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Antagonistic Test of Endophytic Bacteria Against Fusarium oxysporum f.sp. cepae Causes of Moler Disease on Shallots Wihayyu, Afri; Resti, Zurai; Sulyanti, Eri; Darnetty, Darnetty; Khairul, Ujang
CROPSAVER Vol 7, No 1 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i1.54351

Abstract

Fusarium oxysporum f.sp. cepae (FOCe) is a pathogenic fungus causing moler disease in shallots. One method of controlling this disease is by using endophytic bacteria. Endophytic bacteria are bacteria that live in plant tissues without causing disease symptoms. This research aims to obtain the most effective endophytic bacteria in inhibiting the growth of the pathogenic fungus FOCe. The study employs a Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications each. The treatments include Bacillus sp. HI, Bacillus sp. SJI, Bacillus cereus P14, Bacillus cereus Se07, Bacillus subtilis, Serratia marcescens JB1E3, Serratia marcescens ULG1E4, and control. Tests conducted include the antibiosis test of endophytic bacterial cells using the dual culture method and the secondary metabolite test using media poisoning method. The observed parameters include inhibition zone, effectiveness of secondary metabolites, fresh weight, and dry weight of the FOCe fungus. All endophytic bacteria were capable of inhibiting the growth of FOCe. The most effective endophytic bacteria in inhibiting the growth of FOCe were found to be Serratia marcescens ULG1E4 and Serratia marcescens JB1E3. In the antibiosis test of endophytic bacterial cells, Serratia marcescens ULG1E4 exhibited an inhibition zone of 64.44%, while Serratia marcescens JB1E3 showed an inhibition zone of 61.11%. In the secondary metabolite compound test, the effectiveness values for Serratia marcescens ULG1E4 and Serratia marcescens JB1E3 were 95.31% and 95.03%, respectively.
Eksplorasi dan Karakterisasi Rizobakteri untuk Mengendalikan Jamur Fusarium fujikuroi secara In vitro Rahma, Zola Puti; Rahma, Haliatur; Sulyanti, Eri
Jurnal Proteksi Tanaman Vol 8 No 2 (2024): December 2024
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.123-137.2024

Abstract

Rhizobacteria is bacteria that found in the root zone of plants. Rhizobacteria can act as biocontrol agents against Fusarium fujikuroi, the fungus that causes bakanae disease in rice plants. This study aimed to obtain rhizobacteria isolates from the roots of rice plants and to determine their characteristics as biocontrol agents against F. fujikuroi in vitro. This research consisted of two stages: 1) Exploration of rhizobacteria from the roots of rice plants in Padang City, West Sumatera, Indonesia and 2) Antagonistic character of Rhizobacteria against F. fujikuroi in vitro including: antagonistic test, hydrolytic enzyme production test (protease, amylase, and chitinase), and HCN compound production test. Based on the research, 32 rhizobacterial isolates were obtained as candidates for biological agents. All rhizobacterial isolates can suppress the growth of Fusarium fujikuroi in vitro with an inhibition percentage of 19.43-73.53%. The 4 best isolates in suppressing the growth of Fusarium fujikuroi are Kr 2.2 from Kuranji, KG 2.2 from Korong Gadang, PA 2.1 and PA 1.2 from Pasar Ambacang with an inhibition percentage of 60.39-73.53%. The four isolates are also able to produce hydrolysis enzymes (protease, amylase and chitinase) and HCN compounds
Testing Testing the Ability of Epiphytic Yeasts from Rice Leaves to Control Bakanae Disease in Rice Plants: Uji Kemampuan Khamir Epifit Asal Daun Padi untuk Pengendalian Penyakit Bakanae pada Tanaman Padi Hrp, Rosiana Fadilah; Darnetty; Sulyanti, Eri
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 1 (2024): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (1)Desember 2024
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i1.13497

