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All Journal Agrotechnology Innovation (Agrinova)
Taryono Taryono
Center of agrotechnology innovation, Universitas Gadjah Mada, Kalitirto, Berbah, Sleman, Yogyakarta 55573, Indonesia.
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Veterinary

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Effect of Explant Types and Benzyl Amino Purine Concentrations on the In Vitro Regeneration of Several Local Eggplant Cultivars Erniwitama Erniwitama; Taryono Taryono; Rudi Hari Murti
Agrotechnology Innovation (Agrinova) Vol 1, No 1 (2018)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.848 KB) | DOI: 10.22146/agrinova.41750

Abstract

Eggplant as a vegetable is generally fresh consumed. Besides having a high nutritive value, eggplant contains solasonine and solamargine, which act as an antibacterial that can inhibit cancer cells, gastrictic and respiratory disorders. Eggplant also provides a unique system to study in vitro regeneration since it can be induced from different explants by different growth regulators and morphogenetic pathways. This study aims to observe the effect of explant types and BAP concentrations on the in vitro regeneration of three eggplant cultivars, Rimbang,  Tanteloh and Limao. Leaf and hypocotyl explants were cultured on MS semi solid medium supplemented with BAP and IBA in combination. BAP tested at 1, 2 and 3 mgL-1 while IBA tested at 1 mgL-1. Medium containing 1 mgL-1 IBA used for rooting of regenerated shoot. The experiment was arranged in completely randomized design with five replicates. Each replicate consisted of five leaves and hypocotyl explants. Cultures were incubated at 25±10C, 65% humidity, and 16 hours per day photoperiod. Observation was done for 8 weeks of culture. The result indicated that leaf explants showed shoot initiation within 5 – 9 days of cultured, while hypocotyl explants showed the response within 7 – 12 days of cultured. Shoots formation preceded by swollen on the explants surface followed by emerging light green shoot. Leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of shoots regeneration while hypocotyl explants showed less of shoots  in all medium. Similarly, leaf explants cultured on medium supplemented with 3 mgL-1 BAP + 1 mgL-1 IBA showed the maximum number of roots. Among the cultivars, Rimbang showed the best response than other cultivars.
Innovation for Sugarcane Planting Material in Ryland Farming Devita Areifvia Ningsih; Taryono Taryono; Rani Agustina Wulandari
Agrotechnology Innovation (Agrinova) Vol 1, No 1 (2018)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (18734.491 KB) | DOI: 10.22146/agrinova.41772

Abstract

Sugar productivity of sugarcane is dependent on stalk yield and sucrose content which both influenced by planting material. Due to the important of planting material, this study is intended to examine the influence of planting materials i.e. cutting as a control, bud set and bud chip, toward seed quality and the early growth of cane plant in the field. This study was arranged in completly randomized design, with two factors, which are six clones of sugarcane and three types of seedling materials. The results  showed that there was an interactions between both factors on germination rate, vigor index, leaf numbers, internode numbers, tiller numbers, leaf area, root volume, root fresh weight, root dry weight, shoot fresh weight, and shoot dry weight. The early growth of seedling was dependent on the clone. According to shoot fresh weight, cutting was suitable for PS 881 and Bululawang. Bud set for PS 864 clone, whereas bud chip for PSJT 941 and Kidang Kencana. Three seedling materials could be fitted to Bululawang and VMC 86-550, however for dryland farming bud sett and bud chip are preferred because such planting materials can be germinated first at the nursery and then transplantted in the field when there has been enough water content in the soil.
Kemampuan Regenerasi Akar, Kotiledon dan Daun Lima Kultivar Terung Lokal (Solanum melongena L.) Irfan Islami; Taryono Taryono; Rani Agustina Wulandari
Agrotechnology Innovation (Agrinova) Vol 1, No 1 (2018)
Publisher : Agrotechnology Innovation Center, Universitas Gadjah Mada, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.627 KB) | DOI: 10.22146/agrinova.41774

Abstract

Terung merupakan tanaman yang  banyak digunakan sebagai tanaman model dalam perbaikan sifat karena peran dan potensi morfogenik terung yang tinggi. Perannya yang sangat penting dalam menunjang kebutuhan nutrisi dan kesehatan menjadikan banyak penelitian dilakukan untuk mendapatkan jenis terung unggul. Salah satu cara untuk mendapatkan terung jenis unggul adalah budidaya in vitro baik melalui organogenesis langsung maupun tidak langsung dengan induksi keragaman somaklonal. Faktor yang mempengaruhi budidaya in vitro terung yaitu genotipe,  sumber eksplan, dan zat pengatur tumbuh. Namun, dari budidaya in vitro terung yang telah dilakukan tingkat regenerasi yang didapatkan masih rendah. Tujuan dari penelitian ini adalah mengetahui tanggapan regenerasi tiga organ sebagai bahan biakan lima kultivar terung. Perlakuan tersusun oleh 2 faktor yaitu bahan biakan dan kultivar yang ditata dalam Rancangan Acak Lengkap (RAL). Pengamatan kuantitatif dilakukan pada persentase eksplan membentuk tunas, jumlah tunas, tinggi tunas, jumlah daun, panjang daun terbesar, lebar daun terbesar, dan jumlah akar primer. Data kuantitatif hasil penelitian dianalisis menggunakan analisis varians (ANOVA) taraf 5 % dan apabila terdapat sumber ragam yang berbeda nyata, reratanya dibandingkan dengan  Duncan’s Multiple Range Test (DMRT). Pengamatan kualitatif dilakukan pada kalus yang terbentuk, tunas yang terbentuk, tanggapan eksplan akar, tanggapan eksplan daun, tanggapan eksplan kotiledon. Hasil penelitian menunjukkan organ masing-masing kultivar memiliki kenampakan yang beragam. Organ kotiledon menunjukkan regenerasi paling baik berdasarkan pengamatan kualitatif dan kuantitatif khususnya pada  kultivar Lokal Bantul.
Co-Authors Devita Areifvia Ningsih Erniwitama Erniwitama Irfan Islami Rani Agustina Wulandari Rani Agustina Wulandari Rudi Hari Murti