Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal SOCA: Jurnal Sosial Ekonomi Pertanian Cannarium (Jurnal Ilmu-ilmu Pertanian) Jurnal Perlindungan Tanaman Indonesia
Sri Widinugraheni
Faculty Of Agriculture, Nusa Cendana University, Jln. Adisucipto, Penfui-Kupang, Nusa Tenggara Timur 85228
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Social Sciences

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Evaluation of Some Specific Primer Sets Development for Detecting Fusarium oxysporum f. sp. cubense Tropic Race 4 (Foc TR4) Originating from Indonesia Yudha Pratama; Arif Wibowo; Ani Widiastuti; Siti Subandiyah; Sri Widinugraheni; Martijn Rep
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.25037

Abstract

Fusarium oxysporum f. sp. cubense tropic race 4 (Foc TR4) strain which belong to Vegetative Compatibility Group (VCG) 01213 is the most devastating disease in global banana production. Validation of specific primer sets using the positive control (Foc TR4). In total, 50 isolates of Foc are collected from several banana production regions in Indonesia represent the group of VCG, races, genotype, cultivars, which are confirmed as Foc based on the tested using FocEf3 primer set, except Cjr-2 and Lmp-4 isolates. Foc-1/Foc-2 could amplify 34 Foc isolates included in Foc race 4. Three specific primer sets i.e. TR4-F/TR4-R, Six-1c, and TR4-F2/TR4-R1 are used to classify Foc isolates into Foc tropic race 4. TR4-F/TR4-R is known have the highest specificity as it could amplify 35 Foc isolates including positive controls (Foc TR4) compared to the other primer sets (Six-1c and TR4-F2/TR4-R1). This research indicates that there are a large number of diversity strains found in Foc isolates to be studied for further research. Race 4 of Foc (STR4 or TR4) is known to be widespread in several regions in Indonesia. Therefore, specific primer set development needs to be done to detect Foc TR4 and the most damaging strains on Foc TR4 based on molecular data.IntisariFusarium oxysporum f. sp. cubense ras 4 tropika (Foc TR4) yang termasuk ke dalam kelompok VCG 01213 merupakan patogen yang paling merusak dalam produksi tanaman pisang secara global. Validasi primer spesifik berbasis PCR menggunakan kontrol positif (Foc TR4). Total, 50 isolat Foc dikoleksi dari  beberapa daerah produksi pisang di Indonesia mewakili VCG, ras, genotipe dan kultivar yang dikonfirmasi sebagai isolat Foc berdasarkan pengujian menggunakan primer FocEf3, kecuali isolat Cjr-2 dan Lmp-4. Foc-1/Foc-2 dapat mengamplifikasi 34 isolat Foc yang termasuk ke dalam Foc ras 4. Selanjutnya tiga pasang primer spesifik yaitu TR4-F/TR4-R, Six-1c, dan TR4-F2/TR4-R1 digunakan untuk mengelompokkan isolat-isolat tersebut ke dalam isolat Foc ras 4 tropika. TR4-F/TR4-R diketahui memiliki spesifitas tertinggi karena dapat mengamplifikasi sebanyak 35 isolat Foc termasuk kontrol positif (Foc TR4) dibandingkan dengan primer lainnya (Six-1c dan TR4-F2/TR4-R1). Penelitian ini menunjukkan bahwa terdapat sejumlah besar keragaman strain yang terlihat pada isolat-isolat Foc tersebut untuk dapat dipelajari lebih lanjut. Ras 4 dari Foc (STR4 atau TR4) diketahui tersebar luas pada beberapa daerah di Indonesia. Oleh karena itu, perlu dilakukan pengembangan primer spesifik untuk mendeteksi Foc TR4 dan strain yang paling merusak pada Foc TR4 berdasarkan data molekuler.
The KoBoCollect for Research Data Collection and Management (An experience in Researching the Socio-Economic Impact of Blood Disease in Banana) I Wayan Nampa; I Wayan Mudita; Norman Patrick Lucky Bire Riwu Kaho; Sri Widinugraheni; Remi Lasarus Natonis
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 14 No 3 (2020): Vol.14, No.3, 2020
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.484 KB) | DOI: 10.24843/SOCA.2020.v14.i03.p15

Abstract

In the research field, smartphone began to be used for field data collection. One application that was used was KoBoCollect. This article shared the experience of using KoBoCollect in research on the socio-economic impact of blood diseases on banana in Sumba Island. The research was conducted by interviewing 64 banana farmers that spread proportionally in four regencies in Sumba Island. The use of KoBoCollect/toolbox made it easy to collect, save and manage research data. KoBoCollect/toolbox provided the advantages of conducting paperless research, able to record data in complex formats such as numeric data, descriptive data, photos, videos, sounds, coordinates and other data, dynamically making forms changes when conducting research, and displaying interactive applications. KoBoCollect can be run off-line, made it possible to collect field data in remote locations and no internet network. The research data was transmitted online so that the data tabulation process was automatic. Simple data analysis such as median values, mode, graphs and distribution maps of data collection were provided in KoBotoolbox. With KoBoCollect/toolbox, researchers quickly find out the results of the research, as in this research it was known that blood disease had an impact on the decrease in the banana population revealed by 81.54% of farmers.
Inhibitory response of Pectobacterium carotovorum subsp. carotovorum(Jones) Dye Applied with Betel Leaf and Areca Nut Extract as a Potential Organic Bactericide Emerensiana - Uge; Titik Sri Harini; Sri Widinugraheni; Jilinda B.D Henuk
Cannarium Vol 18, No 1 (2020)
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.961 KB) | DOI: 10.33387/cannarium.v18i1.1451

Abstract

Soft root caused by the bacterium Pectobacterium carotovorum subsp carotovorum (Jones) Dye (P.c.c), is a disease that is very detrimental to horticultural plants, especially carrot. The disease control is commonly done by chemical techniques, but because of  the negative impact, it is important to choose an environmentally friendly techniques, such as plant extracts. The aim of this research is to know response of P.c.c bacteria growth when it is applied with betel leaves extracts (BTL) and Areca nut extracts (ANE) in different concentrations.This research is begun  with pathogenecity test,  Gram coloring test, and Growing on selective media. While the inhibation testing was carried out in In Vitro. Experiment were carried out with agarose-wells method, where bacterial inoculum was grown with agarose medium, then plant extract added in to medium through the well. The observation done by measured inhibitory zone diameter (mm) around agarose-wells. The data presented the size of the inhibitory zone diameter (mm) formed on P.c.c culture media, then was analyzed  using  ANOVA followed by  Duncan’s  test.Pathogenecity test, Gram-coloring test, and growing selective media test showed that there was symptoms on 4 days after infection, red and rod-shaped bacteria, while in CVD medium, the bacteria grow uniformly at 37 0 C, although they do not form a basin in the medium. And In vitro test showed that the treatment of BTL, and ANE  at 10%, 5%, and 10% concentration respectively, had good ability to inhibit bacteria in NA medium, with the average inhibition zone diameters of 32.33 mm, 28.67 mm, and 33 .67 mm.
Co-Authors Ani Widiastuti Arif Wibowo Emerensiana - Uge I Wayan Mudita Jilinda B.D Henuk Martijn Rep Nampa, I Wayan Norman Patrick Lucky Bire Riwu Kaho Remi Lasarus Natonis Siti Subandiyah Titik Sri Harini Yudha Pratama