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All Journal Jurnal Perikanan dan Kelautan Jurnal Biologi Tropis Leuit, Journal of Local Food Security
Mustahal Mustahal
Universitas Sultan Ageng Tirtayasa
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology

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Determination of Pharmacokinetics and Withdrawal time of Enrofloxacine Antibiotic on Catfish (Clarias gariepinus) Mustahal Mustahal; Dina Solihah; Forcep Rio Indaryanto; Niezha Eka Putri
Jurnal Perikanan dan Kelautan Vol 10, No 1 (2020)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v10i1.8446

Abstract

The purpose of this study was to determine the pharmacokinetics and time of withdrawal of antibiotic enrofloxacin in catfish (Clarias gariepinus) which was attacked by Aeromonas hydrophila. The study was conducted from June to August 2017 at Station for Investigation of Fish Health and Environment, at Pasauran, Serang Regency. The experimental method consisted of two treatments and three replications with each container using 110 fishes. Control treatment was those without antibiotics, while the withdrawal time was those with antibiotics during five days, after five days the withdrawal time container was without antibiotics again. The pharmacokinetics and withdrawal time were determined by the longest time in the organ (ELISA method) and water quality were analyzed descriptively. Data analysis using 95% confidence interval and 50% confidence interval MRL (limited residual maximum). The results showed that the catfish antibiotic was found highest in the renal organ were 360.73 μg / kg before the renal treatment of 10.68 μg / kg and the liver after the highest treatment was 415.75 μg / kg before liver treatment 13.60 μg / kg and increased after treatment. Therefore, the withdrawal time of antibiotic enrofloxacin in catfish was at 16 days.
Apllication of Ovaprim and Oxytocin Hormone for Gonadal Maturation and Embryogenesis of the Pomfret (Colossoma mropomum) Mustahal Mustahal; Mas Bayu Syamsunanrno; Ariq Dwi Wijanarko
Jurnal Perikanan dan Kelautan Vol 10, No 2 (2020)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v10i2.9439

Abstract

The pomfret fish have been widely cultured by fish culturist in Indonesia. However, the gonadal maturation have faced the obstacles with the hormone supplies for the culturists. Therefore, the purpose of this study was to determine the dose of ovaprim and the oxytocin hormone in accelerating gonadal maturation as well the embryogenesis of  the pomfret fish by semi-natural spawning methods. The study was conducted from September to November 2019 at the Laboratory of Fish Seedling Production, at  Baros District, Serang Regency. The number of fish used was 18 fishes (12 males and 6 females), for each spawning treatment have been used 2 males and 1 female. The experiments were using a completely randomized design (CRD) consisting of 2 combinations of treatments and 2 replications. The treatments used were as follows: 0.5 ml / kg ovaprim (K), 0.5 ml / kg ovaprim + 0.25 ml / kg oxytocin (P1), 0.25 ml / kg ovaprim + 0.25 ml / kg oxytocin (P2). The results showed that the use of combination of ovaprim and oxytocin (P1 and P2) was able to stimulate the maturity and spawning of the pomfret fish. The oxytocin injection to induce the spawning have been increased the latency time and increased the fecundity of the pomfret fish. The best hormonal combination treatment in resulting the gonadal maturation and egg hatching was that of  the P2 with the fastest latency time was 614.50 ± 13.44 minutes, the fecundity was 460,134 ± 72,597, fertilization rate  was 80.28 ± 3.11% and hatching rate was 77.08 ± 0.16%.
Evaluation of fermented of Palm Kernel Meal by Saccharomyces cerevisiae in Tilapia Fed Achmad Noerkhaerin Putra; Syamsul Fajri Hidayat; Mas Bayu Syamsunarno; Mustahal Mustahal; Dodi Hermawan; Muh. Herjayanto
Jurnal Perikanan dan Kelautan Vol 10, No 1 (2020)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v10i1.8978

