secretome for K3 and 20% of hMSCs secretome for K4. Observations were made to analyze hair growth and IL-15 and INF-γ mRNA expression using qPCR. The analysis showed a significant decrease in IL-15 and IFN-γ mRNA expression (p ≤ 0.001) and a reduction in baldness of up to 60% after topical hMSCs secretome gel administration. The prominent result was that the topical gel contained 20% of hMSCs secretome. Based on research results, a topical gel dose containing 20% hMSC secretome had the best effect on improving the condition of FRA. This research may help optimize doses and treatment methods in hMSC secretome therapy.
Therapeutic Potential of Secretome Hypoxia Mesenchymal Stem Cells: Downregulation of TNF-α and HIF-2α in Metabolic Syndrome-Induced Inflammation in Wistar Rats Dewi, Alisia Martha; Trisnasi, Setyo; Putra, Agung; Chodijah , Chodijah; Sarosa, Hadi; Mulyani, Sri Priyantini; Amalina, Nur Dina; Ibrahim, Sugeng
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6613

Abstract

Background: Metabolic syndrome (MetS) has become a global health challenge with several associated issues, such as obesity, insulin resistance, dyslipidemia, and hypertension. Important proteins such as Tumor Necrosis Factor-alpha (TNF-α) and Hypoxia Inducible Factor-2 alpha (HIF-2α) regulate the inflammatory process by inducing the expression of pro-inflammatory proteins. Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) are immunomodulatory, anti-inflammatory, and angiogenic stimulators, which can regulate various inflammatory diseases, including MetS. Objective: This study aims to determine the effect of administering SH-MSCSs on the expression of the TNF-α and Hypoxia Inducible Factor (HIF)-2α genes in the male Wistar rat model with Metabolic Syndrome. Method: This research is an experimental study with a Post-test Only Control Group Design, using a total of 24 male Wistar rats divided into four groups: T1 (Healthy control), T2 (MetS + NaCl), T3 (MetS + administration of SH-MSCs dose 150 uL), and T4 (MetS + administration of SH-MSCs dose 300 uL). SH-MSCSs were administered intraperitoneally four times over 14 days. Adipose tissue TNF-α and HIF-2α gene expression were measured on day 15 using qRT-PCR. Results: TNF-α and HIF-2α gene expression was significantly lower in T3 and T4, compared with the MetS control group (T2). Conclusion: Administration of SH-MSCs was able to reduce the expression of the Tumor Necrosis Factor (TNF-α) and Hypoxia Inducible Factor (HIF)-2α genes in fatty tissue in the male Wistar rat model with Metabolic Syndrome.
The Effect of Hypoxic Mesenchymal Stem Cells on the expression of Transforming Growth Factors in Wistar Rats Excision Wound Model Istiqomah, Dyah Ayu Fitri; Djannah, Durrotul; Mulyani, Sri Priyantini
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.16

Abstract

Background: Hypoxic Mesenchymal stem cell (MSC) therapy may accelerate the wound healing process through a paracrine mechanism by increasing the expression of transforming growth factor-β (TGF-β). Objective: This study aims to investigate the effect of hypoxic MSC on TGF-β gene expression in excision wound models. Methods: This is an experimental study with a post-test-only control group design. Sixty male Wistar rats were divided into 4 groups consisting of 5 each to represent group I (sham/normal control), group II (excision wound model), group III (excision wound model and normoxic MSC injection), group IV (excision wound model and hypoxic MSC injection) for observation of TGF-β gene expression on days 3, 6 and 9. Both MSC (3x106 cells) were injected subcutaneously after wound excision at five locations 0,5 cm from the wound edge. TGF-β gene expression was examined by qRT-PCR. Results: The highest average TGF-β gene expression on the three observation days were shown by group II. TGF-β gene expression in groups III and IV was lower than in group II, while groups III and IV were relatively similar. In the normal wound healing process, TGF-β is highly expressed, and both MSC injection reduces TGF-β gene expression. Conclusion: Hypoxic MSC injection accelerated the proliferative phase of the excisional wound healing process, but the acceleration effect was equivalent to that shown by Normoxic MSC.
Pengaruh Kombinasi Metformin dan Ekstrak Kunyit terhadap Kadar High Density Lipoprotein, Interleukin-6 dan Tumor Necrosis Factor-a Dinanti, Fathia Kesuma; Mulyani, Sri Priyantini; -, Chodidjah -
Jurnal Kesehatan Andalas Vol. 11 No. 3 (2022): Online November 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i3.2074