Abstract

Bakanae disease caused by Fusarium fujikuroi causes high yield losses ranging from 3.0-95.4%. The use of epiphytic yeast as a biocontrol agent has the potential to control F. fujikuroi. The aim of this research is to obtain epiphytic yeast species as potential biological control agents to suppress the growth of F. fujikuroi. This research will be carried out in two stages: isolation and in vitro screening. Isolation was carried out using the systematic sampling method and screening using several tests. The parameters observed were morphological characterization of epiphytic yeast and percentage of relative inhibition level against pathogen growth (%). The result showed that shape of the epiphytic yeast cells is round, oval, rod-shaped, and budding. The color of the colonies is pink, milky white, yellow and some form pseudomycelium. The results of observations of the hight percentage of relative inhibition levels against pathogen growth in three consecutive epiphytic yeast isolates were respectively KPSL1.11 (72,44 %), KPSL1.7 (61,37%) dan KPS3 (53,57%).
Biological Control Stem Rot Diseases (Sclerotium Rolfsii) on Peanut (Arachis hypogaea L.) using Arbuscular Mycorrhizal Fungi (AMF) Indigenous Swandi, Fradilla; Sulyanti, Eri; Darnetty, Darnetty
Akta Agrosia Vol 24 No 1 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Arbuscular Mycorrhizal Fungi (AMF) are known to have potential as biological agents controlling plant pathogens. This study aims to obtain indigenous AMF isolates that can suppress the attack of Sclerotium rolfsii which causes stem rot disease in peanut. The method used is an experimental method with a Completely Randomized Design with 5 treatments, namely A : AMF Glomus sp-3 + S. rolfsii; B: AMF Acaulospora sp + S. rolfsii; C: AMF Gigaspora sp + S. rolfsii; D: Combined AMF Glomus sp-3, Acaulospora sp, and Gigaspora sp + S. rolfsii; E: Without AMF + S. rolfsii (Control). Each treatment was repeated 5 times. The data were analyzed using Analysis of Variance (ANOVA) using the Statistix 8 program and the Least Significance Different (LSD) test at a 5% significance level. The results showed that the isolates of Acaulospora sp and Gigaspora sp were able to increase the resistance of peanut plants to stem rot disease (suppressing the incidence and severity of the disease) reaching 100%. Keywords: Arbuscular Mycorrhizal Fungi, indigenous, salicylic acid, Sclerotium rolfsii.
UJI KEMAMPUAN KULTUR FILTRAT PGPB SECARA IN VITRO TERHADAP MORTALITAS DAN PENETASAN TELUR NEMATODA Meloidogyne spp Arifal, Fahkrul; Yanti, Yulmira; Sulyanti, Eri; Harni, Rita
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 9, No 4 (2024): Jurnal Agrohita
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v9i4.19459

Abstract

Nematoda Meloidogyne spp merupakan patogen penyebab penyakit bengkak akar pada tanaman tomat. Alternatif pengendalian penyakit puru akar adalah dengan memanfaatkan kultur filtrat PGPB. Penelitian ini bertujuan untuk mengkaji pengaruh kultur filtra PGPB terhadap mortalitas telur dan penetasan Meloidogyne spp. secara in vitro. Penelitian secara ekperimentas yang terdiri dari 11 perlakuan yaitu Bacillus thuringiensis strain MRSNRZ.3.1, B. subtilis strain MRTDUMBE.3.2.1, B. mycoides strain MRSNUMBE.2.2, B. waihenstephanensis strain RBTLL.3.2, B. cereus strain MRPLUMBE.1.3, Bacillus sp strain MRSPRZ.1.1, Pseudomonas hibiscicola strain MRTLDRZ .2.2, Achromobakter insolitus strain MRBPUMBE.1.3, kontrol air, kontrol NB dan Kontrol Pestisida. Parameter yang diamati adalah mortalitas dan penetasan telur nematoda Meloidogyne spp. Hasil penelitian menunjukkan perlakuan kultur filtrat Bacillus mycoides strain MRSNUMBE.2.2 merupakan perlakuan terbaik dengan tingkat kematian sebesar 98,67% dalam 24 jam dan dengan persentase penetasan telur hanya 10,00%. Kultur filtrat Bacillus subtilis strain MRTDUMBE.3.2.1 juga mencatat hasil yang baik dengan tingkat kematian sebesar 96% dan persentase penetasan telur sebesar 11,33%.
Endophytic Bacteria Consortia as Biocontrol Against Brown Spot Disease (Helminthosporium oryzae) and Growth Promotion in Rice Sulyanti, Eri; Resti, Zurai; Rahma, Haliatur; Darnetty, Darnetty; Khairul, Ujang; Nurhaliza, Nurhaliza; Oktaviani, Silvia
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.1-13.2025

Abstract

The brown spot disease (BSD) caused by Helminthosporium oryzae in rice can lead to yield losses of up to 45 %. Using endophytic bacterial consortia as a biological agent is one of the most potential control methods for resisting the disease. This study aimed to obtain an endophytic bacterial consortium with the best efficacy in controlling BSD and its ability to enhance rice plant growth. Statistical analysis in this study was made using Analysis of Variance (ANOVA) with Duncan’s Multiple Range Test (DMRT) in a randomized experimental design comprising five treatments with five replications. The treatments included: A (Serratia marcescens ULG1E4 + S. marcescens JB1E3), B (S. marcescens ULG1E4 + S. marcescens JB1E3 + Serratia marcescens JB1E2), C (Bacillus sp. SJI + Serratia marcescens ULG1E4), D (positive control), and E (negative control). Testing was conducted in planta, with the introduction of the bacterial consortium (10⁸ cells/mL) via seed and root soaking methods. Subsequently, the leaves of 30-day-old rice plants were inoculated with H. oryzae by spraying a conidial suspension of 10⁶ conidia/ml. Parameters observed included disease progression (incubation period, incidence, and severity), seedling growth metrics (height, leaf number, root length, fresh and dry biomass), and overall plant development (plant height, leaf number, and tiller count). The results demonstrated that the consortium of Serratia marcescens ULG1E4, Serratia marcescens JB1E3, and Serratia marcescens JB1E2 effectively controlled BSD, achieving a 53.69% reduction in disease severity and promoted rice plant growth, evidenced by a 30.63% increase in leaf number