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All Journal AGRIVITA, Journal of Agricultural Science Agrotechnology Innovation (Agrinova)
Rudi Hari Murti
Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Veterinary

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Evaluation of Resistant Tea (Camellia sinensis L.) Clones Against Helopeltis bradyi Dimas Tri Asmara; Rudi Hari Murti; Arman Wijonarko; Enik Nurlaili Afifah
AGRIVITA, Journal of Agricultural Science Vol 43, No 3 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i3.2557

Abstract

Helopeltis bradyi is a significant pest that causes yield losses and reduces the quality of tea plantations by piercing and sucking the sap of tea leaves. This pest can be appropriately controlled by using resistant clones. The PGL series (clones) owned by Pagilaran plantation has high yield and quality. However, information their resistance level against H. bradyi is limited. This research was conducted at Pagilaran tea plantation at an altitude of 990 masl. Six PGL clone series (PGL 4, PGL 6, PGL 9, PGL 10, PGL 11, and PGL 15) and control clones (TRI 2025 and Gambung 7) were used as plant materials. Their morphological and biochemical characteristics were determined to evaluate their resistance level against H. bradyi. The results revealed that the score symptoms (under 10%) of PGL 4, PGL 9, PGL 10, PGL 11, and PGL 15 clones were the lowest, so these clones were categorized to be highly resistant. In addition, the total phenol content and trichome density of PGL 4, PGL 10, and PGL 15 clones were the highest. Among them, the PGL 4 clone had the thickest epidermis. These characteristics likely contributed to the morphological and biochemical defence mechanisms of tea plants against H. bradyi.
Comparison of Metabolomics Expression in The Root and Leaf of Resistance and Susceptible Tomato against Root-Knot Nematode Enik Nurlaili Afifah; Rudi Hari Murti; Tri Rini Nuringtyas
AGRIVITA, Journal of Agricultural Science Vol 42, No 3 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i3.2440

Abstract

The resistant tomato against nematode produces various biochemical compounds associated with the defense mechanisms for nematode attack. These compounds allegedly expressed in all cells not only in the infected area. These metabolites are useful for plant development program to point out the candidate traits based on specific metabolites. This study aimed to compare expression of metabolomics as defense mechanism in root and leaf of the plant. Four cultivars consisted of the resistant [GM2 and F1 (a cross GM2 and Hawaii 7996)] and susceptible (Gondol Putih and Gondol Hijau) were used as plant materials. Fifty mg of freeze-dried of root and leaf were taken for assessing 1H-NMR (Nuclear Magnetic Resonance) for metabolomic analysis. Tomato roots resulted in 16 metabolites, while in the leaf detected 15, including amino acid, sugar, and aromatic compounds. Both root and leaf showed the same metabolites that play an important role in nematode-resistant mechanism, these metabolites were α- and β-glucose, and caffeic acid. These compounds had the same concentration within the root and leaf. It is implied that the metabolites associated with defense mechanism of tomato plants against nematode not only expressed locally in the infected area but also produced by not infected tissues throughout the plant.
Effect of Explant Types and Benzyl Amino Purine Concentrations on the In Vitro Regeneration of Several Local Eggplant Cultivars Erniwitama Erniwitama; Taryono Taryono; Rudi Hari Murti
Agrotechnology Innovation (Agrinova) Vol 1, No 1 (2018)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.848 KB) | DOI: 10.22146/agrinova.41750

Abstract

Eggplant as a vegetable is generally fresh consumed. Besides having a high nutritive value, eggplant contains solasonine and solamargine, which act as an antibacterial that can inhibit cancer cells, gastrictic and respiratory disorders. Eggplant also provides a unique system to study in vitro regeneration since it can be induced from different explants by different growth regulators and morphogenetic pathways. This study aims to observe the effect of explant types and BAP concentrations on the in vitro regeneration of three eggplant cultivars, Rimbang,  Tanteloh and Limao. Leaf and hypocotyl explants were cultured on MS semi solid medium supplemented with BAP and IBA in combination. BAP tested at 1, 2 and 3 mgL-1 while IBA tested at 1 mgL-1. Medium containing 1 mgL-1 IBA used for rooting of regenerated shoot. The experiment was arranged in completely randomized design with five replicates. Each replicate consisted of five leaves and hypocotyl explants. Cultures were incubated at 25±10C, 65% humidity, and 16 hours per day photoperiod. Observation was done for 8 weeks of culture. The result indicated that leaf explants showed shoot initiation within 5 – 9 days of cultured, while hypocotyl explants showed the response within 7 – 12 days of cultured. Shoots formation preceded by swollen on the explants surface followed by emerging light green shoot. Leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of shoots regeneration while hypocotyl explants showed less of shoots  in all medium. Similarly, leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of roots. Among the cultivars, Rimbang showed the best response than other cultivars.
Co-Authors Arman Wijonarko Dimas Tri Asmara Enik Nurlaili Afifah Enik Nurlaili Afifah Erniwitama Erniwitama Taryono Taryono Tri Rini Nuringtyas