Abstract

A  decrease in high-density lipoprotein (HDL) happens in type 2 diabetes mellitus. The cytokines TNFa and IL-6 are proinflammatory cytokines that are elevated in the blood in many patients with DM. Turmeric is one of the natural ingredients with antioxidant, anti-cholesterol, and anti-inflammatory properties through its active substance, curcumin. Objective: To prove the effectiveness of the combination of metformin and turmeric extract on HDL levels, TNFa levels, and IL-6 levels in Streptozotocin-Nicotinamide-induced Wistar rats. Methods: The study used a post-test-only controlled group design. The subjects were 24 male Wistar rats which were randomly divided into four groups, namely K1, K2, P1, and P2. The whole group was a type 2 DM model induced by streptozotocin-nicotinamide. Group K2 was given metformin 45mg/KgBW/day, group P1 was given turmeric extract at a dose of 200 mg/KgBW/day, and group P2 was given a combination of metformin and turmeric extract. On day 15, blood was drawn for HDL, IL-6, and TNFa examination. The data were analyzed using the one-way Anova test. Results: The mean HDL level in the P2 group (72,4±33,31) was the highest compared to the other three groups. The lowest mean TNFa level among the four groups was the P2 group (7,56±0,44). The lowest mean levels of IL-6 were obtained in the P2 group (58,82±2,15). One-way Anova test on HDL, IL-6, and TNFa levels showed a significant difference between groups with p-value = 0.000. Conclusion: The combination of metformin and turmeric extract can increase HDL levels and reduce IL-6 levels and TNFa levels better than giving metformin or turmeric extract alone.Keywords:  turmeric extract, HDL, IL-6, TNFa
Exosome Therapy from Hypoxia-treated Mesenchymal Stem Cells Reduces TNF-α and Increases VEGF Levels in Fluconazole-Induced Alopecia Model Sulistami, Siska Marlina; Mulyani, Sri Priyantini; Putra, Agung; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 9, No 3 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i3.642

Abstract

Background: Alopecia is a condition with partial or complete hair loss, leading to psychological distress. Current treatments, such as minoxidil and finasteride, have limited efficacyand side effects. Recent studies suggest that mesenchymal stem cells (MSCs)-derived exosomes offer regenerative potential by modulating inflammation and enhancing hair follicle regeneration, though optimal dosage remains unclear. Tumor necrosis factor-alpha (TNF-α)  inhibits hair follicle growth, while vascular endothelial growth factor (VEGF) promotes hair regrowth. This study evaluates  exosome therapy from hypoxia (Hypo-Exo)-treated MSCs in modulating TNF-α and VEGF in a fluconazole-induced alopecia-like model..Materials and methods: An experimental post-test only control group design was used with 30 male Wistar rats, divided into five groups:  Healthy group, 0.9% NaCl-treated group, 5% Minoxidil-treated group, 100 μg/mL Hypo-Exo MSCs-treated group, and 200 μg/mL Hypo-Exo MSCs-treated group. TNF-α and VEGF levels were analyzed using ELISA on day 14 post-treatment. Results: The highest TNF-α level was found in the 0.9% NaCl-treated group (307.46 ± 20.68 pg/mL) and significantly reduced (p<0.05) in 100 μg/mL Hypo-Exo MSCs-treated group (65.38±15.05 pg/mL) and 200 μg/mL Hypo-Exo MSCs-treated group (37.16±7.14 pg/mL). VEGF levels were the highest in the 200 μg/mL Hypo-Exo MSCs-treated group (189.11±9.75 pg/mL) and 100 μg/mL Hypo-Exo MSCs-treated group (158.50±5.33 pg/mL), compared to the 0.9% NaCl-treated group (69.60±15.39 pg/mL). Conclusion: Hypo-Exo MSCs significantly reduced TNF-α and increased VEGF levels, supporting their potential as a novel regenerative therapy for alopecia. Keywords: alopecia, TNF-α, VEGF, exosome, hypoxia, mesenchymal stem cells 
Co-Authors Agung Putra Amalina, Nur Dina Chodidjah Chodidjah Chodijah , Chodijah Dewi, Alisia Martha Dinanti, Fathia Kesuma Djannah, Durrotul Eko Setiawan Hadi Sarosa Ibrahim, Sugeng Istiqomah, Dyah Ayu Fitri Rahardja, Carolina Kiwik Sulistami, Siska Marlina Trisnasi, Setyo