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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science
Arning W. Ekawati
Faculty of Fisheries and Marine Sciences, University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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ICP11 as Biomarker for WSSV Disease in Litopenaeus vannamei Yuni Kilawati; Yunita Maimunah; Arning W. Ekawati
Research Journal of Life Science Vol 2, No 3 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.35 KB) | DOI: 10.21776/ub.rjls.2016.002.03.5

Abstract

Environmental pollution could directly reduce water quality for shrimp cultivation. This study aims to determine how the environmental quality, shrimp population and genetic characteristics of shrimp that live in polluted waters in shrimp ponds in East Java associated with WSSV disease that often attacks on cultivated shrimp. The method used was a descriptive exploratory. Data collection was collected by observation and interview with farmers on disease history. Water was sampled for water quality study and characterize its suitability for shrimp cultivations. Shrimps were also sampled for morphological and genetical study for its susceptibility of WSSV. All samples were taken every week during shrimp cultivation in Malang ans Gresik. Morphological study using scoring method to determine the disease stages on shrimp, while for genetical study using specific primer for ICP11 for WSSV detection, since ICP 11 is expressed when WSSV infection is occur. Samples were taken from 2 shrimp ponds in South Malang and Gresik.The overall water quality is good, except for NO2 and TOM in both seawater shrimp ponds which higher than the freshwater/estuary shrimp ponds. Light infections of WSSV were detected in all seawater ponds both in morphology and genetics. However, in the freshwater/estuary pond only shrimps from freshwater/estuary Gresik which showing light WSSV infection genetically, but not in the morphological signs. Early disease detection is important to control the disease spread.
Dried Skeletonema costatum in Feed Formulation for the Growth of Vaname Shrimp (Litopenaeus vannamei) Dewi Putri Lestari; Arning W. Ekawati; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (914.365 KB) | DOI: 10.21776/ub.jels.2014.004.02.04

Abstract

The aim of this study was to determine the effect and the best dosage used by the Skeletonema costatum in feed formulation on the growth of Vaname shrimp. This experiment used Completely Randomized Design (CRD) with four treatments and three replications. Treatment was given by using dried S. costatum in feed formula (iso protein 37% and iso energy 3.6 kkal/g feed). Feed treatment was the substitution of fish meal protein A = 0%, B = 2.5%, C = 5% and D = 7.5% with dried S. costatum protein. The observed parameters include Survival Rate (SR), Survival Growth Rate (SGR), Feed Efficiency Ratio (FER), and Protein Efficiency Ratio (PER). The results showed that the use of dried S. costatum in feeding are effective in increasing the specific growth rate, feed efficiency ratio and the ratio of protein efficiency than controls. The best dosage in feeding formula is ranged from 4.39% to 4.75%.Keywords: feeding formula, Skeletonema costatum, Vaname Shrimp
Co-Authors Dewi Putri Lestari Maftuch Maftuch Yuni Kilawati Yunita Maimunah