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Genetic Characteristics Of White Shrimp (Litopenaeus Vannamei) In Banyuwangi And Situbondo: The Infected White Spot Syndrome Virus (WSSV) kilawati, yuni; Maimunah, Yunita
Journal of Food and Life Sciences Vol 2, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6.551 KB) | DOI: 10.21776/ub.jfls.2018.002.02.04

Abstract

Water pollution caused by human anthropogenic activities has deteriorating effect on water quality for aquaculture. Therefore, the objectives of this research were to determine how water quality, population and shrimp genetic characteristic in polluted area. Especially in East Java which have high virulency on WSSV outbreaks.Data exploration using surveillance technique was collecting physical and chemical water quality parameters. Shrimp samples were analyzed for morphological and genetical characteristics using specific WSSV primer ICP 11.Water quality at all sampling locations were categorized as polluted refers to ammonia and TOM  concentrations. Low water quality acts as a trigger for WSSV outbreaks. Shrimp pond from Situbondo exhibited as carrier indicated by the presence of WSS infection based on genetic characteristics, while morphological characteristics did not.
Kualitas Lingkungan Tambak Insentif Litapenaeus Vannamei Dalam Kaitannya Dengan Prevalensi Penyakit White Spot Syndrome Virus Maimunah, Yunita; Kilawati, Yuni
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.254 KB) | DOI: 10.21776/ub.rjls.2015.002.01.7

Abstract

Increasing number of vannamei shrimp (Litapenaeus vannamei) ponds are switching from traditional to intensive farming systems, the more impact resulting among other potential environmental pollution. Pollution of the environment can directly degrade water quality cultivation and facilitate access of pathogens to infect the host. In this study examines how the quality of the environment, population and genetic characteristics of shrimp that live in some intensive pond associated with a disease that often affects farmed shrimp is White Spot Syndrome Virus (WSSV). Acquisition and primary data collection is done by conducting interviews and direct observation in the measurement of water quality parameters of both physics and chemistry and morphology observation of shrimp as well as the ICP11 gene expression detection of WSSV disease in vannamei shrimp DNA in the laboratory.
Performance of Growth in Tilapia Fish in Polyculture System Maimunah, Yunita
Journal of Food and Life Sciences Vol 4, No 1 (2020)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfls.2020.004.01.05

Abstract

Tilapia growth in pond using polyculture system is higher than tilapia cultured in monoculture system. Employing water column for fish and prawn culture to obtained higher production yield. This research using experimental descriptional method and data were analyzed using independent t-test, comparing monoculture and polyculture of Tilapia growth performance. Specific growth rate of polyculture Tilapia at 4.46%/BW/day is higher than monoculture fish at 4.80%/BW/day. FCR comparison  were 1,1 and 1.17 for polyculture and monoculture respectively. Water quality treatments were at optimal range for Tilapia growth at 26-30C; 7,34-8,36; and 5,3-8,8  ppm for temperature, pH dan dissolved oxygen, respectively.
Memanfaatkan Internet of Aquaculture dalam Meningkatkan Kualitas Produksi pada Kelompok Pembudidaya Ikan Koi di Blitar Dany Primanita Primanita Kartikasari; Adhitya Bhawiyuga; Yuni Kilawati; Yunita Maimunah; Adharul Muttaqin
Journal of Innovation and Applied Technology Vol 7, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

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Abstract

Koi fish farming is growing because of the stable selling value and the number of fans. One area that is geographically suitable for developing Koi fish farming is Blitar. The production of Koi fish produced by cultivators in Blitar is known to have quality that is not inferior to Koi from other countries so that consumers are spread all over the world. This disease is thought to originate from the decline in environmental quality that is not handled properly, causing a decrease in the Koi fish's immune system. The purpose of this activity is to provide education and assistance on efforts to prevent mortality that occurs in Koi fish through the provision of natural immunostimulants and water quality control using internet-based tools to Koi fish farmers in Blitar. the use of materials and tools as well as the provision of immunostimulant materials and internet-based water quality detectors.
Implementasi Internet Of Aquaculture (IoA) untuk Deteksi Kualitas Lingkungan Secara Cepat dalam Upaya Pemberdayaan Kelompok Pembudidaya Ikan Koi Di Blitar Yuni Kilawati; Yunita Maimunah; Adharul Muttaqin
Journal of Innovation and Applied Technology Vol 6, No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2020.006.02.14

