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All Journal Jurnal Kesehatan Andalas MEDISAINS Mutiara Medika: Jurnal Kedokteran dan Kesehatan Molecular and Cellular Biomedical Sciences (MCBS) International Journal of Cell and Biomedical Science
Sri Priyantini Mulyani
Bagian Ilmu Kesehatan Anak FK Unissula Semarang
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Improving Cytokines and HDL in Diabetic Wistar Rats by Using Combination of Curcumin and Metformin Dinanti, Fathia Kesuma; Mulyani, Sri Priyantini; Chodidjah, Chodidjah
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 23, No 1 (2023): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v23i1.17031

Abstract

In type 2 diabetes mellitus, lipid profile is often found in the form of high-density lipoprotein (HDL) decrease. TNFa and IL-6 are proinflammatory cytokines that are elevated in the blood in many patients with diabetes mellitus. Turmeric is one of the natural ingredients that have anti-cholesterol and anti-inflammatory effects through the work of its active substance, curcumin. This study aims to evaluate the effect of turmeric extract combined with metformin on HDL, TNFa, and IL-6 levels in diabetic rats. Twenty-four Wistar rats were injected intraperitoneally with streptozotocin and niacinamide. The diabetic rats were divided into control, metformin, turmeric, and combination groups. The dose of metformin and turmeric were given, respectively, 45mg/KgBW/day and 200 mg/KgBW/day. At the same time, the combo group was given a half dose of each. Blood serum was taken to examine the HDL, IL-6, and TNFa levels. The levels of HDL, IL-6, and TNFain diabetic rats that received the combination of metformin and turmeric extract (p0.05) were the best among all the groups. It indicated that the combination of metformin and turmeric was better in increasing HDL and reducing IL-6 and TNFa levels better than metformin or turmeric extract alone.
The Effect of Hypoxic Mesenchymal Stem Cells on the expression of Transforming Growth Factors in Wistar Rats Excision Wound Model Istiqomah, Dyah Ayu Fitri; Djannah, Durrotul; Mulyani, Sri Priyantini
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.16

Abstract

Background: Hypoxic Mesenchymal stem cell (MSC) therapy may accelerate the wound healing process through a paracrine mechanism by increasing the expression of transforming growth factor-β (TGF-β). Objective: This study aims to investigate the effect of hypoxic MSC on TGF-β gene expression in excision wound models. Methods: This is an experimental study with a post-test-only control group design. Sixty male Wistar rats were divided into 4 groups consisting of 5 each to represent group I (sham/normal control), group II (excision wound model), group III (excision wound model and normoxic MSC injection), group IV (excision wound model and hypoxic MSC injection) for observation of TGF-β gene expression on days 3, 6 and 9. Both MSC (3x106 cells) were injected subcutaneously after wound excision at five locations 0,5 cm from the wound edge. TGF-β gene expression was examined by qRT-PCR. Results: The highest average TGF-β gene expression on the three observation days were shown by group II. TGF-β gene expression in groups III and IV was lower than in group II, while groups III and IV were relatively similar. In the normal wound healing process, TGF-β is highly expressed, and both MSC injection reduces TGF-β gene expression. Conclusion: Hypoxic MSC injection accelerated the proliferative phase of the excisional wound healing process, but the acceleration effect was equivalent to that shown by Normoxic MSC.
Pengaruh Kombinasi Metformin dan Ekstrak Kunyit terhadap Kadar High Density Lipoprotein, Interleukin-6 dan Tumor Necrosis Factor-a Dinanti, Fathia Kesuma; Mulyani, Sri Priyantini; -, Chodidjah -
Jurnal Kesehatan Andalas Vol. 11 No. 3 (2022): Online November 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i3.2074

Abstract

A  decrease in high-density lipoprotein (HDL) happens in type 2 diabetes mellitus. The cytokines TNFa and IL-6 are proinflammatory cytokines that are elevated in the blood in many patients with DM. Turmeric is one of the natural ingredients with antioxidant, anti-cholesterol, and anti-inflammatory properties through its active substance, curcumin. Objective: To prove the effectiveness of the combination of metformin and turmeric extract on HDL levels, TNFa levels, and IL-6 levels in Streptozotocin-Nicotinamide-induced Wistar rats. Methods: The study used a post-test-only controlled group design. The subjects were 24 male Wistar rats which were randomly divided into four groups, namely K1, K2, P1, and P2. The whole group was a type 2 DM model induced by streptozotocin-nicotinamide. Group K2 was given metformin 45mg/KgBW/day, group P1 was given turmeric extract at a dose of 200 mg/KgBW/day, and group P2 was given a combination of metformin and turmeric extract. On day 15, blood was drawn for HDL, IL-6, and TNFa examination. The data were analyzed using the one-way Anova test. Results: The mean HDL level in the P2 group (72,4±33,31) was the highest compared to the other three groups. The lowest mean TNFa level among the four groups was the P2 group (7,56±0,44). The lowest mean levels of IL-6 were obtained in the P2 group (58,82±2,15). One-way Anova test on HDL, IL-6, and TNFa levels showed a significant difference between groups with p-value = 0.000. Conclusion: The combination of metformin and turmeric extract can increase HDL levels and reduce IL-6 levels and TNFa levels better than giving metformin or turmeric extract alone.Keywords:  turmeric extract, HDL, IL-6, TNFa
Exosome Therapy from Hypoxia-treated Mesenchymal Stem Cells Reduces TNF-α and Increases VEGF Levels in Fluconazole-Induced Alopecia Model Sulistami, Siska Marlina; Mulyani, Sri Priyantini; Putra, Agung; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 9, No 3 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i3.642