Abstract

Palm kernel meal (PKM) is the potential of raw material for feed fish ingredient because it has good nutritional content and abundant availability in Indonesia. The study was conducted to evaluate of PKM fermented as feedstuffs as well as the effect on feed digestibility of tilapia. This research consisted of 2 trials: the the test of S. cerevisiae fermentation on PKM and digestibility test of PKM as feedstuffs. The first trial used a factorial randomized design consisting of 2 factors: S. cerevisiae dose (0; 0.9; 1.5%) and incubation time (0, 24, 48 hours) with 3 replications. The second trial used a completely randomized design consisting of 3 treatment of test feed, namely A: reference feed, B: reference feed + PKM, C: reference feed + PKM fermented and 4 replications. Fish (initial weight: 5.34 ± 0.14 g) were reared in an aquarium for 40 days with a recirculation system. The results showed that the fermented of 1.5% S. cerevisiae and time incubation for 24 hours recorded the lowest crude fiber content (8.86 ± 0.51%). The highest total digestibility value (P <0.05) was found in treatment A (45.36 ± 2.17%) and C (46.61 ± 2.36%) compared to treatment B (38.46 ± 1, 07%). The highest protein digestibility value significantly (P <0.05) was found in treatments A and C (72.89 ± 4.57%, 74.27±1.26%, respectively) than treatment B (58.70 ± 3.57%). Finally, it can be concluded that the fermentation of PKM by S. cerevisiae can be used as feedstuffs for tilapia feed.
Early Study on Embryogenesis O.woworae at Different Salinities Fani Savitri Agatha; Mustahal Mustahal; Mas Bayu Syamsunarno; Muh. Herjayanto
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2574

Abstract

Oryzias woworae is an endemic fish of Sulawesi. This endemic fish naturally had faced endangered illegal and unlawful capture, therefore it is needed to be protected and conserved by a cultivation system. This research aims to provide basic information related to the cultivation of O. woworae to know the optimum salinity of hatching media and its influence on the hatchability of eggs and the length of time hatching O. woworae. This research was conducted using experimental methods and presented descriptively. The treatment used in this study is 0 ppt, 2-4 pt, 6-8 ppt, 10-12 ppt. The results showed that O. woworae eggs can hatch in salinity 0 ppt until 10-12 ppt and the best salinity for the length of hatching time is treatment of 2-4 ppt which is 7th day 14 hours 20 minutes. Treatment 0 ppt, 2-4 ppt and 6-8 ppt can reach 100%. This can be concluded that the optimum salinity of the hatching rate of Oryzias woworae until 6-8 ppt, but in treatment 10-12 ppt hatchability only gets 80% as some embryos become shrinking. Hatchability per day shows the treatment of 2-4 ppt is the best treatment, on the 7th day as much as 40% and on the 8th day as much as 40%. The optimal salinity for hatching O. woworae eggs is  2-4 ppt treatment, salinity that can expedite the length of hatching time and produce  hatching rate by 100%.  
EFEK PENAMBAHAN PREBIOTIK PADA PAKAN BERBASIS BAHAN BAKU LOKAL UNTUK MENINGKATKAN PERTUMBUHAN DAN KECERNAAN PAKAN IKAN NILA Oreochromis niloticus Achmad Noerkhaerin Putra; Aulia Yuaninda; Syah Banten Anarki; Mas Bayu Syamsunarno; Mustahal Mustahal; Dodi Hermawan; Muhammad Herjayanto
Leuit (Journal of Local Food Security) Vol 1, No 1 (2020)
Publisher : Pusat Unggulan Iptek Ketahanan Pangan Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37818/leuit.v1i1.6899

Abstract

This research was conducted to evaluate the effect of prebiotics addition in feed-based feedstuffs locally on growth and feed digestibility of tilapia. Tilapia (5.19 ± 0.01 g) were reared with a density of 20 fish/container for 45 days. The study consisted of 3 treatments (control, 0.5% prebiotic, 1% prebiotic) with 4 replications.Moringa leaf meal, lamtoro leaf meal and rice bran were used in this study as the local feedstuffs, while the prebiotics used were sweet potato extract. The results showed that the total digestibility and specific growth rate were higher (P<0.05) in prebiotic addition compared to controls. 1% prebiotics treatment showed the best value of protein digestibility (78.24 ± 3.97), specific growth rate (1.57 ± 0.02) and feed efficiency (39.13 ± 0.76) than the other treatments.
Co-Authors Achmad Noerkhaerin Putra Ariq Dwi Wijanarko Aulia Yuaninda Dina Solihah Dodi Hermawan Fani Savitri Agatha Forcep Rio Indaryanto Herjayanto, Muh. Mas Bayu Syamsunanrno Mas Bayu Syamsunarno Niezha Eka Putri Syah Banten Anarki Syamsul Fajri Hidayat