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Inhibitory response of Pectobacterium carotovorum subsp. carotovorum(Jones) Dye Applied with Betel Leaf and Areca Nut Extract as a Potential Organic Bactericide Emerensiana - Uge; Titik Sri Harini; Sri Widinugraheni; Jilinda B.D Henuk
Cannarium Vol 18, No 1 (2020)
Publisher : Universitas Khairun

Soft root caused by the bacterium Pectobacterium carotovorum subsp carotovorum (Jones) Dye (P.c.c), is a disease that is very detrimental to horticultural plants, especially carrot. The disease control is commonly done by chemical techniques, but because of the negative impact, it is important to choose an environmentally friendly techniques, such as plant extracts. The aim of this research is to know response of P.c.c bacteria growth when it is applied with betel leaves extracts (BTL) and Areca nut extracts (ANE) in different concentrations.This research is begun with pathogenecity test, Gram coloring test, and Growing on selective media. While the inhibation testing was carried out in In Vitro. Experiment were carried out with agarose-wells method, where bacterial inoculum was grown with agarose medium, then plant extract added in to medium through the well. The observation done by measured inhibitory zone diameter (mm) around agarose-wells. The data presented the size of the inhibitory zone diameter (mm) formed on P.c.c culture media, then was analyzed using ANOVA followed by Duncan’s test.Pathogenecity test, Gram-coloring test, and growing selective media test showed that there was symptoms on 4 days after infection, red and rod-shaped bacteria, while in CVD medium, the bacteria grow uniformly at 37 0 C, although they do not form a basin in the medium. And In vitro test showed that the treatment of BTL, and ANE at 10%, 5%, and 10% concentration respectively, had good ability to inhibit bacteria in NA medium, with the average inhibition zone diameters of 32.33 mm, 28.67 mm, and 33 .67 mm.

Keanekaragaman Serangga Hama dan Musuh Alami pada Pertanaman Kacang Hijau (Vigna radiata) di Desa Lakekun Kecamatan Kobalima Kabupaten Malaka Fransiska Manek; Titik Sri Harini; Petronella S. Nenotek
Wana Lestari Vol 5 No 2 (2023): Wana Lestari
Publisher : Program Studi Kehutanan, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/wanalestari.v5i02.13479

This research was conducted in Lakekun Village, Kobalima District, Malaka Regency from May to July 2023, aiming to determine the types of insect pests and natural enemies in mung beans. Data collection was carried out using direct observation techniques on mung bean plants and sampling using several traps. The types of traps used were pitfall trap, swepnet, yellow sticky trap, swing net and light trap. Sampling was done diagonally to determine the sample unit. The number of observation plots in the field is 3 observation plots. in 1 plot there are 5 sample units and a total of 3 observation plots there are 15 aub plots observed. Insect pests found on mung bean in the research location have three roles, namely as pests, predators and parasitoids. Species that act as pests consist of Artherigona exigua, Nezara viridula L, Aphis craccivora, Halyomopha halys, Riptortus linearis, Adoretus sp. and Gralliclava horrens. Insects that act as predators consist of Menochilus sexmaculatus, Coccinella transversalis, Orthhetrum sabina, Iscunara senegalensis. While insects that act as parasitoids are Tiphia femorata. The highest population found at the research site was Aphis craccivora which amounted to 315 individuals and the lowest population was Graliclava horrens with a total of 7 individuals. The value of the diversity index in mung bean plants is classified as moderate, namely 1.89. Keywords: diversity, insects, mung bean, identification.

Keanekaragaman Serangga Hama dan Musuh Alami Pada Tumpangsari Tanaman Jagung dan Kacang Tanah di Desa Oemasi Kecamatan Nekamese Kabupaten Kupang Seviana Jebe; Titik Sri Harini; Rika Ludji; Agustina Etin Nahas
Wana Lestari Vol 6 No 1 (2024): Wana Lestari
Publisher : Program Studi Kehutanan, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/wanalestari.v6i01.16775

Maize and groundnuts are commodities that are always cultivated by farmers, including in East Nusa Tenggara Province. The cropping system of these two commodities also varies, both with monoculture and intercropping patterns. The main obstacle that is often faced in the cultivation of corn and peanut plants is the attack of pests and pathogens that cause disease. This study aims to determine the types of insect pests and natural enemies in intercropping corn and peanut plants, the population of each type of insect and the diversity index of insect pests and natural enemies in intercropping corn and peanuts. This study used a survey method with direct observation techniques on each trap. Sampling techniques were carried out using yellow traps and tools in the form of swing nets. From the sampling results found 7 species of pests and 4 species of natural enemies in intercropping corn and peanuts in Oemasi Village, The most pest population found in intercropping corn and peanuts is Helicoverpa armigera and the least is Anoplocnemis phasiana The population of natural enemies found in intercropping corn and peanuts is Solenopsis geminata, The diversity index of insect pests and natural enemies in intercropping corn and peanuts is included in the medium category.

