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All Journal Indonesian Journal of Cancer Chemoprevention
Hilyatul Fadliyah
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells Hilyatul Fadliyah; Nindya Budiana Putri; Ziana Walidah; Ika Putri Nurhayati; Muthi Ikawati; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp68-77

Abstract

The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that 5 μg/mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 μg/mL BPEE caused lower expression of both MMPs. The combination of 5 μg/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further.Keywords : Boesenbergia pandurata, doxorubicin, MCF-7/HER2, migrationSubmitted:
Fingerroot (Boesenbergia pandurata) : A Prospective Anticancer Therapy Marsya Yonna Nurrachma; Hilyatul Fadliyah; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp102-109

Abstract

Beside as a spice in Indonesian cooking, Fingerroot (Boesenbergia pandurata) regularly is used as a mixture of herbal medicine. Scientifically, the phytochemical content of the fingerroot rhizome showed some therapeutical effects such as antibacterial, anti-inflammatory, anti or pro-oxidant, and also anticancer. In this article, we summarize some studies especially about anticancer activity of fingerroot and its constituent coumpound. We found that fingerroot is capable of inhibiting various pathways of cell physiology processes. One potential pathway to be inhibited by fingerroot is Poly (ADP-ribose) polymerase (PARP) which has role in apoptotic induction. In the future, it is necessary to purify the extract to obtain maximum efficacy and also formulation studies of fingerroot will be interesting to do to.Keywords : fingerroot, anti-cancer, chemopreventive, herbal medicineBeside as a spice in Indonesian cooking, Fingerroot (Boesenbergia pandurata)regularly is used as a mixture of herbal medicine. Scientifically, the phytochemical contentof the fingerroot rhizome showed some therapeutical effects such as antibacterial, antiinflammatory,anti or pro-oxidant, and also anticancer. In this article, we summarizesome studies especially about anticancer activity of fingerroot and its constituentcoumpound. We found that fingerroot is capable of inhibiting various pathways of cellphysiology processes. One potential pathway to be inhibited by fingerroot is Poly (ADPribose)polymerase (PARP) which has role in apoptotic induction. In the future, it isnecessary to purify the extract to obtain maximum efficacy and also formulation studiesof fingerroot will be interesting to do to.
Red Betel Leaves Methanolic Extract (Piper crocatum Ruiz & Pav.) Increases Cytotoxic Effect of Doxorubicin on WiDr Colon Cancer Cells through Apoptosis Induction Nindi Wulandari; Argandita Meiftasari; Hilyatul Fadliyah; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 9, No 1 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss1pp1-8

Abstract

Doxorubicin is a chemotherapeutic agent that causes a lot of side effects in high doses. Thus, combination with a co-chemotherapeutic agent which can increase its toxicity on cancer cells, is needed to reduce its therapeutic dose. Red betel leaves (Piper crocatum Ruiz & Pav.) have been known to contain flavonoids and alkaloids that have anticancer activity. This study was conducted to determine the cytotoxic effect and apoptosis induction of red betel leaves methanolic extract (RBM), doxorubicin (dox) and the combination of them on WiDr cells as model of colon cancer. RBM was extracted by soxhlet method using methanol. Cytotoxicity assay was performed using MTT assay for both single and combination treatments for 24 hours to determine IC50 and CI as their parameters. Apoptosis induction was analyzed by double staining method using ethidium bromide-acridine orange staining. Treatment of RBM and dox on WiDr cells for 24 hours showed cytotoxic activity with IC50 100 μg/mL and 1.6 μM respectively. Combination of RBM and dox performed synergism effect with CI<0.9 (p<0.05). Combination of RBM (12.5 μg/mL) and dox (0.4 μM) increased the number of apoptosis cells compared to each single treatment. Based on this study, it can be concluded that red betel leaves methanolic extract is potential to be developed as a co-chemotherapeutic agent of doxorubicin on colon cancer but still need further study to disclose the underlying molecular mechanisms.Keywords :  Red betel leaves (Piper crocatum Ruiz & Pav.), doxorubicin, WiDr cells, co- chemotherapeutic agent
Antigenotoxic Activity of Rumput Mutiara (Hedyotis corymbosa L.) Ethanolic Extract on Cyclophosphamide-Induced Mice Yoce Aprianto; Asri Mega Putri; Hilyatul Fadliyah; Retno Murwanti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp135-145

Abstract

Exposure to relative chemicals has been shown to induce a genotoxic effect that can be observed through formation of micronucleus (MN) in polychromatic erythrocythes (PCE). Rumput Mutiara or Hedyotis corymbosa L. ethanolic extract (HcEE) is known to contain ursolic acid as major compound that possesses antigenotoxic activity on HepG2 cells. This study exerts in vivo approach aiming to evaluate the antigenotoxic effects of HcEE on cyclophosphamide (CP)-induced male Swiss mice. The ursolic acid on HcEE was determined by using thin layer chromatography with silica gel as stationary phase and chloroform-aceton (9:1) as mobile phase. The antigenotoxic activity was carried out by in vivo micronucleus test. Twenty four adult mice were equally divided into seven groups. Group I: control (untreated); group II: Na-CMC 0.5%; group III: CP 50 mg/kg BW; group IV: CP+HcEE 250 mg/kg BW; group V: CP+HcEE 500 mg/kg BW; group VI: CP+HcEE 1000 mg/kg BW; group VII: HcEE 1000 mg/kg BW. HcEE were given for seven days, while CP was administered on the last two days. On the seventh day, the peripheral blood from all mice were collected, smeared, and then stained with Giemsa. The frequencies of MNPCEs and %PCEs were evaluated. Molecular docking was performed to know the interaction between ursolic acid and CYP3A4 by using PLANTS software. There was similar hRF spot between HcEE with ursolic acid standard reference indicated that the extract almost positively contain ursolic acid. HcEE reduced MNPCEs significantly compared to CP group (p<0.05) and combination of CP with HcEE showed reduction of %PCEs (p<0.05). Based on molecular docking analysis, ursolic acid gave lower docking score than CP against CYP3A4 (PDB ID: 2V0M) and similar binding site on amino acid residues Ala 448, Ile 369, Thr 309, and Val 313. All of these data suggest that HcEE perform protective effect against CP-induced genotoxicity.Keywords: Antigenotoxic, Hedyotis corymbosa L., cyclophosphamide, micronucleus, molecular docking
Co-Authors Argandita Meiftasari Asri Mega Putri Edy Meiyanto Edy Meiyanto Edy Meiyanto Ika Putri Nurhayati Marsya Yonna Nurrachma Muthi Ikawati Nindi Wulandari Nindya Budiana Putri Retno Murwanti Riris Istighfari Jenie Yoce Aprianto Ziana Walidah