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All Journal Jurnal Gizi Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) e-Journal Pustaka Kesehatan Pharmaciana: Jurnal Kefarmasian JAM : Jurnal Aplikasi Manajemen Journal of Food and Life Sciences Research Journal of Life Science Plantropica: Journal of Agricultural Science Folia Medica Indonesiana Jurnal Farmasi Klinik Indonesia Mutiara Medika: Jurnal Kedokteran dan Kesehatan MPI (Media Pharmaceutica Indonesiana) Jurnal Sains dan Teknologi Pangan IDEAS: Journal on English Language Teaching and Learning, Linguistics and Literature AL ISHLAH Jurnal Pendidikan Majalah Kesehatan FKUB Medical Laboratory Technology Journal Health Notions Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya JMM (Jurnal Masyarakat Mandiri) Jurnal Teknologi Pangan dan Hasil Pertanian Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) JURNAL PENDIDIKAN TAMBUSAI Indonesian Journal of Natural Pigments PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Pharmaceutical Journal of Indonesia Midwifery and Nursing Research (MANR) Journal of Issues in Midwifery Soedirman Economics Education Journal UTAMAX : Journal of Ultimate Research and Trends in Education Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Food Technology and Halal Science Journal Caring : Jurnal Pengabdian Masyarakat New Language Dimensions Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) BAHTRA: Jurnal Pendidikan Bahasan dan Sastra Maternity and Neonatal : Jurnal Kebidanan Jurnal Kebidanan dan Keperawatan Aisyiyah International Journal of Educational Research Excellence (IJERE) Journal of Artificial Intelligence and Digital Business Jurnal Kedokteran Hewan Jurnal Pendidikan Sosial dan Konseling Science And Education Journal (SNEJ) Scientica: Jurnal Ilmiah Sains dan Teknologi SPIKESNAS Nusantara Journal of Multidisciplinary Science Siber Journal of Transportation and Logistics Buletin Literasi Budaya Sekolah Artikula
Sri Winarsih
Departemen Farmasi Fakultas Kedokteran Universitas Brawijaya
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The Effectiveness Test of Application Anthocyanin’s Extract in Fruit Jam From Several Biological Saati, Elfi Anis; Heidir, Hilwa; Wachid, Moch.; Winarsih, Sri; Haris, Abd.; Nurhakim, Nurhakim
Indonesian Journal of Natural Pigments Vol 1 No 1 (2019): February 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.1.20

Abstract

The study is to investigate the effectiveness of several anthocyanins on quality of papaya jam. It was conducted using a randomized block design, arranged with two factors. The first factor is the anthocyanin with different source with several levels that of control (without pigment), canna flower, grape and skins dragon fruit, factor II is sugar content which is 40%, 50%, and 60%. The results of the study, indicating that there is significant to increase quality products. The best treatment is papaya jam with canna flower anthocyanin pigment and 60% sugar.
Pengaruh Ekstrak Air Daun Kelor Terhadap Kadar Leptin dan Malondialdehyde Lemak Visceral Tikus Wistar yang Dipapar Depo Medroxyprogesterone Acetate Hastuti, Nur Aini Retno; Winarsih, Sri; Dwijayasa, Pande Made
Journal of Issues in Midwifery Vol 2, No 1 (2018)
Publisher : Journal of Issues in Midwifery

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.347 KB) | DOI: 10.21776/ub.JOIM.2018.002.01.4