Tingkat Kerusakan Buah Kopi oleh Penggerek Buah Kopi (Hypothenemus hampei) di Desa Watumbelar Kecamatan Lewa Tidahu Kabupaten Sumba Timur Titik Sri Harini; Zainal Abidin
Wana Lestari Vol 6 No 1 (2024): Wana Lestari
Publisher : Program Studi Kehutanan, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/wanalestari.v6i01.17202

This research aims to determine the level of damage to coffee cherries caused by the coffee berry borer (Hypothenemus hampei) in Watumbelar Village, Lewa Tidahu District, East Sumba Regency, on coffee plantations owned by farmers in Watumbelar Village, Lewa Tidahu District, Sumba Regency. East. The method used in this research is a survey method with direct observation techniques on coffee plants to obtain data on the development of damage intensity and the level of damage caused by coffee berry borers. To observe the intensity of damage, 5 coffee farmers/land owners were determined or selected. The area of each land/coffee plantation used as a sample plot is 1 ha. Observations were carried out using the diagonal method, and each plot consisted of 5 sub plots measuring 5x5m². From the land area/expanse of coffee plantations owned by 5 farmers, the number of all plants as observation samples for each farmer's garden was 25 trees, so the total number of coffee plants observed was 125 trees. Observations were carried out 8 times with an observation interval of 1 time a week, and carried out in the morning (07.00 WITA-finish). Symptoms of coffee fruit damage caused by H. hampei in Watumbelar Village are that the coffee

Identifikasi Serangga Hama pada Tanaman Kopi Arabika S795 di Desa Beiwali Kecamatan Bajawa Kabupaten Ngada Titik Sri Harini; Zainal Abidin
Wana Lestari Vol 6 No 2 (2024): Wana Lestari
Publisher : Program Studi Kehutanan, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/wanalestari.v6i2.20082

This research was carried out in Beiwali Village, Bajawa District, Ngada Regency. The aim of this research was to identify the types of pest insects, symptoms of damage caused by pests, the population of pest insects on arabica S795 coffee plants in Beiwali Village, Bajawa District, Ngada Regency. The method used in this research is a survey method. The types of data collected are primary data and secondary data. Primary data was obtained by conducting interviews with farmers at the research location and direct observation of arabica S795 coffee plants and using traps in the form of coptan attractants and yellow traps, while secondary data including rainfall, temperature and air humidity were obtained from the research location villages and other agencies. The pest insects obtained were collected, grouped and put into bottles and collection boxes to be preserved and identified at the Plant Pest Laboratory, Faculty of Agriculture, Nusa Cendana University. The results of this research show that the types of insect pests found and the symptoms of damage caused to Arabica coffee plants are: Hypothenemus hampei with symptoms of damage: holes at the tip of the coffee fruit, cracks on the inside which causes the coffee fruit to turn black and become damaged. Planococcus citri with symptoms of fruit slowly turning yellow and black sooty mold appearing. Meanwhile, Aphis gossyipii was found in colonies under plant shoots which were below the surface of the leaves but no signs of significant damage were found. The pest insect populations found were: Hypothenemus hampei with a total of 184 individuals, Planococcus citri with a total of 166 individuals and Aphis gossyipii with a total of 32 individuals. The highest percentage of damage intensity was caused by Hypothenemus hampei with a cumulative average of 14.13%, which was included in the mild category. The cumulative average intensity of damage by Planococcus citri was 0.88%, which was included in the mild category, while there was no damage to Aphis gossyipii.

Inhibitory response of Pectobacterium carotovorum subsp. carotovorum(Jones) Dye Applied with Betel Leaf and Areca Nut Extract as a Potential Organic Bactericide Emerensiana - Uge; Titik Sri Harini; Sri Widinugraheni; Jilinda B.D Henuk
Cannarium Vol 18 No 1 (2020)
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/cannarium.v18i1.1451

Soft root caused by the bacterium Pectobacterium carotovorum subsp carotovorum (Jones) Dye (P.c.c), is a disease that is very detrimental to horticultural plants, especially carrot. The disease control is commonly done by chemical techniques, but because ofÃ‚Â the negative impact, it is important to choose an environmentally friendly techniques, such as plant extracts. The aim of this research is to know response of P.c.c bacteria growth when it is applied with betel leaves extracts (BTL) and Areca nut extracts (ANE) in different concentrations.This research is begun Ã‚Â with pathogenecity test,Ã‚Â Gram coloring test, and Growing on selective media. While the inhibation testing was carried out in In Vitro. Experiment were carried out with agarose-wells method, where bacterial inoculum was grown with agarose medium, then plant extract added in to medium through the well. The observation done by measured inhibitory zone diameter (mm) around agarose-wells. The data presented the size of the inhibitory zone diameter (mm) formed on P.c.c culture media, then was analyzedÃ‚Â usingÃ‚Â ANOVA followed byÃ‚Â DuncanÃ¢â‚¬â„¢sÃ‚Â test.Pathogenecity test, Gram-coloring test, and growing selective media test showed that there was symptoms on 4 days after infection, red and rod-shaped bacteria, while in CVD medium, the bacteria grow uniformly at 37 0 C, although they do not form a basin in the medium. And In vitro test showed that the treatment of BTL, and ANEÃ‚Â at 10%, 5%, and 10% concentration respectively, had good ability to inhibit bacteria in NA medium, with the average inhibition zone diameters of 32.33 mm, 28.67 mm, and 33 .67 mm.

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