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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Eruditio : Indonesia Journal of Food and Drug Safety
Kemala S. Nagur
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Penerapan ISO 16140-3:2021 untuk Pencacahan Enterobacteriaceae pada Produk Pangan Nagur, Kemala S.; Surati, Sri; Marusaha Sitorus , Aditya Anugerah; Cahyaningsih, Eni; Putri, Fannisa; Wilasti, Yulin
Eruditio : Indonesia Journal of Food and Drug Safety Vol 4 No 1 (2023): Edisi Desember
Publisher : Badan Pengawas Obat dan Makanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54384/eruditio.v4i1.195

Abstract

Method verification is one of the steps required to ensure that all methods used meet the standards required for data reliability and accuracy in a laboratory that implements Good Laboratory Practices (GLP). In accordance with ISO 16140-3:2021, the method verification for microbiology testing implemented by calculating the Intralaboratory Reproducibility Standard Deviation (SIR) for implementation verification and eBias value for food item verification. Method verification for enumeration of Enterobacteriaceae in food products using ISO 16140-3 has never been carried out at the Indonesian Food and Drug Authority (FDA) laboratories. Therefore, verification of this method needs to be performed. This study aimed to verify the ISO 21528-2:2017 as reference method for enumeration of Enterobacteriaceae in food products by colony count technique. The selected food item for implementation verification was pasteurized milk while for food item verification, the selected food items were yogurt, traditional preserved egg (telur pindang), sweet and hard candy, seasoning dry powder, worcester sauce and cassava chips. All of selected foods were artificially contaminated with Escherichia coli WDCM 00012. The SIR value obtained was 0.12 ≤ 2× 0,18 (lowest mean value of SR from ISO 21528-2:2017), indicating that the acceptability limit for the implementation verification was met. While the eBias value for all selected food item were less than 0,5 log10, thus the verification study in the National Quality Contol Laboratory of Drug and Food (NQCLDF), Indonesian FDA, meets the acceptance criteria defined in the standard ISO 16140-3:2021.
Identification of Aspergillus flavus and Detection of Its Aflatoxin Genes Isolated from Peanut and Peanut Processed Products Nagur, Kemala S.; Sukarno, Nampiah; Listiyowati, Sri
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Co-Authors Cahyaningsih, Eni Fannisa Putri Marusaha Sitorus , Aditya Anugerah Nampiah Sukarno Sri Listiyowati Surati, Sri Wilasti, Yulin