Abstract

Penggunaan Depo Medroxyprogesterone dikaitkan dengan peningkatan adipogenesis dan oksidatif stress. Daun kelor (Moringa oleifera) adalah antioksidan alami dengan kandungan polifenol. Penelitian ini bertujuan untuk membuktikan ekstrak air daun kelor mempengaruhi kadar leptin dan malondialdehyde (MDA) lemak visceral tikus wistar yang dipapar Depo Medroxyprogesterone Acetate (DMPA). 25 tikus Wistar betina yang dibagi dalam kelompok kontrol negatif (0,2 ml aquades i.m dan 1 cc aquades/oral), kontrol positif (DMPA 2,7mg i.m dan 1 ccaquades/oral), perlakuan DMPA 2,7 mg dengan ekstrak air daun kelor 100 mg/kgBB, 150 mg/kgBB, dan 200 mg/kgBB. Penelitian dilakukan selama empat minggu dan dilakukan pengambilan jaringan lemak visceral. Kadar leptin diukur dengan metode ELISA dan kadar MDA dilakukan dengan metode TBARs menggunakan spektrofotometer. Tikus yang dipapar DMPA (kontrol positif) memiliki rerata kadar leptin dan MDA yang tertinggi dibanding dengan kelompok tikus yang tidak dipapar DMPA (kontrol negatif) ataupun tikus yang mendapat ekstrak air daun kelor (kelompok perlakuan). Uji ANOVA diperoleh p= 0,027  (leptin) dan p=0,001 (MDA) sehingga dapat diintepretasikan terdapat perbedaan yang signifikan pada kadar leptin dan MDA setelah pemberian ekstrak air daun kelor dalam berbagai dosis. Ekstrak air daun kelor menurunkan kadar leptin dan DMPA. Hasil uji Pearson Product Moment p=0,010 (leptin) dan p=0.000 (MDA). Hasil dari penelitian ini menyimpulkan ekstrak air daun kelor mampu menurunkan kadar leptin dan MDA lemak visceral tikus wistar yang dipapar Depo Medroxyprogesterone Acetate
PENGARUH VAKSIN HEAT KILLED Salmonella Typhimurium TERHADAP KADAR TUMOR NECROSIS FACTOR ALPHA (TNF-α) SERUM PADA TIKUS WISTAR JANTAN YANG DIBERI DIET TINGGI LEMAK Winarsih, Sri; Santoso, Putu Adi; Derisna, Anak Agung
Journal of Food and Life Sciences Vol 1, No 2 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfls.2017.001.02.04

Abstract

Salmonella Typhimurium that synthesize phosphorylcholine (PC) was able to induce anti-PC antibody responses that cross-react with the PC on oxidized lipoprotein (Ox-LDL).The purpose of this study was to determine the effect of heat killed bacteria vaccine Salmonella Typhimurium in lowering levels of serum TNF-α in rat fed by high fat diet. This study was an experimental research laboratory. The vaccine was made by heating 108 S. Typhimurium bacteria at temperature of 100°C for 45 minutes on the waterbath. Twenty five male Wistar rats as experimental animals, divided into the negative control group (normal diet) and four treatment groups high fat diet consisting of the positive control group (without vaccination), group A (vaccine 100 μl plus CFA-IFA adjuvant 100 μL), group B (vaccine 100 μL), and group C (CFA-IFA adjuvant 100 μL). The vaccine was injected five times every two weeks, where the vaccine consist of primary injection and four booster injection. The results showed that administration of heat killed S. Typhimurium vaccine plus CFA-IFA can lower the amount of serum levels of TNF-α significantly to the positive control group (p < 0,05). The conclusion is the research proved that heat killed Salmonella Typhimurium vaccine with CFA-IFA adjuvant decreases TNF-α serum which are proinflammantory cytokine. TNF-α is proinflammantory cytokine which has a role in the progression of metabolic syndrome. Further researches about the potential of heat killed S. Typhimurium towards the prevention of the progression of metabolc syndrome are needed to be conducted. Keywords: vaccine, Salmonella Typhimurium, TNF-α
AKTIVITAS ANTIBIOFILM FRAKSI ETIL ASETAT EKSTRAK DAUN PUTRI MALU (Mimosa pudica) PADA BAKTERI METHICILIN-RESISTANT Staphylococcous aureus (MRSA) SECARA IN VITRO Winarsih, Sri; Khasanah, Uswatun; Alfatah, Abu Hasan
Majalah Kesehatan FKUB Vol 6, No 2 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.636 KB) | DOI: 10.21776/ub.majalahkesehatan.006.02.1

