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Pengaruh Faktor Abiotik terhadap Produksi Protease dari Isolat Bakteri M1-23 Mira Rosnawita; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the influence of abiotic factors in producing protease by bacterial isolates M1-23 was conducted from May 2013 to December 2013 in the  Laboratory of Microbiology, Department of  Biology, Andalas University, Padang. This study aimed to find optimal conditions by isolate M1-23 to produce protease. This study consists of two stages. First stage used to produce the best protease and the second used to increase enzim homogenity. The result showed that the highest enzyme activity was  at medium temperature 550 C and pH 7.5. The best homogenity was produced at 125 rpm of agitation process.
Evaluasi Isolat Pseudomonad fluoresens Indigenus dari Rizosfir Berbagai Kultivar Tanaman Pisang Sehat di Lahan Endemik Penyakit Layu Fusarium untuk Pengendalian Penyakit Layu Fusarium: english Sulyanti, Eri; Habazar, Trimurti; Husin, Eti Farda; Nasir, Nasril; Dharma, Abdi
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 2 No. 2 (2018): December 2018
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.2.2.87-96.2018

Abstract

Fusarium wilt diseases caused by Fusarium oxysporum f sp cubense (Foc) is the most important diseases on banana in the world. Once Foc is present in the soil, it cannot be eliminated. The aim of research was to evaluate the potential of Pseudomonad fluorescens indigenous to control Fusarium wilt in planta. This experiment was arranged by completely randomized design with 17 treatments and 10 replications. Sixteen isolates of P. fluorescens indigenous had been successly taken from several different cultivars of banana rhizospheres at endemic area of Fusarium wilt in the centre of banana production in West Sumatra that were introduced to banana seedlings cv Cavendish inoculated with Foc. The variable observed were incubation period, percentage of leaf infection, discoloration of pseudostem and the intensity of damaged corms. The result showed that The PfCvP1 isolate (from rhizosphere of Cavendish at low altitude area) was the most effective to inhibit the development of Fusarium wilt: 97.89 % (prolong incubation period), 67.26 % (reduced infected leaves), 63.63 % (reduced damaged corm), and 72.62 % (reduced disclorotion of pseudostem) and increased plant growth.