Garuda - Garba Rujukan Digital

Tri Ardyati

University of Brawijaya

Author-ID : 3280531

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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1

Polytope Prediction for Dengue Vaccine Candidate Based on Conserved Envelope Glycoprotein of Four Serotypes of Dengue Virus and Its Antigenicity Karimatul Himmah; Fitriyah Fitriyah; Tri Ardyati; Custer Deocaris; Nashi Widodo
The Journal of Pure and Applied Chemistry Research Vol 5, No 2 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Dengue fever reported endemic in tropical and sub-tropical country. Dengue fever caused by dengue virus, has Envelope protein that often used for vaccine development to prevent the virus infection. Vaccine development to prevent four serotype dengue virus infection still unavailable. This study aims to design polytope from four conserved epitopes of dengue virus envelope glycoprotein to prevent infection of heterotypic dengue virus and predict its antigenic challenge by molecular docking. We investigate molecular modeling of polytope, immunoinformatics analysis of polytope, protein structure of antibodies, molecular docking and protein-protein docking assessment. The polytope categorized as a stabil protein with index 29.72, has molecular weight 6,139 kDa, has three exposed antigenic determinants region and has estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro),10 min (yeast, in vivo), and >10 hours (Escherichia coli, in vivo). The Polytope binds with four broadly neutralizing antibodies of B7, C8, A11, and C10 (bnAbs) which estimated that four bnAbs can recognize four serotypes of dengue virus. The designed polytope has prospect to produce in Escherichia coli and can be applied as vaccine of heterotypic dengue virus serotype. Polytope is potentially able to generate humoral and cellular immunity.

Antimicrobial and Antioxidant Activity of Endophyte Bacteria Associated with Curcuma longa Rhizome Sulistiyani Sulistiyani; Tri Ardyati; Sri Winarsih
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Most cases of bacterial resistance towards antibiotics, encourage various efforts to gain new sources of antibiotics. Endophyte bacteria is a microorganism has important role as the producer of bioactive compounds. Endophyte bacteria from Curcuma longa with antimicrobial and antioxidant activities have not been studied yet. Curcuma longa has been utilized as the main ingredients of traditional herbal medicines (jamu). The objective of this research was to investigate the antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. Based on morphological characteristics of bacterial colonies, eight endophyte bacteria was isolated from Curcuma longa rhizome. Screening of endophyte isolate has antimicrobial activity was done using agar well diffusion method. The culture supernatant of each endophyte isolate was dropped on agar well against pathogenic bacteria Salmonella enterica ser. Typhi, Staphylococcus aureus and yeast Candida albicans. Three endophyte isolates K3, K2 and M1b showed antimicrobial activity against pathogenic bacteria and yeast. Isolate K3 showed strong antimicrobial activity against C. albicans and S. aureus, however, isolate K2 and isolate M1b showed antimicrobial activity against Salmonella enterica ser. Typhi and S. aureus, respectively. Those endophyte bacteria also had antioxidant activity shown by scavenging ability toward DPPH radical with consecutive percentage of isolate K3 (72.3 %), K2 (51.3 %) and M1b (64.6 %). Isolate K3 showed the highest antimicrobial and antioxidant activity. Based on biochemical characteristics using Microbact 24E kit, isolate K3 was identified as Paenibacilus alvei and isolate K2 as Enterobacter agglomerans. Keywords: antimicrobial, antioxidant, Curcuma longa's rhizome, endophyte bacteria.

Evaluation of Proteolytic and Chitinolytic Activities of Indigenous Bacillus Species from Crab Shell Waste Moh Dliyauddin; Tri Ardyati; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

The increase of crab meat export activities in Indonesia leads to the accumulation of crab shell waste in a massive amount that can naturally degrade and causing bad odor. Naturally, microorganisms will degrade this waste through fermentation and enzymatic reaction, including protease and chitinase due to its high content of chitin and protein. Bacillus is the most potential bacteria to degrade crab shell waste, and indigenous Bacillus from this waste can increase the degradation rates. The aims of this study were to evaluate the proteolytic and chitinolytic activities of indigenous Bacillus species from crab shell waste. Bacillus cereus BP14 and Bacillus licheniformis CK20 as the chitinolytic bacteria, and Bacillus subtilis AP9 and Bacillus licheniformis AP5 as the proteolytic bacteria were isolated from crab shell waste and identified based on the 16S rDNA sequences. The bacterial isolates were grown in skim milk broth for proteolytic characterization and colloidal chitin broth for chitinolytic characterization. The growth rates of each bacteria were determined through the growth curves. The enzymatic activities were determined based on the international standard for protease and chitinase enzyme activities together with growth curve sampling to determine the best incubation time for obtaining the highest enzymatic activities. From the shortest to the longest generation times of the Bacillus species obtained were B. subtilis AP9, B. licheniformis CK20, B. cereus BP14, and B. licheniformis AP5, respectively. The best incubation time for producing the highest enzymatic activity varied among species. However, the end of the logarithmic phase was similar. All of the Bacillus species obtained from this study exhibited chitinolytic and proteolytic activity. Therefore, it can be used as promising candidates of biodegradation agents inenhancing the degradation rates of crab shell wastes.

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