Abstract

Methicillin-Resistant Staphylococcus aureus (MRSA) merupakan salah satu bakteri yang dapat membentuk biofilm. Biofilm dapat menghambat kerja antibiotik sehingga dapat menyebabkan resistensi antibiotik. Daun putri malu (Mimosa pudica) memiliki senyawa metabolit sekunder di antaranya yaitu senyawa flavonoid. Senyawa flavonoid yang didapatkan dari fraksi etil asetat daun Mimosa kemungkinan dapat memberikan efek merusak biofilm sekaligus antibakteri bagi bakteri MRSA. Senyawa flavonoid dapat diperoleh melalui proses fraksinasi menggunakan pelarut etil asetat. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian fraksi etil asetat terhadap kerusakan biofilm dan terhadap jumlah koloni bakteri, berdasarkan lama inkubasi. Dosis fraksi etil asetat yang digunakan yaitu 3 mg, 6 mg, 9 mg, 12 mg, 15 mg. Lama inkubasi yang digunakan yaitu satu jam dan dua jam. Jumlah koloni bakteri dihitung menggunakan skor. Skor tertinggi +6 untuk pertumbuhan koloni bakteri yang sangat padat dan skor terendah +1 untuk pertumbuhan koloni bakteri yang renggang. Hasil penelitian menunjukkan bahwa besar kerusakan biofilm seiring dengan dosis fraksi etil asetat (Spearman, r = 0,426, p = 0,01), dan besar kerusakan perlakuan waktu 2 jam lebih tinggi dari pada perlakuan 1 jam (Paired t test, semua dosis menunjukkan p < 0,00). Jumlah koloni menunjukkan penurunan, namun tidak ada perbedaan bermakna antara waktu 1 jam dengan 2 jam (Paired t test, semua dosis p > 0,00). Kesimpulan pada penelitian ini adalah fraksi etil asetat dari ekstrak daun putri malu (Mimosa pudica) dapat merusak biofilm dari bakteri MRSA, dan perlakuan waktu 2 jam menunjukkan kerusakan yang lebih tinggi dari pada 1 jam, namun tidak ada perbedaaan dalam menurunkan jumlah koloni MRSA yang berada dalam lapisan biofilm antara waktu 1 jam dan 2 jam.  
The Effect of Turmeric Decoctum to the Angiogenic Molecules Expression on Chicken Embryo Zahariah, Sultanah; Winarsih, Sri; Baktiyani, Siti Candra Windu; Rahardjo, Bambang; Kalsum, Umi
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.10

Abstract

Turmeric (Curcuma longa) is widely used as herbal medicine, not an exception by pregnant women. Turmeric consumption by expectant mothers requires standard dose, because of its antiangiogenic effect could be harmful on placentation process and embryonic development. This experiment was undertaken to determine the effect of different concentrations of turmeric decoctum to the expression of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) and Angiopoietin 1 (Ang-1) on the 48-hours-old chicken embryo. In this study, turmeric was extracted using decoction method to mimic the common method as adopted by people. The turmeric decoctum were freeze dried into a powder form and was used in preparing the stock solution for 200 ppm (P1), 300 ppm (P2), and 400 ppm (P3) as experimental treatments. The control group (P0) received 2% DMSO without turmeric decoctum. These were administered on the yolk sack of 16 hours incubation of fertile chicken egg by number of 200 µL. After 48 hours incubation, the expression of VEGFR-2 and Ang-1 on the chicken embryo were counted by ImageJ software. The results revealed that there is no significant effect of turmeric decoctum to the expression of VEGFR-2 and Ang-1. This suggested that turmeric decoctum was safe up to 400 ppm on chicken embryo.
Group A β-hemolytic Streptococcus Detection Using Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG) Alluza, Hamid Hunaif Dhofi; Mayasari, Ema Dianita; Prawiro, Sumarno Reto; Winarsih, Sri
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.02

Abstract

Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p < 0.05). The result showed that there was a difference in humoral immune response (IgG) between the groups albeit the difference was not significant (p > 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria. 
p53/Surviving Ratio as a Parameter for Chemotherapy Induction Response in Children with Acute Myeloid Leukemia Lenggana, Rinaldi; Nugroho, Susanto; Winarsih, Sri
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.02