Abstract

The increasing number of Koi ornamental fish interested around the world, this fish farming business is increasingly developing. Blitar is one of the geographically suitable areas to develop Koi fish farming. The resulting production has a quality  of Koi better than from other countries. Cultivation models and techniques developed are also increasingly advanced, but often cultivators have disease problems stemming from decreasing environmental quality that are not handled early. This attracted the Brawijaya University  Doctoral community service team to help find solutions to these problems.
Kajian Tanah Dasar Tambak Ikan Bandeng (Chanos chanos Forsk) di Desa Curahsawo, Kecamatan Gending, Kabupaten Probolinggo, Jawa Timur Yunita Maimunah; Dedy Novianto; Purwohadijanto Purwohadijanto
Indonesian Journal of Environment and Sustainable Development Vol 2, No 1 (2011)
Publisher : Graduate Program

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Abstract

Pengetahuan tentang kualitas tanah dasar tambak sangat penting sebagai dasar dalam upaya peningkatan produksi ikan bandeng. Kualitas tanah antara lain perubahan fisika, kimia dan biologi tanah dasar tambak menurun sejalan dengan waktu penggunaan tambak. Berdasarkan hal tersebut perlu dilakukan penelitian untuk mengkaji kualitas tanah tambak ikan bandeng (Chanos chanos Forsk) di sentra budidaya ikan Bandeng di Desa Curahsawo, Kecamatan Gending, Kabupaten Probolinggo. Penelitian ini dilaksanakan pada tambak di Desa Curahsawo, Kecamatan Gending, Kabupaten Probolinggo, Laboratorium Fisika dan Biologi Tanah Fakultas Pertanian, dan Laboratorium Sentra Ilmu Hayati (LSIH) Universitas Brawijaya pada bulan November 2010 sampai Januari 2011. Metode yang digunakan adalah metode penelitian deskriptif  untuk mencari informasi faktual yang mendetail yang mendeskripsikan gejala yang ada serta mengidentifikasi permasalahan untuk mendapatkan data. Parameter yang diuji meliputi parameter fisika, kimia dan biologi. Hasil penelitian menunjukkan bahwa berdasarkan kualitas tanah, tambak 1, 2 dan 3 layak digunakan untuk budidaya ikan bandeng (Chanos chanos Forsk) tetapi, masih perlu penambahan unsur hara untuk meningkatkan kesuburan tanah melalui pemupukan. Tambak 1 dan 2 tergolong paling subur sedangkan tambak 3 memiliki tingkat kesuburan sedang. Perlu perbaikan pengelolaan tanah dan pemupukan untuk meningkatkan kandungan unsur hara Nitrogen (N) serta Posfor (P) dalam tanah. Kata kunci: biologi, deskriptif, fisika, kimia, tanah, unsur hara
ICP11 as Biomarker for WSSV Disease in Litopenaeus vannamei Yuni Kilawati; Yunita Maimunah; Arning W. Ekawati
Research Journal of Life Science Vol 2, No 3 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.35 KB) | DOI: 10.21776/ub.rjls.2016.002.03.5

