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Aswaty Nur
Department Of Physiology, Faculty Of Medicine, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Effect of Sweet Purple Potato Extract (Ipomoea batatas L) on Zebrafish (Danio rerio) by Diet Induced Obesity Aswaty Nur; Yhusi Karina Riskawati; Edwin Widodo; Retty Ratnawati; Amirah Firdausy; Lydia Kusuma Dewi; Maria Karina
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.637 KB) | DOI: 10.21776/ub.rjls.2019.006.02.2

Abstract

The effect of a high-fat diet on zebrafish as an alternative experimental animal in studying lipid metabolism is not fully understood. In this research, sweet purple potato extract (SPPE) which has anthocyanin have used as an anti-obesity property. Zebrafish aged 8 mpf was divided into five groups: Non-DIO (control negative), DIO (Diet-Induced Obesity, control positive), and three experimental groups (DIO A, DIO B, DIO C). Each group consisted of five Zebrafish. All groups have been fed freshly hatched Artemia for 40 days where the Non-DIO’s amount was 5 mg Cyst/fish one time per day, and the other groups’ amount was 60 mg Cyst/fish three times per day. The experimental groups were exposed to sweet purple potato extract three times per day by different SPPE doses: DIO A by 80 ppm, DIO B by 120 ppm, and DIO C by 160 ppm. The results show that there was no significant difference between the positive control group and the experimental groups in term of feeding intake, BMI, blood glucose, and total cholesterol. However, based on molecular parameters, there was a declining trend on PPAR-γ level, although, the adiponectin level remained stable. We suggest that anthocyanin does not affect feed intake, BMI, blood glucose level, cholesterol, and expression of PPAR-γ and adiponectin of zebrafish with diet-induced obesity.
Catechins of GMB-4 Clone Inhibits Adipogenesis Through PPAR-γ and Adiponectin in Primary Culture of Visceral Preadipocyte of Rattus Norvegicus Wistar Aswaty Nur; Retty Ratnawati; Diana Lyrawati
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.852 KB) | DOI: 10.21776/ub.rjls.2018.005.01.6

Abstract

Catechins of green tea (Camelia sinensis) GMB4 clone may serve as a potential therapeutic antiobesity agent, probably through its effects on preadipocytes. Thus, to evaluate such antiobesity effects, we performed series of in vitro experiments using primary cultures of visceral preadipocytes from Rattus norvegicus strain Wistar. Quick Cell Proliferation assay, Oil Red-O staining, ELISA and immunocytochemistry were used to determine the effects of 25 µM, 50 µM, 75 µM, and 100 µM catechins on primary culture of preadipocytes, particularly on cell viability and differentiation as well as on expression of relevant obesity genes i. e. PPARγ and adiponectin levels. The results showed that there were no significant differences on preadipocytes viability among control and catechins treatments except in cells treated with 50 µM catechins (means±SD=128±2.47) which resulted 28% higher viability than control (p= 0.037). Catechins inhibited preadipocytes differentiation into adipocytes, at 100 µM up to 78% lower than control. The level of PPARy apparently was reduced by catechins, but statistically significant only at 75 μM (p= 0.029). In contrast, the adiponectin level on preadipocytes increased by catechins at 75 µM and 100 µM (0.786±0.126 and 0.673 ±0.319 ng/ml; control: 0.077±0.017, p ≤0.01). In conclusions, our data revealed that desired antiobesity effects of catechins of green tea GMB4 clone on visceral preadipocytes were concentration-dependent; at dosage 50 µM catechins enhanced cell viability; at more than 75 µM inhibited differentiation of preadipocytes and was associated with lower PPARy and higher adiponectin levels.
Co-Authors Amirah Firdausy Diana Lyrawati Edwin Widodo Lydia Kusuma Dewi Maria Karina Retty Ratnawati Retty Ratnawati Riskawati, Yhusi Karina