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All Journal Jurnal Ilmu-Ilmu Pertanian Indonesia SINTA Journal (Science, Technology, and Agricultural)
Yenny Sariasih
University of Bengkulu
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Physics Veterinary

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DETEKSI VIRUS TUNGRO PADA PADI DI BENGKULU Mimi Sutrawati; Yenny Sariasih; Priyatiningsih Priyatiningsih; Fausiah T. Ladja
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 21, No 2 (2019)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.21.2.99-102

Abstract

[DETECTION OF TUNGRO VIRUS ON RICE IN BENGKULU]. Several types of viruses have been reported to infect rice plants in Indonesia, including tungro, dwarf grass, and empty dwarfs. The spread of tungro in Indonesia in the beginning was only limited to certain regions in South Sulawesi, South Kalimantan, West Nusa Tenggara and North Sulawesi, but later it expanded to East Java, Central Java and Yogyakarta. In this study a tungro disease survey was conducted in several areas in Bengkulu Province. Virus detection is done by Loop-medated isothermal amplification (LAMP). Observations in the field showed that there were typical symptoms of tungro infection in rice plants, namely orange leaves starting from leaves to 2 and 3, little tillers, stunted plant growth. The results of detection with LampPCR showed rice samples from the district Pondok Kelapa Kab Bengkulu Tengah is positively infected with tungro virus.
Inventory and Identification of Pathogens, Nematodes, and Entomopathogens Associated with Maize (Zea mays L.) in Rejang Lebong Regency, Bengkulu Province Yenny Sariasih; Sempurna Ginting; Djamilah; Mimi Sutrawati; Ria Dwi Putri Anggrea Nengsih; Fiqri Ardiansyah; Putri Anisa
SINTA Journal (Science, Technology, and Agricultural) Vol. 7 No. 1 (2026)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.7.1.1-10

Abstract

Maize (Zea mays L.) is a strategic crop whose productivity is significantly affected by diseases caused by various plant pathogens. This study aimed to inventory and identify pathogens, nematodes, and entomopathogenic fungi associated with maize based on field symptoms, morphological characteristics, and molecular identification, specifically for the fungal pathogens detected. The research was conducted in major maize-producing areas of Rejang Lebong Regency, Bengkulu Province, Indonesia, located at an altitude of 700–900 m above sea level. Samples consisting of leaves, roots, and rhizosphere soil were collected from several maize fields. Field symptoms were observed, followed by pathogen isolation and morphological identification. Entomopathogenic fungi were cultured and purified on Sabouraud Dextrose Agar supplemented with Yeast Extract (SDAY) medium, while soil samples were processed using a modified sieving technique to extract nematodes. The results revealed four major diseases affecting maize, namely downy mildew, common rust, northern leaf blight, and leaf spot, caused by Peronosclerospora maydis, Puccinia sorghi, Exserohilum turcicum, and Cercospora sp., respectively. Six genera of plant-parasitic nematodes were identified, including Meloidogyne sp., Pratylenchus sp., Helicotylenchus sp., Rotylenchus sp., Tylenchus sp., and Nacobbus aberrans, all associated with root damage. Only one species of entomopathogenic fungus, Nomuraea rileyi, was isolated, indicating its potential as a biological control agent against insect pests. This study provides baseline information on the diversity of pests and pathogens associated with maize in Rejang Lebong Regency, which is essential for supporting integrated and sustainable pest and disease management.
Co-Authors Djamilah Fausiah T. Ladja Fiqri Ardiansyah Mimi Sutrawati Mimi Sutrawati Priyatiningsih Priyatiningsih Putri Anisa Ria Dwi Putri Anggrea Nengsih Sempurna Ginting