Abstract

Acute myeloid leukemia (AML) is a malignancy that is often found in children. Many studies into the failure of apoptosis function, or programmed cell death, is one of the most important regulatory mechanisms of cellular hemostasis which is closely linked to the development of cancer, are important. Also, regulation of the apoptotic (p53) and anti-apoptotic (surviving) proteins influence treatment outcome. One role of p53 is to monitor cellular stress necessary to induce apoptosis. Surviving (BIRC5) is a group of proteins in the apoptosis inhibitor which works by inhibiting caspase-3. The role of surviving is considered very important in oncogenesis proliferation and cell growth regulation. Chemotherapy in childhood AML can inhibit cell growth and induce slowing as well as stopping the cell cycle. Thus, the aim of this study was to compare p53 and surviving before and after receiving induction chemotherapy in children with AML and also to determine the p53/surviving ratio. Peripheral blood mononuclear cells were collected from AML children before treatment and three months after starting their induction therapy. p53 and surviving were measured by flowcytometry using monoclonal antibodies. Data were analyzed by t-test for comparison between groups and Spearman’s test to find out the correlation between variables with a significant value of p < 0.05. A total of 8 children were evaluated. The intensity of p53 expression was not significantly increased after induction phase chemotherapy (p = 0.224), but surviving expression and the ratio of p53/surviving were significantly increased in the treatment group compared with the levels prior to chemotherapy (p = 0.002, p = 0.034), and there was a strong negative correlation between p53 and surviving after chemotherapy (r = −0.63, p = 0.049).
UJI AKTIVITAS SEDIAAN GEL DAN EKSTRAK LENGKUAS (Alpinia galanga) TERHADAP BAKTERI STAPHYLOCOCCUS EPIDERMIDIS SECARA IN VITRO Prihannensia, Maydia; Winarsih, Sri; Achmad, Anisyah
Pharmaceutical Journal of Indonesia Vol 4, No 1 (2018)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Staphylococccus epidermidis dapat menyebabkan berbagai infeksi kulit pada manusia. Terapi yang sering digunakan adalah antibiotik, salah satunya amoksiklav. Antibiotik terkadang menimbulkan efek samping dan resistensi pada beberapa pasien, sehingga diperlukan terapi alternatif bahan alam yakni rimpang lengkuas (Alpinia galanga) yang mengandung flavonoid dan memiliki aktivitas sebagai antibakteri. Untuk menghantarkan senyawa yang terdapat dalam rimpang lengkuas dan mempermudah penggunaan, maka dibentuk sediaan gel. Metode ekstraksi yang digunakan adalah maserasi menggunakan etanol 70%, dan uji antibakteri dengan metode difusi sumuran. Pada uji Kromatografi Lapis Tipis (KLT), ekstrak lengkuas positif mengandung flavonoid. Gel lengkuas dan ekstrak lengkuas dibuat 3 kelompok konsentrasi yaitu 10%, 15% dan 20%. Parameter yang diamati adalah diameter zona hambat gel dan ekstrak lengkuas setelah diinkubasi selama 18-24 jam pada suhu 37oC. Hasil zona hambat menunjukkan semakin tinggi konsentrasi pada gel dan ekstrak lengkuas, maka semakin besar diameter zona hambat bakterinya. (Korelasi Pearson gel lengkuas R=0,958 dan ekstrak lengkuas R=0,979). Hasil uji t-tidak berpasangan menunjukkan tidak terdapat perbedaan siginifikan antara gel dan esktrak lengkuas (p=0,408). Kesimpulan penelitian adalah terdapat hubungan positif antara peningkatan konsentrasi gel dan ekstrak lengkuas dengan peningkatan diameter zona hambat terhadap bakteri S. epidermidis secara in vitroKata kunci: Lengkuas (Alpinia galanga), Staphylococcus epidermidis, Gel, Ekstrak,  Antibakteri.
Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus Ariel, Dio Giovanni; Winarsih, Sri; Putri, Fitria Febriliani; Erwan, Nabila Erina; Putri, Ajeng Maharani; Cahyono, Alfian Wika; Mardhiyyah, Kana; Fitri, Loeki Enggar; Nugraha, Rivo Yudhinata Brian
Jurnal Kedokteran Brawijaya Vol 31, No 3 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.03.11

