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All Journal Jurnal Kelautan Tropis Journal of Fisheries & Marine
Tony Budi Satriyo
Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology

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Molecular phylogenetic analysis of commercially important Asian monsoon scallop, Amusium pleuronectes (Linnaeus 1758) from Indonesia Eko Hardianto; Tony Budi Satriyo
Jurnal Kelautan Tropis Vol 26, No 3 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i3.18049

Abstract

The Asian monsoon scallop, Amusium pleuronectes, is a key member of shellfish communities that are the most commercially harvested in Asia. Materials were obtained from four sites in Indonesia: Semarang, Bali Island, Maros and Buru Island. Nucleotide sequence analysis was performed on PCR-amplified mitochondrial DNA 16S rRNA. In total, 556-558 base pair nucleotide sequences were obtained from 8 individuals. Molecular analyses revealed that the samples belong to the A. pleuronectes species. Phylogenetic analyses were carried out by comparing the gene sequences of A. pleuronectes haplotypes in Indonesia with gene sequences of the same base pair length and with the of A. japonicum which was used as an outgroup. The phylogenetic trees were consistent and indicated in the two sub-clades. The presence of A. pleuronectes and the phylogenetic status of the A. pleuronectes Indonesian haplotype were reported. Clarifying the phylogenetic status of ecologically important species provides basic information for biosecurity studies for possible future conservation and control programs.
The Effectiveness of Seaweeds as Biofilter for Reducing Wastewater Nutrient and Preventing Water Pollution from Hybrid Grouper Culture Ratih Ida Adharini; Murwantoko Murwantoko; Namastra Probosunu; Riza Yuliratno Setiawan; Tony Budi Satriyo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i2.28105

Abstract

Highlight ResearchWastewater in hybrid grouper culture should be maintain before resirculate and dispose to aquatic environment to avoid eutrophication.Seaweed that is used for biofilter must be observed for its suitability, performance and effectivity.Ulva sp. has the best effectiveness to reduce nutrient in wastewater of hybrid grouper aquaculture by absorbing then stored into thallus.Ulva sp. is proven has the best SGR and adaptability in wastewater of hybrid grouper culture.AbstractWastewater generated from hybrid grouper culture needs to be managed to improve water quality before being recirculated, or discharged in the aquatic environment. Seaweed biofilter has been proposed in wastewater treatment technology for marine fish farming. This study aimed at comparing which of these species i.e. Ulva sp., Sargassum sp., Gelidium sp., and Dictyota sp.  work best to absorb nutrient wastewater hybrid grouper culture. This research utilized these seaweed as treatments and controls with three replications using Completely Randomized Design. A statistical analysis was conducted to investigate the significant differences in the nutrient absorptions among various seaweed species by using ANOVA and least significant difference. Non-parametric tests namely Kruskal-Walis, Mann-Whitney, and t-test were used with confidence interval of 95%. The results revealed that Ulva sp. has the best ability to reduce the Nitrogen 80%, while Dictyota able to reduce  Phosphor 88% by Dictyota sp. generated from wastewater of hybrid grouper culture. While the highest absorbtion of Total Kjeldahl Nitrogen (104%) and phosphate (182%) that stored in thallus were performed by Ulva sp. Ulva sp. had the best performance and highest growth rate (1.9% d-1) as biofilter in hybrid grouper cultivation than other species.
Co-Authors Eko Hardianto Murwantoko . Namastra Probosunu Ratih Ida Adharini Riza Yuliratno Setiawan