Abstract

Background: Alopecia is a condition with partial or complete hair loss, leading to psychological distress. Current treatments, such as minoxidil and finasteride, have limited efficacyand side effects. Recent studies suggest that mesenchymal stem cells (MSCs)-derived exosomes offer regenerative potential by modulating inflammation and enhancing hair follicle regeneration, though optimal dosage remains unclear. Tumor necrosis factor-alpha (TNF-α)  inhibits hair follicle growth, while vascular endothelial growth factor (VEGF) promotes hair regrowth. This study evaluates  exosome therapy from hypoxia (Hypo-Exo)-treated MSCs in modulating TNF-α and VEGF in a fluconazole-induced alopecia-like model..Materials and methods: An experimental post-test only control group design was used with 30 male Wistar rats, divided into five groups:  Healthy group, 0.9% NaCl-treated group, 5% Minoxidil-treated group, 100 μg/mL Hypo-Exo MSCs-treated group, and 200 μg/mL Hypo-Exo MSCs-treated group. TNF-α and VEGF levels were analyzed using ELISA on day 14 post-treatment. Results: The highest TNF-α level was found in the 0.9% NaCl-treated group (307.46 ± 20.68 pg/mL) and significantly reduced (p<0.05) in 100 μg/mL Hypo-Exo MSCs-treated group (65.38±15.05 pg/mL) and 200 μg/mL Hypo-Exo MSCs-treated group (37.16±7.14 pg/mL). VEGF levels were the highest in the 200 μg/mL Hypo-Exo MSCs-treated group (189.11±9.75 pg/mL) and 100 μg/mL Hypo-Exo MSCs-treated group (158.50±5.33 pg/mL), compared to the 0.9% NaCl-treated group (69.60±15.39 pg/mL). Conclusion: Hypo-Exo MSCs significantly reduced TNF-α and increased VEGF levels, supporting their potential as a novel regenerative therapy for alopecia. Keywords: alopecia, TNF-α, VEGF, exosome, hypoxia, mesenchymal stem cells 
In vivo study on the effect of noni leaf extract cream (Morinda Citrifolia L) on PDGF and TNF-α levels in cut wounds Sari, Shintia Devi Arum; Mulyani, Sri Priyantini; Putra, Agung; Setiawan , Eko
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Vol. 23 No. 3 (2025)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v23i3.25252

Abstract

ABSTRACT Background: Skin wounds damage the structure and epithelial tissue, resulting in scar tissue formation. Prolonged wound healing or excessive body response will inhibit the normal process and produce unaesthetic scar tissue. Many plant extracts and their active components can accelerate the wound healing process, Noni leaf extract has antioxidant activity and accelerates wound healing. Objective: This study aims to determine the effect of noni leaf extract cream (Morinda Citrifolia L) on TNF-α and PDGF levels in Wistar rats with incised wounds. Method: A laboratory experimental study with a post-test only control group design. Consisting of five treatment groups, namely the healthy rat group (KS), Negative group (KN) rats with incised wounds without treatment, positive group (KP) rats with incised wounds that were smeared with povidone-iodine cream, treatment group 1 (P1) rats that were smeared with 20% noni leaf extract cream, and treatment group 2 (P2) rats that were smeared with 40% noni leaf extract cream, treatment for 3 days then examined the levels of TNF-α and PDGF skin tissue using the ELISA method. Results: The average TNF-α levels showed a significant difference between groups p of 0.000 (p <0.05) with the One way anova test, the highest average in group (KN) 220.68 pg / mL, there was a decrease in all treatment groups with the lowest TNF-α levels in group (KP) 76.59 pg / mL. The average results of TNF-α levels showed a significant difference of p 0.000 (<0.05) with the One-way ANOVA test, the group without intervention (KP) had the lowest PDGF levels while the group (P2) experienced the highest increase in PDGF levels. Conclusion: Administration of noni leaf extract cream with doses of 20% and 40% had an effect on reducing TNF-α levels and increasing PDGF levels in Wistar rats with incised wounds.
Co-Authors Agung Putra Chodidjah Chodidjah Dinanti, Fathia Kesuma Djannah, Durrotul Eko Setiawan Istiqomah, Dyah Ayu Fitri Sari, Shintia Devi Arum Setiawan , Eko Sulistami, Siska Marlina