Abstract

Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana.  47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.
Efek Antibakteri Ekstrak Daun Katuk (Sauropus androgynus) terhadap Pertumbuhan Salmonella Typhi secara In Vitro Winarsih, Sri; Purwantiningrum, Danik Agustin; Wardhani, Anastasia Shinta
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 15, No 2 (2015)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v15i2.3755

Abstract

Daun Katuk biasa digunakan sebagai obat tradisional terhadap berbagai macam penyakit termasuk mengobati diare. Kandungan daun Katuk yang diperkirakan berperan sebagai antibakteri adalah saponin, flavonoid dan tannin. Penelitian ini bertujuan untuk mengetahui efek antibakteri dari ekstrak daun Katuk secara in vitro. Metode yang digunakan adalah dilusi tabung yang terdiri dari dua tahap yaitu tahap penentuan Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM). Konsentrasi ekstrak daun Katuk yang digunakan adalah 10%, 15%, 20%, 25% dan 30%, sedangkan konsentrasi S. typhi adalah 106 CFU/ml. Hasil menunjukkan bahwa KHM ekstrak daun Katuk terhadap S. typhi sebesar 25% dan KBM nya sebesar 30%. Uji Anova menunjukkan bahwa ekstrak daun Katuk secara signifikan menghambat pertumbuhan S. typhi (p = 0.000). Uji korelasi menggunakan regresi menunjukkan terdapat korelasi negatif  antara konsentrasi ekstrak daun Katuk dengan jumlah koloni S. typhi (p = 0,000; r = - 0,800). Dapat disimpulkan bahwa ekstrak daun Katuk memiliki efek antibakteri terhadap S. Typhi. Semakin tinggi kosentrasi ekstrak daun Katuk, semakin rendah jumlah koloni.Katuk (Sauropus androgynus) leaves is used as a traditional medicine against various diseases include diarrhea. The estimated antibacterial substances of Katuk leaves are saponin, flavonoid and tannin. The aim of this research is to know antibacterial effect of Katuk leaves extract against Salmonella typhi in vitro. Antibacterial effect is determined by tube dilution method, which consists of two steps, namely the determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericide Concentration (MBC). The concentrations of Katuk Leaves extracts are 10%, 15%, 20%, 25%, and 30%. The concentration of S. typhi is 106 CFU/ml. The result shows that MIC and MBC of the Katuk leaves extract are 25% and 30% respectively. Anova test shows that Katuk leaves extract significantly inhibits the growth of S. typhi (p = 0,000). Correlation test by using regression shows that there is a negative correlation between the Katuk leaves extract concentration and the amount of S. typhi colony (p= 0,000; r = - 0,800). The conclusion is that Katuk leaves has antibacterial effect against S. typhi. The higher concentration of Katuk leaf extract, the lower the number of colonies
