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All Journal Indonesian Journal of Cancer Chemoprevention Universa Medicina
Etty Widayanti
Department of Anatomy, Faculty of Medicine, YARSI University
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Anticancer Effect of a Typhonium flagelliforme L. in Raji Cells Through Telomerase Expression Endang Purwaningsih; Yulia Suciati; Etty Widayanti
Indonesian Journal of Cancer Chemoprevention Vol 8, No 1 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss1pp15-20

Abstract

Cancer cells have a relatively high telomerase activity compared to normal cells, so the cancer cells have the ability to continue to proliferate and undergo mitosis uncontrolled. Telomerase is an enzyme which responsible for telomere length, a DNA segment that is at the end of eukaryotic cell chromosomes. Telomeres and telomerase play a role in the incidence of carcinogenesis. Natural materials such as taro mice (Typhonium flagelliforme) have potential as anticancer. The purpose of the study was to determine the effects of plant extracts of rat taro on the expression of telomerase in cancer cell Raji. The research method is experimental studies in some form cancerous cell culture cell line, Raji. Used as a control normal cells is Vero cell. The Culture medium used RPMI for Raji cell  and M199 for Vero cell. The study consisted of three groups, control, doses of 1 ½ IC50 and IC50 doses. Expression of telomerase enzyme was measured by the Immunohystochemistry method (IHC). The results showed that the expression of telomerase in cancer cells showed values significantly higher than the normal cells (Vero). Giving mice taro plant extracts (Typhonium flagelliforme) were able to decrease the expression of telomerase significantly in both treatment doses. It was concluded that rodent tuber extract (Typhonium flagelliforme Lodd) can reduce the expression of telomerase in Raji cells, so that the rodent tuber extract (Typhonium flagelliforme Lodd) has potential as an anticancer through the expression of telomerase.Keywords: telomerase, IHC, Typhonium flagelliforme
Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells Endang Purwaningsih; Etty Widayanti; Yulia Suciati
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.75-82

Abstract

BACKGROUNDCancer is one of the causes of high mortality. Breast and cervical cancers are two of the most frequent cancers affecting women around the world, including Indonesia. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potentials. The rodent tuber extract contains ribosome inactivating proteins (RIPs) capable of cutting the DNA or RNA of cancer cells and blocking the growth of cancer cells. The purpose of this study was to evaluate the cytotoxic effects of Typhonium flagelliforme Lodd extract on HeLa cervical cancer and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells.METHODSSubjects were cultured cell lines of HeLa cells in Rosswell Park Memorial Institute (RPMI) and of MCF-7 cells in Dulbecco’s Minimum Essential Medium (DMEM). Rodent tuber ethanolic extract was diluted in dimethyl sulfoxide (DMSO). The cytotoxicity assay used the 3-(4,5-dimethyl thiazol-2-yl,5-diphenyl) tetrazolium bromide (MTT) method. Absorbance was read in an ELISA reader at a wavelength of 595 nm.RESULTSRat tuber extract at all dilutions (500; 250; 125; 62.5; 31.25; 15.625;7.81; 3.9 ì g/ mL) showed cytotoxic effects against HeLa and MCF-7 cells. Higherconcentrations of the extract gave a higher proliferation inhibition effect.Calculated IC50 values of the extract by probit analysis were 30.19 ìg/mL against HeLa cells and 5.586 ì g/mL against MCF-7 cells.CONCLUSIONSEthanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells. The cytotoxic effects against MCF-7 cells are greater than the cytotoxic effects against HeLa cells.
Co-Authors Endang Purwaningsih Yulia Suciati