Abstract

Environmental pollution could directly reduce water quality for shrimp cultivation. This study aims to determine how the environmental quality, shrimp population and genetic characteristics of shrimp that live in polluted waters in shrimp ponds in East Java associated with WSSV disease that often attacks on cultivated shrimp. The method used was a descriptive exploratory. Data collection was collected by observation and interview with farmers on disease history. Water was sampled for water quality study and characterize its suitability for shrimp cultivations. Shrimps were also sampled for morphological and genetical study for its susceptibility of WSSV. All samples were taken every week during shrimp cultivation in Malang ans Gresik. Morphological study using scoring method to determine the disease stages on shrimp, while for genetical study using specific primer for ICP11 for WSSV detection, since ICP 11 is expressed when WSSV infection is occur. Samples were taken from 2 shrimp ponds in South Malang and Gresik.The overall water quality is good, except for NO2 and TOM in both seawater shrimp ponds which higher than the freshwater/estuary shrimp ponds. Light infections of WSSV were detected in all seawater ponds both in morphology and genetics. However, in the freshwater/estuary pond only shrimps from freshwater/estuary Gresik which showing light WSSV infection genetically, but not in the morphological signs. Early disease detection is important to control the disease spread.
Penggunaan Ekstrak Daun Lamtoro (Leucaena Leucocephala) Sebagai Pupuk Dengan Salinitas Yang Berbeda Terhadap Laju Pertumbuhan, Biomassa Dan Klorofil-A Pada Mikroalga Chlorella Vulgaris Ana Evita Aulia; Yunita Maimunah; Heny Suprastyani
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 1 (2021): JFMR VOL 5 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.8

Abstract

Kultur Chlorellah vulgaris umumnya menggunakan pupuk Pro Analisis (PA), mahalnya harga pupuk PA menjadi dasar pencarian pupuk alternatif yang mampu memenuhi kebutuhan nutrisi C. vulgaris. Pupuk alternatif tersebut yaitu pupuk organik berbahan baku daun lamtoro (L. Leucocephala) yang diekstrak. Tujuan dari penelitian ini yaitu mengetahui mengetahui pengaruh perbedaan salinitas dan mengetahui nilai salinitas yang optimal pada pupuk ekstrak daun lamtoro untuk laju pertumbuhan, biomassa dan klorofil-a C.  vulgaris. Metode yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan. Perlakuan yang diberikan pada penelitian ini adalah pupuk ekstrak daun lamtoro (L. Leucocephala) dosis 1,75 ml/l dengan salinitas 30 ppt, 35 ppt, 40 ppt dan kontrol (walne 1 ml/l) salinitas 30 ppt. Parameter utama yang diamati yaitu pertumbuhan, biomassa dan klorofil-a C. vulgaris. Hasil penelitian menunjukkan bahwa pemberian pupuk ekstrak daun lamtoro (L. Leucocephala) dengan salinitas yang berbeda berpengaruh nyata terhadap pertumbuhan, biomassa dan klorofil-a C. vulgaris. Perlakuan terbaik selama penelitian didapatkan hasil laju pertumbuhan spesifik 1,94/hari pada salinitas 35 ppt, biomassa sebesar 0,403 g/l pada salinitas 35 ppt dan klorofil-a sebesar 0,018 µg/ml pada salinitas 35 ppt. Berdasarkan hasil penelitian ini, disarankan agar menggunakan salinitas 34 untuk penelitian lanjutan sesuai dengan hasil maksimum agar mendapatkan hasil yang lebih baik.
HIBRIDISASI INTERSPESIFIK TIGA SPESIES IKAN TOR (Tor soro, Tor douronensis, DAN Tor tambroides) SECARA RESIPROKAL PADA FASE LARVA Toni Yulian Kusmardani; Otong Zenal Arifin; Agoes Soeprijanto; Yunita Maimunah
Jurnal Riset Akuakultur Vol 16, No 1 (2021): (Maret, 2021)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.16.1.2021.1-8