Co-Authors Abd. Haris Abd. Rasyid Syamsuri Ade Zakiya Tasman Munaf Adisty Dwi Treasa Ahmad Alim Bachri Aisha Rifda Palastri Ajeng Budi Purwati Ajeng Maharani Putri Akbariyanto, Fahrizal Akhid Yulianto Alfatah, Abu Hasan Alfian Wika Cahyono Alluza, Hamid Hunaif Dhofi Almira, Rehan Amelia, Putri Nurhidayati Amir, Nayla Nabilla Tahta Avwina Anastasia Shinta Wardhani Anggawirya, Arin Mantara Anggita Maharani ANISYAH ACHMAD Anisyah Achmad Ardaleni Frista Ariel, Dio Giovanni Arif Widagdo Arsy Arundina Aulanni'am, Aulanni'am Aulia, Aida Salsabila Ayangsari Cahyaningrum Ayuk Lawuningtyas Hariadini Azzahra, Ika Prima Bambang Rahardjo Burhan, M. Cahyaningtyas, Tita Dwi Cahyono, Alfian Wika Caska - Celsy Citra Septiana Daely, Brian Fo’era-era Danik Agustin Purwantiningrum, Danik Agustin Derisna, Anak Agung Dewi Indiastari Dhany Efita Sari Dio Giovanni Ariel Doris Noviani Dwi Yuni Nur Hidayati Elfi Anis Saati Ema Dianita Mayasari Ema P. Yunita Ema Pristi Yunita Ema Pristi Yunita, Ema Pristi Erwan, Nabila Erina Eviana Norahmawati Eviana Norahmawati Febriani, Amelia Dwi Fitria Febriliani Putri Fitria Jannatul Laili Florensy Sauhenda, Angla Frenty Hadiningsih, Eka Hamid Hunaif Dhofi Alluza Hamida, Maretha Hanif Alamudin Manshur Hariyani, Tintin Hastuti, Nur Aini Retno Heidir, Hilwa Huwae, Thomas Erwin C.J I Nengah Kundera Iin Tri Marlinawati Ika Ratnaningrum Insania, Chika Kalsumy, Umi Kana Mardhiyyah Kartika, Bintang Karyono Mintaroem Kesuma, Suryanata Kinasih, Anggraini Lenggana, Rinaldi Lia Sawitri Loeki Enggar Fitri Louise Panggabean, Helena Maemunah Majid, Muhammad Abdul Mardhiyyah, Kana Marni Bawawa Marnina Marnina Maulota, Sally Inggrid Mayasari, Ema Dianita Melati Puspita Sari Muchammad Subali Noto Mukhamad Nooryanto Munayarokh Munayarokh, Munayarokh Murdiono, Jatmiko Murtiningsih Murtiningsih Nabila Erina Erwan Nabilah, Aviana Zuhrotun Narahawarin, Margaretha F Narahawarin, Margaretha F. Nashi Widodo Nikmawati, Nuril Nisa R. Deasury Noorhamdani Novanda, Shabilla Caesar Nugraha, Rivo Yudhinata Brian Nur komalasari Nuraida Fara Rahmani Nurhakim Nurhakim Nurul Istiqomah Oktavia Rahayu Adianingsih Pande Made Dwijayasa Prawiro, Sumarno Reto Prihannensia, Maydia Purwantiningrum, Danik Agustin Putri Ardela, Mayasari Putri, Ajeng Maharani Putri, Fitria Febriliani Rahman, Chientya Annisa Ratnasari, Aprilya Seruni Retty Ratnawati Ribkha Itha Idhayanti Rinaldi Lenggana Riny Natalina Ririn Handayani Rista, Nadia Rivo Yudhinata Brian Nugraha Rosalia Sanarto Santoso Sani, Indra Santoso, Putu Adi Saputra, Hendriek Farhan Saputra, Ridwan Cahya Saputra, Wahyu Joko Sarnita Setyono Yudo Tyasmoro Shafira, Hanna Sijabat, Survey Siti Candra Windu Baktiyani Siti Candra Windu Baktiyani Siti Chunaeni Siti Nurhidayah Sri Andarini Sri Poeranto, Sri Sri Sukasih, Sri Sulistiyani Sulistiyani Sultanah Zahariah Sumarno Reto Prawiro SURYANTI Susanto Nugroho Susanto Nugroho Sutrisno Sutrisno Tamara Gusti Ebtavanny Tatit Nurseta Tatit Nurseta Tatit Nurseta Tatong Harijanto, Tatong Thomas Erwin Christian Junus Huwae Tita Hariyanti Tri Ardyati Tri Yudani Mardining Raras Triana, Salma Ayuning VALENTINA YURINA Wachid, Moch. Wahyuniar Wardhani, Anastasia Shinta Wibowo, Fiska Almayda Widayanti, Isyania Widya, Reta Anggraeni Wiyasa, I Wayan Arsana Yogi Sugito Yohanes Kristantyo Yudhistira Ardana, Yudhistira Yudi Arimba Wani Yulistiani Yulistiani, Yulistiani Zahariah, Sultanah