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All Journal AGRIVITA, Journal of Agricultural Science
Taryono Taryono
Faculty of Agriculture University of Gadjah Mada Jl. Flora Bulaksumur, Yogyakarta, Indonesia, 55281
Agriculture, Biological Sciences & Forestry

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UTILIZATION OF cpDNA SEQUENCES TO IDENTIFY 15 MANGO ACCESSIONS Sukartini Sukartini; Taryono Taryono; Panjisakti Basunanda; Rudi Hari Murti
AGRIVITA, Journal of Agricultural Science Vol 38, No 2 (2016): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i2.767

Abstract

Information about genetic relationship and genetic diversity are an inevitable need in mango breeding program. Base substitution value, genetic distance and grouping of 15 mango accessions based on accessions in chloroplast DNA (cpDNA) among 15 mango accessions were assessed. The samples were originated from Mango Germplasm, Cukur Gondang Research Station of Indonesian Tropical Fruits Research Institute, Pasuruan, East Java. Sequencing of Chloroplast DNA was used to obtain nucleotide sequence data. Paired specific primer rpl20 F - rps12 R and atp F - rbcL R were used for amplification of non-coding area of mango DNA chloroplast and sequencing processes as well. All data were analyzed using Software MEGA 6. The result showed that total numbers and nucleotide base sequences varied among all accessions. All accessions were grouped in five different clusters that might be used as source of parental breeding.
Co-Authors Panjisakti Basunanda Rudi Hari Murti Sukartini Sukartini