Abstract

Ikan Tor merupakan genus yang potensi dibudidayakan karena memiliki nilai ekonomis tinggi. Masalah yang ada adalah lambatnya pertumbuhan dan rendahnya tingkat sintasan benih ikan tersebut dalam jumlah yang memadai untuk pembudidaya. Hibridisasi atau persilangan merupakan suatu upaya untuk mendapatkan kombinasi antara populasi yang berbeda untuk menghasilkan keturunan yang memiliki sifat unggul. Hibridisasi tiga spesies ikan Tor secara resiprokal antara Tor soro (Ts), Tor douronensis (Td), dan Tor tambroides (Tt) dilakukan guna untuk mengetahui keberhasilan hibridisasi beda spesies tetapi masih dalam genus. Metode yang digunakan pada penelitian ini adalah eksperimen dengan enam perlakuan dan tiga kali ulangan hasil hibrida. Hasil yang diperoleh menunjukkan nilai derajat pembuahan tertinggi dihasilkan dari hibridisasi Td >< Ts (93,66 ± 1,15%), daya tetas tertinggi dihasilkan dari persilangan Td >< Ts (88,00 ± 1,00%), laju pertumbuhan spesifik bobot tertinggi dihasilkan dari hibridisasi Ts >< Td dan Ts >< Tt (5,62 ± 0,17%; 5,44 ± 0,06%), laju pertumbuhan spesifik panjang tertinggi dihasilkan dari hibridisasi Ts >< Td (1,79 ± 0,11%) dan sintasan tertinggi dihasilkan dari hibridisasi Tt >< Ts (86,00 ± 3,60%). Nilai heterosisyang meliputi derajat pembuahan, daya tetas telur, pertumbuhan benih, dan sintasan ikan sampai umur 41 hari menunjukkan nilai negatif, kecuali laju pertumbuhan spesifik bobot. Hibridisasi berdampak pada meningkatnya jumlah larva abnormal yang dihasilkan dibanding tetuanya (pure breed).Tor fish has the potential to be commercially developed as a farmed fish due to its high economic value and market demand. However, slow growth and low survival rate of the fish larvae and insufficient seed availability for aquaculture farmers are the main bottlenecks to develop a profitable aquaculture for tor fish. Hybridization or crossbreed is an attempt to get a combination between different populations to produce offspring inheriting superior characters. A reciprocal hybridization between Tor soro (Ts), Tor douronensis (Td), and Tor tambroides (Tt) was carried out to assess the success rate of hybridization between the three species. The study used an experimental design with six treatments and three replications to measure and compare hybridization and survival parameters between the species. The highest fertilization rate attained by hybridization Td >< Ts (93.66 ± 1.15%, the highest hatching rate was achieved by Td >< Ts (88.00 ± 1.00%), the highest weight specific growth rate was gained by hybridization Ts >< Td and Ts >< Tt (5.62 ± 0.17%; 5.44 ± 0.06%), the highest lenght specific growth rate was achieved by hybridization Ts >< Td (1.79 ± 0.11%) and highest survival rate was resulted from hybridization between Tor tambroides >< Tor tambroides (90.33 ± 1.52%). The observed heterosis (degree of fertilization rate, hatching rate, growth rate, and survival rate until the age of 41 days) showed that all parameter characters were negative, except for the weight-specific growth rate characters. This study concludes that hybridization have an impact on increasing the number of abnormal larvae produced compared to their parents (pure-breed).
Survival Rate Evaluation of Different Filler Medium of Waterless Live Fish Transportation of African Catfish (Clarias sp.) Broodstock Ayuningtyas Ayuningtyas; Baruna Kusuma; Abdul Rahem Faqih; Yunita Maimunah
Journal of Aquaculture Development and Environment Vol 1, No 1 (2018): Journal Of Aquaculture Development And Environment
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jade.v1i1.1012

Abstract

The supplying of live fish broodstock is routinely delivered by water. However, the traditional transportation of live fish with water results in small volume of transportation. Therefore, waterless transportation with different filler medium of waterless live fish transport is considered an alternative strategy. This research aimed to evaluate the effect of different filler medium of waterless live fish transportation on survival rate of African catfish (Clarias sp.) broodstocks. Brooders with mean weight 116 ±10,25 g were packed at 5 fish/bag for simulated 15h transportation. The bags, each 40x25x15 cm3, were filled with different treatment of medium; Sponge, newspaper, cloth, and sawdust at 3 cm thickness of sponge medium and ½ kg for newspaper, cloth and sawdust medium per bag. One bag without medium was designed as a control. Mortality, temperature, Humidity and weight gain loss were monitored throughout the experiment. After simulation, mortality, pH, DO and temperature were monitored in fiber pond over 14 days. Effect of different treatment of medium was significantly different (p < 0.05) with highest survival (100%) at sponge and cloth medium, and lowest survival (24 %) at sawdust medium. Humidity of newspaper medium and weight gain loss of sawdust medium significantly decreased and no significantly different in temperature parameter after simulated 15h transportation. It was concluded that sponge and cloth medium might be applied as a filler medium for waterless live fish transportation.